A5082931899- Glaucoma and retinal disorders
- Retinal Development and Disorders
- Ion Transport and Channel Regulation
- Cerebrospinal fluid and hydrocephalus
- Corneal surgery and disorders
- Retinal and Macular Surgery
- Barrier Structure and Function Studies
- Axon Guidance and Neuronal Signaling
- Nerve injury and regeneration
- Retinal Diseases and Treatments
- Intraocular Surgery and Lenses
- Flavonoids in Medical Research
- Protein Kinase Regulation and GTPase Signaling
- Traumatic Brain Injury and Neurovascular Disturbances
- S100 Proteins and Annexins
- Ocular Surface and Contact Lens
- Neurogenesis and neuroplasticity mechanisms
- Cell Adhesion Molecules Research
- Nitric Oxide and Endothelin Effects
- Ophthalmology and Eye Disorders
Johns Hopkins University
2019-2024
Johns Hopkins Medicine
2021-2023
Purpose To delineate responses of optic nerve head astrocytes to sustained intraocular pressure (IOP) elevation in mice. Methods We elevated IOP for 1 day 6 weeks by intracameral microbead injection 4 strains Astrocyte alterations were studied transmission electron microscopy (TEM) including immunogold molecular localization, and laser scanning (LSM) with immunofluorescence integrin β1, α-dystroglycan, glial fibrillary acidic protein (GFAP). proliferation apoptosis quantified Ki67 TUNEL...
Retinal ganglion cell (RGC) replacement holds potential for restoring vision lost to optic neuropathy. Transplanted RGCs must undergo neuroretinal integration receive afferent visual signals processing and efferent transmission. To date, retinal following RGC transplantation has been limited. We sought overcome key barriers transplanted human stem cell-derived integration. Following co-culture ex vivo on organotypic mouse explants, cluster extend bundled neurites that remain superficial the...
A major risk factor for glaucomatous optic neuropathy is the level of intraocular pressure (IOP), which can lead to retinal ganglion cell axon injury and death. The nerve has a rostral unmyelinated portion at head followed by caudal myelinated region. region differentially susceptible IOP-induced damage in rodent models human glaucoma. While several studies have analyzed gene expression changes mouse following injury, few were designed consider regional differences that exist between these...
Purpose: To measure quantitatively changes in lamina cribrosa (LC) cell and connective tissue structure human glaucoma eyes. Methods: We studied 27 19 age-matched non-glaucoma postmortem In 25 eyes, LC cross-sections were examined by confocal multiphoton microscopy to quantify structures identified anti-glial fibrillary acidic protein (GFAP), phalloidin-labeled F-actin, nuclear 4′,6-diamidino-2-phenylindole (DAPI), second harmonic generation imaging of beams. Additional light transmission...
Aquaporin 4 is absent from astrocytes in the rodent optic nerve head, despite high expression retina and myelinated nerve. The purpose of this study was to quantify regional aquaporin channel porcine human mouse (ON). Ocular tissue sections were immunolabeled for aquaporins 1(AQP1), 4(AQP4), 9(AQP9), myelin basic protein (MBP), glial fibrillary acidic (GFAP) alpha-dystroglycan (αDG) their presence retina, lamina, transition zone (MTZ, region just posterior lamina) ON (MON). Semi-...
Purpose To study aquaporin channel expression in astrocytes of the mouse optic nerve (ON) and response to IOP elevation mice lacking 4 (AQP4 null). Methods C57BL/6 (B6) AQP4 null were exposed bead-induced for 3 days (3D-IOP), 1 6 weeks. Mouse ocular tissue sections immunolabeled against aquaporins 1(AQP1), 4(AQP4), 9(AQP9). Ocular was imaged identify normal AQP distribution, ON changes, axon loss after elevation. Ultrastructure examination, cell proliferation, gene expression, transport...
Glaucoma is the leading cause of irreversible blindness worldwide. Elevated intraocular pressure (IOP) one major risk factors for glaucoma onset and progression, available pharmaceutical interventions are exclusively targeted at IOP lowering. However, degeneration retinal ganglion cells (RGCs) may continue to progress despite extensive lowering IOP. A complementary strategy reduction use neuroprotective agents that interrupt process cell death by mechanisms independent Here, we describe an...
Abstract A major risk factor for glaucomatous optic neuropathy is the level of intraocular pressure (IOP), which can lead to retinal ganglion cell axon injury and death. The nerve has a rostral unmyelinated portion at head followed by caudal myelinated region. region differentially susceptible IOP-induced damage in rodent models human glaucoma. While several studies have analyzed gene expression changes mouse following injury, few were designed consider regional differences that exist...
Purpose: The strain response of the mouse astrocytic lamina (AL) to an ex vivo mechanical test was compared between two protocols: eyes that underwent sustained intraocular pressure (IOP) increase and after optic nerve crush. Methods: Chronic IOP elevation induced by microbead injection or crushed in mice with widespread green fluorescence. After 3 days 6 weeks, were inflation tested a published method two-photon fluorescence image AL. Digital volume correlation used calculate strains. Optic...
Optic neuropathies cause irreversible vision loss as retinal ganglion cells (RGCs) die. Transplantation of pluripotent stem cell (PSC)-derived RGCs offers one potential therapeutic avenue to restore in patients suffering from optic neuropathy if the donor neurons survive long-term recipient eye and develop synaptic connections within inner plexiform layer (IPL) subcortical visual centers (1). Thus far, attempts at intravitreal RGC transplantation have been hampered by sequestration on...
Purpose: The purpose of this study was to delineate the neuroprotective mechanisms topical 2% ripasudil (Rip), a Rho kinase (ROCK) inhibitor. Methods: In 340 mice, scheduled Rip or balanced salt solution (BSS) saline drops were intermittently, unilaterally delivered. Intracameral microbead glaucoma (GL) injection increased intraocular pressure (IOP) from 1 day 6 weeks (6W), whereas other mice underwent optic nerve (ON) crush. Retinal ganglion cell (RGC) loss assessed using retinal wholemount...
Abstract Retinal ganglion cell (RGC) replacement and optic nerve regeneration hold potential for restoring vision lost to neuropathy. Following transplantation, RGCs must integrate into the neuroretinal circuitry in order receive afferent visual signals processing transmission central targets. To date, efficiency of RGC retinal integration following transplantation has been limited. We sought characterize spontaneous interactions between transplanted human embryonic stem cell-derived...
Abstract Purpose To study aquaporin channel expression in astrocytes of the mouse optic nerve (ON) and response to IOP elevation mice lacking 4 (AQP4 null). Methods C57BL/6 (B6) AQP4 null were exposed bead-induced for 3 days (3D-IOP), 1 6 weeks. Mouse ocular tissue sections immunolabeled against aquaporins 1(AQP1), 4(AQP4), 9(AQP9). Ocular was imaged identify normal AQP distribution, ON changes, axon loss after elevation. Ultrastructure examination, cell proliferation, gene & transport...