A5007883658- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Viral Infections and Vectors
- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Vector-Borne Animal Diseases
- Insect-Plant Interactions and Control
- Insect symbiosis and bacterial influences
- Bacteriophages and microbial interactions
- Plant Disease Resistance and Genetics
- Insect and Pesticide Research
- Antimicrobial Resistance in Staphylococcus
- Toxin Mechanisms and Immunotoxins
- Antibiotic Resistance in Bacteria
- Microbial Community Ecology and Physiology
- Parasites and Host Interactions
- Bacterial Genetics and Biotechnology
- Evolution and Genetic Dynamics
- Nematode management and characterization studies
University of Ostrava
2014-2025
Central European Institute of Technology – Masaryk University
2019-2025
Central European Institute of Technology
2019-2024
Washington University in St. Louis
2017
Czech Academy of Sciences, Biology Centre
2017
Institute of Parasitology
2017
The co-infection cases involving dixenous Leishmania spp. (mostly of the L. donovani complex) and presumably monoxenous trypanosomatids in immunocompromised mammalian hosts including humans are well documented. main opportunistic parasite has been identified as Leptomonas seymouri sub-family Leishmaniinae. molecular mechanisms allowing a insects to withstand elevated temperature substantially different conditions vertebrate tissues not understood. Here we demonstrate that is adapted for...
Significance Largely overlooked, the viruses of protists have started to attract more attention. Several family Totiviridae are currently implicated in increased pathogenicity parasitic protozoa such as Leishmania vertebrate hosts. We conducted a broad survey RNA within trypanosomatids, one iconic groups protists. These revealed several previously unidentified viral taxa including designated “ Leishbunyaviridae ” and highly divergent virus termed “Leptomonas pyrrhocoris ostravirus 1.” Our...
Significance The bacterial cell wall peptidoglycan is essential for maintenance of viability and yet dynamic, permitting growth division. Peptidoglycan synthesis inhibited by important antibiotics, including β-lactams vancomycin. Using the human pathogen Staphylococcus aureus , we have examined homeostatic mechanisms how their interruption leads to death. This has revealed two antibiotic-induced killing mediated specific hydrolases, both involving appearance holes that span entire thickness...
RNA viruses play an important role in Leishmania biology and virulence. Their presence was documented three (out of four) subgenera. Sauroleishmania reptiles remained the only underinvestigated group. In this work, we analyzed viral occurrence spp. detected out seven isolates under study. These were two families-Narnaviridae Totiviridae. Phylogenetic inferences demonstrated that totiviruses from L. adleri tarentolae group together within a larger cluster LRV2s, while narnavirus gymnodactyli...
In the present work, we investigated molecular mechanisms governing thermal resistance of a monoxenous trypanosomatid Crithidia luciliae thermophila, which reclassified as separate species C. thermophila. We analyzed morphology, growth kinetics, and transcriptomic profiles flagellates cultivated at low (23°C) elevated (34°C) temperature. When maintained high temperature, they grew significantly faster, became shorter, with genes involved in sugar metabolism mitochondrial stress protection...
In this work, we analyzed viral prevalence in trypanosomatid parasites (
Here we report sequence and phylogenetic analysis of two new isolates Leishmania RNA virus 2 (LRV2) found in major isolated from human patients with cutaneous leishmaniasis south Uzbekistan. These virus-infected flagellates were the same region Uzbekistan viral sequences differed by only nineteen SNPs, all except one being silent mutations. Therefore, concluded that they belong to a single LRV2 species. New viruses are closely related LRV2-Lmj-ASKH documented Turkmenistan 1995, which is...
The globally distributed marine alga
In this work, we describe the first Leishmania-infecting leishbunyavirus—the virus other than Leishmania RNA (LRV) found in trypanosomatid parasites. Its host is martiniquensis, a human pathogen causing infections with wide range of manifestations from asymptomatic to severe visceral disease. This (LmarLBV1) possesses many characteristic features leishbunyaviruses, such as tripartite organization its genome, ORFs encoding RNA-dependent polymerase, surface glycoprotein, and nucleoprotein on...
Leishmania spp. are important pathogens causing a vector-borne disease with broad range of clinical manifestations from self-healing ulcers to the life-threatening visceral forms. Presence RNA virus (LRV) confers survival advantage these parasites by suppressing anti-leishmanial immunity in vertebrate host. The two viral species, LRV1 and LRV2 infect species subgenera Viannia Leishmania, respectively. In this work we investigated co-phylogenetic patterns leishmaniae their viruses on small...
Abstract Background Trypanosomatids are parasitic flagellates well known because of some representatives infecting humans, domestic animals, and cultural plants. Many trypanosomatid species bear RNA viruses, which, in the case human pathogens Leishmania spp., influence course disease. One close relatives leishmaniae, Leptomonas pyrrhocoris , has been previously shown to harbor viruses groups not documented other trypanosomatids. At same time, this a worldwide distribution high prevalence...
Abstract Trypanosomatids (Euglenozoa) are a diverse group of unicellular flagellates predominately infecting insects (monoxenous species) or circulating between and vertebrates plants (dixenous species). Monoxenous trypanosomatids harbor wide range RNA viruses belonging to the families Narnaviridae, Totiviridae, Qinviridae, Leishbuviridae, putative tombus-like viruses. Here, we focus on subfamily Blastocrithidiinae, previously unexplored divergent monoxenous comprising two related genera:...
Leishmania RNA virus 1 (LRV1) is commonly found in South American parasites belonging to the subgenus Viannia, whereas 2 (LRV2) was previously thought be restricted Old-World pathogens of Leishmania.In this study, we investigated presence LRV2 strains (L.) infantum, causative agent visceral leishmaniasis (VL), originating from different hosts, clinical forms, and geographical regions.A total seventy-one isolates were screened for using semi-nested reverse transcription-polymerase chain...
Abstract L-BC virus persists in the budding yeast Saccharomyces cerevisiae , whereas other viruses from family Totiviridae infect a diverse group of organisms including protists, fungi, arthropods, and vertebrates. The presence totiviruses alters fitness host organisms, for example, by maintaining killer system or increasing virulence Leishmania guyanensis . Despite importance their survival, there is limited information about Totivirus structure assembly. Here we used cryo-electron...
Leishmania parasites cause a variety of symptoms, including mucocutaneous leishmaniasis, which results in the destruction mucous membranes nose, mouth, and throat. The species carrying RNA virus 1 (LRV1), from family Totiviridae, are more likely to severe disease less sensitive treatment than those that do not contain virus. Although importance LRV1 for severity leishmaniasis was discovered long time ago, structure remained unknown. Here, we present cryo-electron microscopy reconstruction...
Leishmania is a genus of the family Trypanosomatidae that unites obligatory parasitic flagellates causing variety vector-borne diseases collectively called leishmaniasis. The symptoms range from relatively innocuous skin lesions to complete failures visceral organs. disease exacerbated if parasite harbors RNA viruses (LRVs) Pseudototiviridae . Screening novel isolate L braziliensis , we revealed it possesses not toti-, but bunyavirus Leishbuviridae To best our knowledge, this very first...
The presence of Leishmania RNA virus 1 (LRV1) enables protozoan parasites to cause more severe disease than the virus-free strains. structure LRV1 virus-like particles has been determined previously, however, virion not characterized. Here we used cryo-electron microscopy and single-particle reconstruction determine structures empty particle isolated from guyanensis resolutions 4.0 Å 3.6 Å, respectively. capsid is built sixty dimers proteins organized with icosahedral symmetry. genomes...