Márcio Roberto Silva

ORCID: 0000-0002-0755-4415
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About
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Research Areas
  • Tuberculosis Research and Epidemiology
  • Mycobacterium research and diagnosis
  • Milk Quality and Mastitis in Dairy Cows
  • Vector-borne infectious diseases
  • Animal Disease Management and Epidemiology
  • Mosquito-borne diseases and control
  • Leprosy Research and Treatment
  • Probiotics and Fermented Foods
  • Dermatologic Treatments and Research
  • Herpesvirus Infections and Treatments
  • Salmonella and Campylobacter epidemiology
  • Health, Nursing, Elderly Care
  • Brucella: diagnosis, epidemiology, treatment
  • Toxoplasma gondii Research Studies
  • Microbial infections and disease research
  • Healthcare Regulation
  • Cytomegalovirus and herpesvirus research
  • Iron Metabolism and Disorders
  • Rural Development and Agriculture
  • Vector-Borne Animal Diseases
  • Food Safety and Hygiene
  • Bacteriophages and microbial interactions
  • Indigenous Health and Education
  • Food Waste Reduction and Sustainability
  • Protein Hydrolysis and Bioactive Peptides

Brazilian Agricultural Research Corporation
2013-2025

Universidade Federal de Juiz de Fora
2013-2024

Universidade Federal da Bahia
2023

Universidade Federal de Minas Gerais
2006-2022

Hospital Universitário - Universidade Federal de Juiz de Fora
2022

Universidade Federal Fluminense
2022

Empresa de Pesquisa Agropecuária de Minas Gerais
2022

Instituto Evandro Chagas
2022

Insper
2022

Universidade Federal de Goiás
2021

ABSTRACT Here, we describe the draft genome sequence of Enterococcus faecium CV167, a strain isolated from raw milk in Brazil that exhibits antimicrobial activity against various pathogens. The has completeness index 99.3%, spans 2,736,418 base pairs, guanine–cytosine content 37.93%, and encodes 2,554 proteins.

10.1128/mra.01036-24 article EN Microbiology Resource Announcements 2025-02-05

BACKGROUND The World Health Organization (WHO) has classified human zoonotic tuberculosis (TB) due to Mycobacterium bovis as a neglected issue in the developing world. In recent cross-sectional study Brazil, three of 189 TB patients presented with coinfection M. and were selected cases for this study. OBJECTIVE aim was evaluate risk factors (RF) an urban area Brazil order guide preventive programmes. METHODS A matched case-control carried out nested within For each cases, 14 age- sex-matched...

10.1590/0074-02760170445 article EN cc-by Memórias do Instituto Oswaldo Cruz 2018-06-11

Global publications on Q fever have increased after the 2007 epidemic in Netherlands. However, epidemiology of fever/coxiellosis Brazil is still poorly understood. Accordingly, there been few studies investigating presence Coxiella burnetii dairy products around world, especially Brazil, where consumption fresh cheese made from raw-milk very high. This study was a random survey to assess prevalence C. by PCR traditional Minas artisanal Serro microregion, which manufactured bovine raw-milk....

10.1016/j.bjid.2020.05.003 article EN cc-by-nc-nd The Brazilian Journal of Infectious Diseases 2020-05-01

Aims: evaluate the medical professionals’ knowledge about Q fever, seeking integrative contributions to public health that may favor formulation of policies and development strategies based on a One Health approach. Methods: this is cross-sectional quantitative study with data collection through structured questionnaire applied face-to-face physicians from various clinical specialties working at three levels care in Brazil. The consisted 25 questions, seven which were specific assessing...

10.15448/1980-6108.2024.1.45474 article EN Scientia Medica 2024-07-23

SUMMARY This study aimed to evaluate the risk factors for tuberculosis (TB) treatment default in a priority city disease control Brazil. A cohort of TB cases diagnosed from 2008 2009 was followed up patients’ entry into three outpatient sites, Juiz de Fora, Minas Gerais (Brazil), until recording outcomes. Drug addiction, alcoholism and site appeared be independently associated with default. Current users crack as hardest drug (odds ratio (OR) 12·25, 95% confidence interval (CI) 3·04–49·26)...

10.1017/s0950268817002631 article EN Epidemiology and Infection 2017-11-27

OBJECTIVE To analyze the cases of tuberculosis and impact direct follow-up on assessment treatment outcomes. METHODS This open prospective cohort study evaluated 504 reported in Sistema de Informação Agravos Notificação (SINAN - Notifiable Diseases Information System) Juiz Fora, MG, Southeastern Brazil, between 2008 2009. The incidence outcomes was compared a group patients diagnosed with directly followed up by monthly consultations during return visits (287) patient for which information...

10.1590/s0034-8910.2015049005304 article EN cc-by Revista de Saúde Pública 2015-01-01

This study examined the feasibility of transcervical embryo recovery after hormonal treatment to induce cervical dilation, following 7-day oestrous synchronization protocol in multiparous Santa Inês ewes. A total 23 cyclic ewes received two doses 37.5 μg d-cloprostenol by latero-vulvar route 7 days apart. After second injection d-cloprostenol, were checked for oestrus (every 12 hr) and then mated fertile rams throughout period. All (latero-vulvar) 1 mg oestradiol benzoate either...

10.1111/rda.13318 article EN Reproduction in Domestic Animals 2018-09-01

In this cross-sectional study, mycobacteria specimens from 189 tuberculosis (TB) patients living in an urban area Brazil were characterised 2008-2010 using phenotypic and molecular speciation methods (pncA gene oxyR pseudogene analysis). Of these samples, 174 isolates simultaneously grew on Löwenstein-Jensen (LJ) Stonebrink (SB)-containing media presented profiles of Mycobacterium tuberculosis, whereas 12 had M. based the DNA analysis formalin-fixed paraffin wax-embedded tissue samples...

10.1590/s0074-02762013000300010 article EN cc-by Memórias do Instituto Oswaldo Cruz 2013-05-01

Mycobacterium tuberculosis is the major cause of in humans. This bacillus gained prominence with occurrence HIV, presenting itself as an important opportunistic infection associated acquired immunodeficiency syndrome (AIDS). The current study aimed to develop a real-time PCR using Eva Green technology for molecular identification M. isolates. primers were designed Rv1510 gene. Ninety nine samples and sixty bovis tested no sample bovine was detected by qPCR. Statistical tests showed...

10.1590/s1517-83822014000400029 article EN Brazilian Journal of Microbiology 2014-12-01

ABSTRACT Objective To compare the performance of Ogawa-Kudoh method with modified Petroff technique in diagnosis pulmonary tuberculosis. Methods A total 205 sputum samples from 166 patients clinical suspicion or under tuberculosis follow-up, seen at a public tertiary care hospital, July 2014 to 2016 were used. All simultaneously processed using and decontamination methods, according recommendations Ministry Health. In statistical analysis, McNemar test Kappa index used, respectively,...

10.1590/s1679-45082018ao4214 article EN cc-by Einstein (São Paulo) 2018-06-11

Abstract Background Q fever is among the top 13 global priority zoonoses, however, it still neglected and under-reported in most of world, including Brazil. Thus, we evaluated seroprevalence risk factors for Coxiella burnetii infections humans from Minas Gerais, a highly urbanised Brazilian state. Methods was searched patient samples (n=437), which were suspected then later confirmed as negative dengue fever, by indirect immunofluorescence technique real-time PCR. Risk spatial clusters both...

10.1093/trstmh/trab113 article EN Transactions of the Royal Society of Tropical Medicine and Hygiene 2021-07-13

Bovine mastitis is an important and costly disease to dairy cattle. Diagnostic methods usually performed in Brazil are somatic cell counts (SCC) milk microbiology. Low bacteria shedding implies no colony growth microbiological tests false negative results. Streptococcus agalactiae Staphylococcus aureus principal pathogens of mixed mastitis. However, S. has a higher bacterial release from the mammary gland than aureus, affecting sensitivity diagnose aureus. This study aimed estimate SCC total...

10.3390/vetsci11060233 article EN cc-by Veterinary Sciences 2024-05-22

Abstract Background Despite high seroprevalence of asymptomatic infection in humans, toxoplasmosis can manifest as a severe systemic disease, occurs the congenital infection. Here we evaluate Toxoplasma among pregnant women highly urbanized area Brazil. Methods A robust seroepidemiological study was conducted using laboratory databases anti-Toxoplasma gondii serological results together with information on age, month/year diagnosis and place residence public health system city Juiz de Fora,...

10.1093/trstmh/traa153 article EN Transactions of the Royal Society of Tropical Medicine and Hygiene 2020-11-19

The article begins by describing a dog with characteristic symptoms of visceral leishmaniasis. A serum sample from this animal was positive indirect immunofluorescence (IIF) performed in anti-Leishmania total IgG 1999. Tissues the same were also polymerase chain reaction (PCR) 2004, identifying Leishmania DNA cerebellum, liver, kidney, and intestine. This is first report autochthonous leishmaniasis county Bom Sucesso, Minas Gerais State, Brazil. finding IIF-positive led to canine...

10.1590/s0102-311x2008000200006 article EN cc-by Cadernos de Saúde Pública 2008-02-01

Bovine tuberculosis (bTB) control programs generally rely on intradermal tuberculin tests for the antemortem diagnosis of Mycobacterium bovis infection in cattle, but these detect only a portion infected animals. The aim present study was to evaluate diagnostic coverage combination bTB techniques known as comparative test (CITT) and an ELISA based recombinant chimera ESAT-6/MPB70/MPB83 antigen cattle. results were compared postmortem findings M. culturing PCR. Paired comparisons all data...

10.1292/jvms.18-0364 article EN Journal of Veterinary Medical Science 2018-10-10

Bactérias patogênicas e resíduos de produtos químicos presentes no ambiente produção podem contaminar o leite causarem problemas tecnológicos à saúde dos consumidores. Esforços para assegurar a qualidade segurança do derivados devem ser iniciados na fazenda isso é necessária adoção boas práticas produção, que são requisitos sistema APPCC (análise perigos pontos críticos controle). O trabalho teve objetivo examinar descrever nível agropecuárias leite. Com ajuda um questionário, entrevistas...

10.22456/1679-9216.16834 article PT cc-by ACTA SCIENTIAE VETERINARIAE 2018-06-27

No Brasil, tem acontecido a dispersão geográfica da Leishmaniose Visceral (LV), conquistando áreas urbanas de muitos municípios, possuindo o cão um papel preponderante nessa urbanização. O artigo descreve inquéritos soroepidemiológicos desencadeados partir identificação do primeiro suspeito e com sorologia positiva para leishmaniose visceral em Bom Sucesso - Minas Gerais, março 1999. Esse município era considerado área indene LV canina. Objetivou-se, então, elucidar situação epidemiológica...

10.22456/1679-9216.14567 article PT cc-by ACTA SCIENTIAE VETERINARIAE 2018-06-27

Neste estudo, foi comparada a contagem de células somáticas (CCS) amostras pareadas leite 86 cabras pelo método eletrônico (Somacount 300) calibrado com padrão vaca, técnica microscópica direta utilizando corante pyronina Y - verde metila. As eram das raças Saanen e Toggenburg um criatório localizado na Zona da Mata Minas Gerais. Em complementação, avaliado o efeito do conservante Bronopol® no sobre CCS. Para as sem conservante, média leituras CCS determinadas microscópio menor diferiu (p...

10.1590/s0103-84782004000500026 article PT cc-by-nc Ciência Rural 2004-10-01
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