A5073660177- Endometriosis Research and Treatment
- Reproductive System and Pregnancy
- Endometrial and Cervical Cancer Treatments
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Ovarian cancer diagnosis and treatment
- Cancer-related molecular mechanisms research
- Uterine Myomas and Treatments
- Reproductive Biology and Fertility
- Advances in Oncology and Radiotherapy
- Estrogen and related hormone effects
- Chromatin Remodeling and Cancer
- RNA modifications and cancer
- Maternal and fetal healthcare
- Cancer Mechanisms and Therapy
- Biomedical and Engineering Education
- RNA Research and Splicing
- Maternal and Perinatal Health Interventions
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Acute Myeloid Leukemia Research
- Gynecological conditions and treatments
- Sarcoma Diagnosis and Treatment
- Retinoids in leukemia and cellular processes
- Kruppel-like factors research
Indiana University – Purdue University Indianapolis
1999-2023
Indiana University School of Medicine
1999-2023
Indiana University Health
2020-2023
Indiana University Melvin and Bren Simon Comprehensive Cancer Center
2020-2022
Indiana Cancer Consortium
2022
Indiana University Indianapolis
2021
University of Indianapolis
2020-2021
University School
2020
Indiana University
2020
Baylor College of Medicine
2009-2017
Although ovarian cancer is the most lethal gynecologic malignancy in women, little known about how initiates and metastasizes. In last decade, new evidence has challenged dogma that ovary main source of this cancer. The fallopian tube been proposed instead as primary origin high-grade serous cancer, subtype causing 70% deaths. By conditionally deleting Dicer , an essential gene for microRNA synthesis, Pten a key negative regulator PI3K pathway, we show carcinomas arise from mice. these -...
Endometriosis is a common disease seen by gynecologists. Clinical features involve pelvic pain and unexplained infertility. Although endometriosis pathologically characterized endometrial tissue outside the normal uterine location, otherwise not easily explained. Endometriomas, endometriotic cysts of ovary, typically cause distortion anatomy. To begin to understand pathogenesis endometriomas, we describe first transcriptome-microRNAome analysis endometriomas eutopic endometrium using...
MicroRNAs (miRNAs) are small noncoding RNAs that direct gene regulation through translational repression and degradation of complementary mRNA. Although miRNAs have been implicated as oncogenes tumor suppressors in a variety human cancers, functional roles for individual not described clear cell ovarian carcinoma, an aggressive chemoresistant subtype cancer. We performed deep sequencing to comprehensively profile miRNA expression 10 cancer lines compared with normal surface epithelial...
Next-generation sequencing experiments have shown that microRNAs (miRNAs) are expressed in many different isoforms (isomiRs), whose biological relevance is often unclear. We found mature miR-21, the most widely researched miRNA because of its importance human disease, produced two prevalent isomiR forms differ by 1 nt at their 3' end, and moreover end miR-21 posttranscriptionally adenylated noncanonical poly(A) polymerase PAPD5. PAPD5 knockdown caused an increase expression level, suggesting...
Implantation of a blastocyst in the uterus is multistep process tightly controlled by an intricate regulatory network interconnected ovarian, uterine, and embryonic factors. Bone morphogenetic protein (BMP) ligands receptors are expressed pregnant mice, BMP2 has been shown to be key regulator implantation. In this study, we investigated roles BMP type 1 receptor, activin-like kinase 2 (ALK2), during mouse pregnancy producing mice carrying conditional ablation Alk2 (Alk2 cKO mice). absence...
MicroRNAs (miRNAs) are small non-coding RNAs that mediate post-transcriptional gene silencing. Over 700 human miRNAs have currently been identified, many of which mutated or de-regulated in diseases. Here we report the identification novel through deep sequencing RNAome (<30 nt) over 100 tissues cell lines derived from female reproductive organs both normal and disease states. These specimens include ovarian epithelium cancer, endometrium endometriomas, uterine myometrium smooth muscle...
AT-rich interactive domain 1A gene (ARID1A) loss is a frequent event in endometriosis-associated ovarian carcinomas. Endometriosis disease which tissue that normally grows inside the uterus outside uterus, and 50% of women with endometriosis are infertile. ARID1A protein levels were significantly lower eutopic endometrium compared to without endometriosis. However, an understanding physiological effects remains quite poor, function Arid1a female reproductive tract has remained elusive. In...
Significance Why women carrying a pathogenic germline BRCA1 mutation are predisposed to ovarian and breast cancer remains elusive. This study points progesterone as culprit. Generally, -mutation carriers exhibit high yet individually varying levels of during the menstrual cycle. Although not all develop these cancers, them advised undergo prophylactic surgeries at young age (under 40 y 45 y) prevent cancer. Insights from robust in vivo findings this offer novel concept: Targeting signaling...
Significance High-grade serous ovarian cancer (HGSC) is the most common subtype of and typically detected only at advanced stages due to lack effective early screening tools. Fusion genes are among cancer-specific signatures known and, when highly recurrent, they have potential serve as Here we identified BCAM-AKT2 a fusion gene present in 7% HGSC tumors, significant frequency this heterogeneous disease. This results an aberrant kinase whose constant activity contributes formation. Thus,...
Ovarian cancer is the fifth leading cause of death in women. Almost 70% ovarian deaths are due to high-grade serous subtype, which typically detected only after it has metastasized. Characterization further complicated by significant heterogeneity and genome instability displayed this cancer. Other than mutations TP53, common many cancers, highly recurrent recombinant events specific have yet be identified. Using high-throughput transcriptome sequencing seven patient samples combined with...
Recent evidence implicates the orphan nuclear receptor, receptor subfamily 2, group F, member 2 (NR2F2; chicken ovalbumin upstream promoter-transcription factor II) as both a master regulator of angiogenesis and an oncogene in prostate other human cancers.The objective study was to determine whether NR2F2 plays role ovarian cancer dissect its potential mechanisms action.We examined expression healthy ovary cancers using quantitative PCR immunohistochemistry. targeted established cell lines...
Are the transmembrane mucins, MUC1, MUC4 and MUC16, differentially expressed in endometriosis compared with normal endometrium? This study revealed that mucin expression does not vary significantly endometrium during menstrual cycle is altered relative to epithelial marker, cytokeratin-18 (KRT18). Increased serum levels of fragments MUC16 normally dominate apical surface simple epithelia are found several pathological conditions, including endometriosis. Altered gynecologic diseases may...
Our previous whole genome expression analysis of endometriomas suggested dysregulation the ten-eleven translocation genes (TET1, TET2, and TET3), involved in converting 5- methylcytosine to 5-hydroxymethylcytosine (5-hmC). The objective this study was validate TET ectopic eutopic endometrium primary cultures human endometrial stromal fibroblasts (HESF) during vitro decidualization quantify 5-hmC levels patients with endometriosis. Blood, endometrium, endometriotic tissues were collected at...
Epithelial-stromal interactions in the uterus are required for normal uterine functions such as pregnancy, and multiple signaling pathways essential this process. Although Dicer microRNA (miRNA) have been implicated several reproductive processes, specific roles of miRNA development not known. To address regulation key pathways, we generated a conditional knockout postnatal epithelium stroma using progesterone receptor-Cre. These females sterile with small uteri, which demonstrate...
Epigenetic silencing of steroidogenic factor 1 (SF1) is lost in endometriosis, potentially contributing to de novo local steroidogenesis favoring inflammation and growth ectopic endometrial tissue. In this study, we examine the impact SF1 expression eutopic uterus by a novel mouse model that conditionally expresses endometrium. vivo promoted development enlarged glands attenuated estrogen progesterone responsiveness. Endometriosis induction autotransplantation uterine tissue mesenteric...
Endometriosis occurs when endometrial-like tissue grows outside the uterine cavity, leading to pelvic pain, infertility, and increased risk of ovarian cancer. The present study describes optimization characterization cellular spheroids as building blocks for Kenzan scaffold-free method biofabrication proof-of-concept models endometriosis endometriotic microenvironment. spheroid must be a specific diameter (~500 μm), compact, round, smooth withstand biofabrication. Under optimized conditions...
Altered microRNA expression patterns are implicated in the formation of many human diseases, including ovarian cancer. Our laboratory previously created Dicerfl/fl/Ptenfl/fl/Amhr2cre/+ mice, which developed high-grade serous carcinomas originating from mouse fallopian tubes, while neither Dicerfl/fl/Amhr2cre/+ nor Ptenfl/fl/Amhr2cre/+ mice tumors. To explore miRNAs involved tumorigenesis double-knockout (DKO) tumor cell lines were established primary tumors, and most abundant present normal...