Can we reconstitute physiologically relevant 3-dimensional (3D) microengineered endometrium in-vitro model?Our representative vascularised on-a-chip closely recapitulates the endometrial microenvironment that consists of three distinct layers including epithelial cells, stromal fibroblasts and endothelial cells in a 3D extracellular matrix spatiotemporal manner.Organ-on-a-chip, multi-channel microfluidic cell culture system, is widely used to investigate responses organ systems.The device...

Successful implantation requires the synchronization of viable embryonic development with endometrial receptivity. The mechanisms allowing for initiation crosstalk between embryo and endometrium remain elusive, however recent studies have revealed alterations in microRNAs (miRs) women suffering repeated failure; one altered miRs is miR-145. We assessed role miR-145 its target, IGF1R, early implantation. overexpression IGF1R knockdown were achieved Ishikawa cells. QPCR, western blotting 3′UTR...

Abstract Tissue‐specific decellularized extracellular matrix recapitulates the complexity of natural ECMs, creating an organ‐specific microenvironment based on its intrinsic characteristics. Here, hydrogels containing uterus‐derived (UdECMs) from endometrium‐specific layer or entire uterus are developed. UdECMs serve as effective biomaterials, displaying that intrauterine UdECM administration induces endometrial regeneration and fertility enhancement. Moreover, alters profile killer cell...

Background: Asherman's syndrome (AS) is a dreadful gynecological disorder of the uterus characterized by intrauterine adhesion with severe fibrotic lesions, resulting in damaged basalis layer infertility.Despite extensive research on overcoming AS, evidence-based effective and reproducible treatments to improve structural functional morphology AS endometrium have not been established.Methods: Endometrial organoids generated from human or mouse endometrial tissues were transplanted into...

Abstract DNA repair gene defects are found in virtually all human glioblastomas, but the genetic evidence for a direct role remains lacking. Here we demonstrate that combined inactivation of XRCC4 non-homologous end-joining (NHEJ) and p53 efficiently induces brain tumours with hallmark characteristics proneural/classical glioblastoma. The murine exhibit PTEN loss function instigated by reduced mRNA, increased phosphorylated stability as consequence aberrantly elevated CK2 provoked ablation...

Various adjuvants have been tested clinically for patients with problems embryo implantation during in vitro fertilization (IVF)-embryo transfer (ET). Vitamin D3, an essential modulator of various physiological processes, has received attention as important adjuvant successful pregnancy, many studies shown a strong association between vitamin D deficiency and failure fetal growth restriction. However, widely utilized different protocols, resulting non-reproducible debatable outcomes. In the...

Abstract Successful pregnancy inevitably depends on the implantation of a competent embryo into receptive endometrium. Although many substances have been suggested to improve rate targeting enhancement endometrial receptivity, currently there rarely are effective evidence-based treatments prevent or cure this condition. Here we strongly suggest minimally-invasive intra-uterine administration embryo-secreted chemokine CXCL12 as an therapeutic intervention. Chemokine derived from pre- and...

Adequate endometrial growth is a critical factor for successful embryo implantation and pregnancy maintenance. We previously reported the efficacy of intrauterine administration botulinum toxin A (BoTA) in improving angiogenesis rates implantation. Here, we further evaluated its potent therapeutic effects on uterine structural functional repair elucidated underlying molecular regulatory mechanisms. This study demonstrated that murine model thin endometrium was successfully established by...

BACKGROUND Activation of angiotensin (ANG) II type 1 receptors (AT1R) promotes vasoconstriction, inflammation, and renal dysfunction. In this study, we addressed the ability azilsartan (AZL), a new AT1R antagonist, to modulate levels plasma ANG-(1–7) epoxyeicosatrienoic acids (EETs) 20-hydroxyeicosatetraenoic acid (20-HETE). METHODS Sprague-Dawley rats were infused with ANG (125ng/min) or vehicle (VEH). AZL (3mg/kg/day) VEH was administered starting day prior infusion. On 10, obtained for...

The aim of this study was to investigate microRNAs (miRNAs) related follicle-stimulating hormone (FSH) responsiveness using miRNA microarrays and identify their target genes determine the molecular regulatory pathways involved in FSH signaling KGN cells.To change cellular FSH, cells were treated with receptor (FSHR)-specific small interfering RNA (siRNA) followed by FSH. expression profiles determined through microarray analysis. Potential selected miRNAs predicted bioinformatics tools,...

This study is designed to investigate the effects of increased progesterone (P4) levels on quality retrieved oocytes and embryos during IVF. retrospective analysis included 982 all-freezing in vitro fertilization (IVF) cycles (conducted between November 2019 June 2020 at CHA Fertility Center Bundang, South Korea) which serum P4 were measured day human chorionic gonadotropin (hCG) administration. Our revealed that hCG administration are strongly associated with rates oocyte maturation,...

Endometrial fibrosis, the primary pathological feature of intrauterine adhesion, may lead to disruption endometrial tissue structure, menstrual abnormalities, infertility, and recurrent pregnancy loss. At present, no ideal therapeutic strategy exists for this fibrotic disease. Eupatilin, a major pharmacologically active flavone from Artemisia, has been previously reported act as potent inducer dedifferentiation in liver lung. However, effects eupatilin on fibrosis have not yet investigated....

Abstract Profilin-1 (PFN1) regulates actin polymerization and cytoskeletal growth. Despite the essential roles of PFN1 in cell integration, its subcellular function keratinocyte has not been elucidated yet. Here we characterize specific regulation DNA damage response repair machinery. depletion accelerated damage-mediated apoptosis exhibiting PTEN loss instigated by increased phosphorylated inactivation followed high levels AKT activation. changed predominant cytoplasmic localization to...

Endometrial angiogenesis plays crucial roles in determining the endometrial receptivity. Defects receptivity often cause repeated implantation failure, which is one of major unmet needs for infertility and contributes a barrier to assisted reproductive technology. Despite numerous extensive research work, there are currently no effective evidence-based treatments prevent or cure this condition. As non-invasive treatment strategy, botulinum toxin A (BoTA) was administered into side mouse...

The emerging role of microglia in neurological disorders requires a novel method for obtaining massive amounts adult microglia. We aim to develop new bankable and expandable adult-like mice.The head neuroepithelial layer (NEL) that composed microglial progenitor cells at mouse E13.5 was dissected then cultured or banked. Microglia (MG) isolated from the NEL by magnetic-activated cell sorting system were obtained named NEL-MG.The included progenitors proliferate ramify over time with as...

We aimed to assess the efficacy of accumulated embryo transfer (ACC-ET) through several controlled ovarian hyperstimulation (COS) cycles increase rates pregnancy in patients with poor response (POR). retrospectively reviewed medical records 588 POR under 43-years old who underwent from January 2010 December 2015. compared rate (PR), clinical (CPR), and live birth (LBR) between ACC-ET (frozen-thawed: 47; fresh + frozen-thawed: 24) group (n = 71) ET groups 517). Characteristics were similar...

Despite extensive research on implantation failure, little is known about the molecular mechanisms underlying crosstalk between embryo and maternal endometrium, which critical for successful pregnancy. Profilin 1 (PFN1), expressed both in endometrial epithelium, acts as a potent regulator of actin polymerization cytoskeletal network. In this study, we identified specific role PFN1 during implantation.Morphological alterations depending status expression were assessed PFN1-depleted or control...