A5037796346- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Acute Myeloid Leukemia Research
- Urticaria and Related Conditions
- Insect Pest Control Strategies
- Blood disorders and treatments
- Toxin Mechanisms and Immunotoxins
- Chronic Lymphocytic Leukemia Research
- Hematological disorders and diagnostics
- Suicide and Self-Harm Studies
- Vector-borne infectious diseases
- Autoimmune and Inflammatory Disorders Research
- Lymphoma Diagnosis and Treatment
- Immunodeficiency and Autoimmune Disorders
- Eosinophilic Disorders and Syndromes
- Reconstructive Surgery and Microvascular Techniques
- Insect and Pesticide Research
- Parkinson's Disease and Spinal Disorders
- Dermatological diseases and infestations
- Child and Adolescent Psychosocial and Emotional Development
- Obesity, Physical Activity, Diet
- Advanced Biosensing Techniques and Applications
- Glycosylation and Glycoproteins Research
- Retinoids in leukemia and cellular processes
- Vascular Malformations and Hemangiomas
Universidade de São Paulo
2018-2025
Universidade Estadual Paulista (Unesp)
2025
University of Buenos Aires
2024
New York University
2023-2024
Temple University
2024
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2020-2023
University of Toronto
2021
Mercy Hospital
2020
Secretaria da Educação do Estado da Bahia
2020
San Antonio College
2020
Leishmaniasis, a complex disease caused by protozoal parasites of the genus Leishmania, presents various clinical forms, particularly cutaneous form. Treatment is typically performed with pentavalent antimonial and amphotericin B, both which have severe side effects that hinder patient compliance. This emphasizes need for development new, effective, safe treatments. In this study, leishmanicidal activity methanolic extract, an alkaloid-enriched fraction dicentrine, main alkaloid leaves...
Leishmania (Leishmania) infantum chagasi infections range from asymptomatic (AS) to severe visceral leishmaniasis (VL). One of the manifestations is an atypical non-ulcerated cutaneous (NUCL), which occurs in some locations Central America with few cases VL. We conducted a transcriptomic analysis cell-mediated immunity (CMI) on blood samples NUCL, AS, VL patients Amapala, Honduras, and healthy controls. RNA-seq revealed similar perturbation gene expression NUCL AS. Eight signatures CMI were...
ABSTRACT In Honduras, Leishmania (Leishmania) infantum chagasi, the etiological agent of visceral leishmaniasis (VL), is responsible for non-ulcerated cutaneous (NUCL). We characterized NUCL and VL Honduran strains to understand intraspecies infectivity. Based on in-vitro assays, we aimed elucidate certain host-parasite interactions in isolates through a hamster model. To assess capacity these infect peritoneal macrophages, exposed them promastigotes from patients at varying temperatures...
Macrophages play important roles in the innate and acquired immune responses against Leishmania parasites. Depending on subset activation status, macrophages may eliminate intracellular parasites; however, these host cells also can offer a safe environment for replication. In this sense, fate of parasite be influenced by phenotype infected macrophage, linked to subtype classically activated (M1) or alternatively (M2) macrophages. present study, M1 M2 macrophage subsets were analyzed...
Abstract Visceral leishmaniasis is spreading in Brazil where the main vector of its agent, Leishmania infantum Nicolle, 1908, Lutzomyia longipalpis (Lutz & Neiva, 1912) species complex (Diptera: Psychodidae: Phlebotominae), on which many activities visceral surveillance program are based. However, there areas canine, and/or human cases have been occurring without presence this as western part Greater São Paulo Metropolitan region, Embu das Artes municipality situated. In area, Pintomyia...
In Honduras visceral leishmaniasis and non-ulcerated or atypical cutaneous (NUCL) are caused by the species Leishmania (L.) infantum chagasi. NUCL is most common clinical form in southern regions of country, mainly affecting young. view lack knowledge about pathogenesis disease pattern L. (L) chagasi individuals affected NUCL, aim present study was to describe detail histopathological features skin lesion parasite. Biopsies from human lesions with a positive parasitological diagnosis were...
In Honduras, Leishmania (L.) infantum chagasi causes both visceral leishmaniasis (LV) and nonulcerated or atypical cutaneous (NUCL). NUCL is characterized by mononuclear inflammatory infiltration of the dermis, composed mainly lymphocytes followed macrophages with discrete parasitism. Considering that little known about pathogenesis NUCL, aim this study was to evaluate regulatory response in situ skin lesions patients affected NUCL. Biopsies (n = 20) from human nonulcerative were collected...
Leishmania RNA virus 1 (LRV1) is commonly found in South American parasites belonging to the subgenus Viannia, whereas 2 (LRV2) was previously thought be restricted Old-World pathogens of Leishmania.In this study, we investigated presence LRV2 strains (L.) infantum, causative agent visceral leishmaniasis (VL), originating from different hosts, clinical forms, and geographical regions.A total seventy-one isolates were screened for using semi-nested reverse transcription-polymerase chain...
Although Leishmania infantum is well-known as the aethiological agent of visceral leishmaniasis (VL), in some Central American countries it may cause atypical non-ulcerated cutaneous (NUCL). However, mechanisms favoring its establishment skin are still unknown. Lipophosphoglycan (LPG) major multivirulence factor involved parasite-host interaction. In case viscerotropic L. infantum, causes an immunosuppression during interaction with macrophages. Here, we investigated biochemical and...
Leishmania (Leishmania) infantum is the etiological agent of both American visceral leishmaniasis (AVL) and non-ulcerated cutaneous (NUCL) in Honduras. Although AVL most severe clinical form infection, recent studies have shown that human immune response to parasite infection can result a clinical-immunological spectrum. The overall prevalence rate profiles L. (L.) Amapala municipality, South Honduras was determined. We examined 576 individuals with diagnosis based on combined ELISA...
Abstract Skin lesions in nonulcerated cutaneous leishmaniasis (NUCL) caused by Leishmania (L.) infantum chagasi are characterized a mononuclear inflammatory infiltrate the dermis, which is composed mainly of lymphocytes, followed macrophages, few plasma cells and epithelioid granulomas with mild tissue parasitism. Previous studies have shown that main population lymphocytes present dermal CD8 + T cells, CD4 correlated IFN‐γ cells. To improve knowledge cellular immune responses NUCL, skin...
American tegumentary leishmaniasis (ATL) is highly endemic in the Amazon basin and occurs all South countries, except Chile Uruguay. Most Brazilian ATL cases are due to Leishmania (Viannia) braziliensis , however other neglected Amazonian species being increasingly reported. They belong subgenus L . information on suitable models understand immunopathology scarce. Here, we explored use of golden hamster Mesocricetus auratus its macrophages as a model for species. We also studied interaction...
This work reports on the whole-genome sequencing of Leishmania infantum chagasi from Honduras (Central America) and Brazil (South America).
Visceral leishmaniasis (VL), also known as kala-azar, is an anthropozoonotic disease affecting human populations on five continents. Aetiologic agents belong to the Leishmania (L.) donovani complex. Until 1990s, three leishmanine parasites comprised this complex: L. Laveran & Mesnil 1903, infantum Nicolle 1908, and chagasi Lainson Shaw 1987 (=L. Cunha Chagas 1937). The VL causal agent in New World (NW) was previously identified chagasi. After development of molecular characterization,...
In Central America, infection by Leishmania (Leishmania) infantum chagasi causes visceral leishmaniasis and non-ulcerated cutaneous (NUCL). This work aimed to evaluate the participation of subpopulations antigen-presenting cells in skin lesions patients affected NUCL through double-staining immunohistochemistry using cellular intracellular markers. Twenty-three biopsies from were used. Histological sections stained HE used for histopathological study. Immunohistochemical studies performed...
Dogs are considered to be the main domestic reservoir associated with transmission of Leishmania (L.) infantum chagasi humans in endemic areas visceral leishmaniasis America. However, little is known about role canines as a source infection nonulcerated cutaneous (NUCL). Therefore, objective present study was investigate dogs possible parasite Southern Honduras. (n = 107) living individuals affected by NUCL were clinically examined and biological material collected for parasitological...
Abstract Background The two most abundant sand fly species on the Honduran Pacific coast are Lutzomyia ( ) longipalpis and Pintomyia Pifanomyia evansi . Both known vectors of Leishmania infantum , etiological agent visceral leishmaniasis (VL) in Americas. Although VL non-ulcerative cutaneous (NUCL) endemic versant Central American Pacific, latter is frequent manifestation there. We evaluated circulation spp. El Tigre Island, an area NUCL. Results collected 222 specimens six species. Lu. (180...
La leishmaniasis es una enfermedad infecciosa causada por un protozoo del género Leishmania. En Honduras importante causa de morbilidad. El propósito estudio fue caracterizar la eco epidemiología cutánea no ulcerada (LCNU) en Honduras. Se seleccionaron 4 municipios endémicos a LCNU (Amapala, San Francisco Coray, Reitoca y Alubarén). Para realizar captura Lutzomyia se usaron trampas miniatura tipo CDC, los individuos capturados fueron identificados según criterios Young & Duncan....
In some central-American countries, Leishmania (L.) infantum chagasi infection can cause non-ulcerated or atypical cutaneous leishmaniasis (NUCL) in addition to the classic clinical form, visceral (VL). Little is known about host-parasite relationship that contribute determination of one another form. The present study had objective evaluate humoral and cellular immunity sera individuals affected by NUCL improve comprehension this interaction. Based on laboratory diagnosis, serum 80 was...