A5032161152- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Mycobacterium research and diagnosis
- Herpesvirus Infections and Treatments
- Tuberculosis Research and Epidemiology
- T-cell and Retrovirus Studies
- Brucella: diagnosis, epidemiology, treatment
- Animal Virus Infections Studies
- Microbial infections and disease research
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- Viral Infections and Immunology Research
- Viral Infections and Vectors
- Viral gastroenteritis research and epidemiology
- Plant Virus Research Studies
- Poxvirus research and outbreaks
- Cytomegalovirus and herpesvirus research
- Mosquito-borne diseases and control
- Viral-associated cancers and disorders
- Burkholderia infections and melioidosis
- Plant and Fungal Interactions Research
- Toxoplasma gondii Research Studies
- Salmonella and Campylobacter epidemiology
- Bacillus and Francisella bacterial research
- Infectious Diseases and Mycology
Empresa de Pesquisa Agropecuária de Minas Gerais
2013-2024
Ministry of Agriculture, Livestock, and Food Supply
2014-2024
Centro Universitário de Sete Lagoas
2021-2023
Ministério da Agricultura
2010-2022
Secretaria de Agricultura e Abastecimento
2013
Universidade Federal de Minas Gerais
2012
Companhia Energética de Minas Gerais (Brazil)
2011
A síndrome da apneia-hipopneia obstrutiva do sono (SAHOS) é caracterizada por obstruções repetidas das vias aéreas superiores, resultando em dessaturação oxiemoglobina e microdespertares. Afeta predominantemente homens sua prevalência aumenta com a idade. Entre os fatores de risco estão tabagismo consumo álcool. etiopatogenia envolve redução ativação dos músculos dilatadores faringe durante o sono, favorecendo colapso aéreas. O diagnóstico feito principalmente meio polissonografia, que...
Our study identified strains of the A/H5N1 virus in analyzed samples subsistence poultry, wild birds, and mammals, belonging to clade 2.3.4.4b, genotype B3.2, with very high genetic similarity from Chile, Uruguay, Argentina. This suggests a migratory route for birds across Pacific, explaining phylogenetic relatedness. The Brazilian displayed that had already been previously detected South America. Phylogeographic analysis transmission US viruses Europe Asia, co-circulating other lineages...
In the present study, a nested-PCR system, targeting TbD1 region, involving performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity reactions were assessed with DNA samples extracted from tuberculous non-tuberculous mycobacteria, as well other actinomycetales species directly bovine bubaline terms analytical sensitivity, M. AN5 detected up 1.56 ng 97.6 pg 1.53 reaction...
Post-mortem bacterial culture and specific biochemical tests are currently performed to characterize the etiologic agent of bovine tuberculosis. Cultures take up 90 days develop. A diagnosis by molecular such as PCR can provide fast reliable results while significantly decreasing time confirmation. In present study, a nested-PCR system, targeting rv2807, with conventional followed real-time PCR, was developed detect Mycobacterium tuberculosis complex (MTC) organisms directly from bubaline...
In this cross-sectional study, mycobacteria specimens from 189 tuberculosis (TB) patients living in an urban area Brazil were characterised 2008-2010 using phenotypic and molecular speciation methods (pncA gene oxyR pseudogene analysis). Of these samples, 174 isolates simultaneously grew on Löwenstein-Jensen (LJ) Stonebrink (SB)-containing media presented profiles of Mycobacterium tuberculosis, whereas 12 had M. based the DNA analysis formalin-fixed paraffin wax-embedded tissue samples...
Abstract The prompt and accurate diagnosis of foot-and-mouth disease outbreaks is crucial to curb the swift transmission its causative agent, virus (FMDV), which poses a substantial threat nation’s livestock. This study aimed perform comparative analysis various RT-qPCR methods for detection FMDV. evaluated 3D region demonstrated similar sensitivity specificity. Nevertheless, noticeable distinctions emerged in samples with low RNA concentration. While these can be interchangeably utilized...
Mycobacterium bovis is an important animal pathogen worldwide that parasitizes wild and domesticated vertebrate livestock as well humans. A comparison of the five M. complete genomes from United Kingdom, South Korea, Brazil, States revealed four novel large-scale structural variations at least 2,000 bp. comparative phylogenomic study including 2,483 core genes 38 taxa eight countries showed conflicting phylogenetic signal among sites. By minimizing this effect, we obtained a tree better...
Malaria is an acute febrile disease caused by a protozoan of the genus Plasmodium . Light microscopy (LM) gold standard for diagnosis malaria. Despite this method being rapid and inexpensive, it has low limit detection, which hampers identification parasitemia infections. By using multicopy targets highly sensitive molecular techniques, possible to change scenario. In study, we evaluated performance droplet digital PCR (ddPCR) detect DNA obtained from saliva samples (whole buccal swab) 157...
Leishmania RNA virus 1 (LRV1) is commonly found in South American parasites belonging to the subgenus Viannia, whereas 2 (LRV2) was previously thought be restricted Old-World pathogens of Leishmania.In this study, we investigated presence LRV2 strains (L.) infantum, causative agent visceral leishmaniasis (VL), originating from different hosts, clinical forms, and geographical regions.A total seventy-one isolates were screened for using semi-nested reverse transcription-polymerase chain...
This study aimed to develop and validate real-time PCR for the diagnosis of Mycobacterium bovis isolates.Two hundred seventy-four M. isolates 156 tuberculosis were tested.Both qPCRs amplified all 274 samples, but none samples.The qPCR PE-PGRS 20 had 91% efficiency a detection limit 0.32 ng (sensitivity specificity "Mbovis.100"were 99.64 100%, respectively).The RD4 100% efficiency, 4 pg (diagnostic sensitivity 100 100%.The tests performed using extraction sets, 3 kits, with range equipment;...
Vesicular diseases are of high importance for livestock, primarily because foot-and-mouth disease (FMD), which is a high-morbidity that generates direct losses caused by low milk production, weight loss, and indirect the need sanitary barriers. Other vesicular also livestock impacts or their clinical signs may be confused with those FMD. We report herein detection multiple infections in cattle suspected Brazilian states Amazonas (AM), Mato Grosso (MT), Roraima. Thirty-seven epithelial...
Suid herpesvirus 1 (SuHV-1) is the causative agent of pseudorabies (PR), a disease great importance due to huge losses it causes in swine industry. The aim this study was determine method for genotyping SuHV-1 based on partial sequences gene coding glycoprotein C (gC) and elucidate possible reasons variability region. A total 109 gCsequences collected from GenBank were divided into five major groups after reconstruction phylogenetic tree by Bayesian inference. analysis showed that portion gC...
For the definitive diagnosis of bovine tuberculosis, isolation etiologic agent is required. However, there no consensus on best methodology for Mycobacterium bovis in Brazil. This study evaluated most used decontaminants and culture media country, order to identify combination Brazilian samples. Three - 2% sodium hydroxide (w/v), 0.75% hexadecylpiridinium chloride (w/v) 5% sulphuric acid (v/v) four 7H11 Middlebrook with additives OADC supplement "A" (7H11 A), same another trademark B),...
In the last decade there was a significant increase dairy goat participation in world and Brazilian agricultural scenario. Minas Gerais state is third largest producer of milk Brazil. Paratuberculosis chronic intestinal disease that mostly affects ruminants can become public health problem due to possible association with Crohn's disease. It transmitted by ingesting Mycobacterium avium subsp. paratuberculosis (MAP) contaminated food or water. recent years, development molecular techniques,...
Abstract Background The allergic test of mallein is one the most frequently used tests, together with Complement Fixation Test (CFT), for diagnosis glanders in endemic areas. Mallein, a purified protein derivative (PPD), produced similarly to PPD tuberculin and end product primarily proteic antigen, which only poorly purified. immuno-allergic activity believed be due high molecular weight group proteins present antigen. To improve quality terms sensitivity specificity, new method production...