A5015365251- Advanced biosensing and bioanalysis techniques
- Analytical chemistry methods development
- Biosensors and Analytical Detection
- Polysaccharides and Plant Cell Walls
- RNA Interference and Gene Delivery
- Clay minerals and soil interactions
- Analytical Chemistry and Chromatography
- Nanoparticle-Based Drug Delivery
- Fungal Biology and Applications
- Nanoplatforms for cancer theranostics
- Pharmacological Effects and Toxicity Studies
- MicroRNA in disease regulation
- Electrochemical sensors and biosensors
- Analytical Chemistry and Sensors
- Selenium in Biological Systems
- Supramolecular Self-Assembly in Materials
- Polysaccharides Composition and Applications
- Iron oxide chemistry and applications
- Phytochemistry and Biological Activities
- CRISPR and Genetic Engineering
- Advanced Nanomaterials in Catalysis
- Electrospun Nanofibers in Biomedical Applications
- biodegradable polymer synthesis and properties
- Carbon and Quantum Dots Applications
- Dendrimers and Hyperbranched Polymers
Changsha Medical University
2023-2025
Central South University
2016-2025
Kunming University of Science and Technology
2024
Changsha University
2015-2016
National University of Singapore
2010
Université de Poitiers
2006
Centre National de la Recherche Scientifique
2006
Stony Brook University
2003
Direct, rapid, sensitive, and selective detection of nucleic acids in complex biological fluids is crucial for medical early diagnosis. We herein combine the trans-cleavage ability clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a with Au-nanobeacon to establish a CRISPR-based biosensor, providing rapid miRNA high speed attomolar sensitivity. In this strategy, we first report that activity CRISPR/cas12a, which was previously reported be triggered only by target ssDNA...
The current state of cancer therapy encourages researchers to develop novel efficient nanocarriers. Halloysite nanotubes (HNTs) are good nanocarrier candidates due their unique nanoscale (40-80 nm in diamter and 200-500 length) hollow lumen, as well biocompatibility low cost. In our study, we prepared a type folate-mediated targeting redox-triggered anticancer drug delivery system, so that Doxorubicin (DOX) can be specifically transported tumor sites the over-expressed folate-receptors on...
The precise detection of extracellular ATP, although a challenging task, is great significance for understanding the related cell processes. Herein, we developed ratiometric DNA nanoswitch by employing tweezer and split aptamer. composed three specially designed ssDNA strands, namely, central strands O1, O2, O3. This can be anchored on membrane cholesterol labeled at 3' end Initially, adopts an open state, separating dual fluorophores giving rise to low FRET (fluorescence resonance energy...
A novel organic–inorganic hybrid nanocomposite was established for the sustained release of an analgesic.
We, herein, indicated for the first time Rnase activities of LbCas12a on linear ssRNA above 11 bases, and hairpin RNA substrates. Meanwhile, bound to ssDNA or exhibited different cleavage efficiencies various substrates, including short ssDNA, DNA, RNA. With DNA as a reporter, we attained detection limit 5 pM 50 targets, respectively. We believe that these findings will pave new avenue expanding reporter toolbox Cas12a-based diagnostics in biosensing biochemistry.
In this work, we present a highly sensitive, specific, and versatile method to quantify miRNA expression by coupling CRISPR-Cas12a with cyclic reverse transcription (CRT), termed as CRISPR-CRT. Each target was first converted amplified into multiple hairpin RT products via CRT. Afterward, the could serve activators initiate collateral cleavage activity of CRISPR-Cas12a. Due above two-stage amplification, assay detect at sub-femtomolar level (LOD, 0.201 fM). Since sequence is double checked:...
A water-compatible molecularly imprinted polymer was prepared by Pickering emulsion polymerization using halloysite nanotubes as stabilized solid particles. During polymerization, we used 4-vinylpyridine monomer, divinylbenzene cross-linking agent, toluene porogen, 2,2-azobisisobutyronitrile initiator, 2,4-dichlorophenoxyacetic acid template to form the oil phase, and Triton X-100 aqueous solution water phase. The characterized Fourier transform infrared spectroscopy scanning electron...