A5051020425- Heat shock proteins research
- Endoplasmic Reticulum Stress and Disease
- Insect Resistance and Genetics
- Viral Infectious Diseases and Gene Expression in Insects
- Natural Compound Pharmacology Studies
- Insect and Pesticide Research
- Toxin Mechanisms and Immunotoxins
- X-ray Diffraction in Crystallography
- Microtubule and mitosis dynamics
- Carbohydrate Chemistry and Synthesis
- Ubiquitin and proteasome pathways
- Enzyme Structure and Function
- Plant biochemistry and biosynthesis
- Crystallization and Solubility Studies
- Fungal and yeast genetics research
- Insect behavior and control techniques
- Insect symbiosis and bacterial influences
- Research on scale insects
- Retirement, Disability, and Employment
- ATP Synthase and ATPases Research
- Bacterial Genetics and Biotechnology
- Smoking Behavior and Cessation
- Natural product bioactivities and synthesis
- Protein Structure and Dynamics
- Luminescence and Fluorescent Materials
Arcadia University
2015-2023
University College London
2022
City College of New York
2010-2013
Icahn School of Medicine at Mount Sinai
2006-2007
University of Patras
2003-2007
University of California, San Diego
2001
Quality control systems facilitate polypeptide folding and degradation to maintain protein homeostasis. Molecular chaperones promote folding, whereas the ubiquitin/proteasome system mediates degradation. We show here that Saccharomyces cerevisiae Ubr1 Ubr2 ubiquitin ligases of unfolded or misfolded cytosolic polypeptides. also catalyzes ubiquitinylation denatured but not native luciferase in a purified system. This activity is based on direct interaction with Ubr1, although Hsp70 stimulates...
// Maria A. Theodoraki 1 , Celso O. Rezende Jr. 2 Oraphin Chantarasriwong 2, 3 Adriana D. Corben 4 Emmanuel Theodorakis and Mary L. Alpaugh 5 Department of Biology, Arcadia University, Philadelphia, PA, USA Chemistry Biochemistry, University California – San Diego, La Jolla, CA, Chemistry, Faculty Science, King Mongkut's Technology Thonburi, Bangkok, Thailand Pathology, Memorial Sloan Kettering Cancer Center, New York, NY, Surgery, Correspondence to: Alpaugh, e-mail: alpaughm@mskcc.org...
Smoking prevalence in several high-income countries is steadily declining but remains persistently high 'lower' socioeconomic position (SEP) groups, contributing to inequities morbidity and mortality. relieve stress a commonly endorsed motive for continued smoking; however, it unclear whether smoking has negative impact on motivation stop future quit attempts if so, associations are moderated by SEP. This was an observational study with cross-sectional prospective survey data from the...
Heat shock protein 70 (Hsp70) plays a central role in homeostasis and quality control conjunction with other chaperone machines, including Hsp90. The Hsp110 Sse1 promotes Hsp90 activity yeast, functions as nucleotide exchange factor (NEF) for cytosolic Hsp70, but the precise roles client maturation through Hsp70–Hsp90 system are not fully understood. We find that upon pharmacological inhibition of Hsp90, model kinase, Ste11ΔN, is rapidly degraded, whereas heterologously expressed...
Abstract This report presents the cDNA cloning, heat shock regulation and developmental expression of hsp90 gene homologue Mediterranean fruit fly Ceratitis capitata (medfly). The isolated contained coding region, 3′UTR most 5′UTR medfly homologue, which was named Cchsp83 . deduced CcHSP83 polypeptide all highly conserved amino acid segments that characterize cytosolic members HSP90 family. Genomic analysis showed is unique mapped at 94C division sixth polytene chromosome. size mRNA found to...
The 2nd International Symposium on the Chaperone Code took place October 26-28, 2023 at Hilton Alexandria Old Town, VA, USA. event featured more than 100 attendees from ten countries and provided a dynamic platform for established researchers, emerging investigators, postdoctoral fellows, students to share insights ideas diverse facets of molecular chaperones with strong focus their regulation by post-translational modifications. format fostered discussions collaboration among participants....
Natural products of the caged Garcinia xanthones (CGX) family are characterized by a unique chemical structure, potent bioactivities and promising pharmacological profiles. We have developed Claisen/Diels–Alder reaction cascade that, in combination with Pd(0)-catalyzed reverse prenylation, provides rapid efficient access to CGX pharmacophore, represented structure cluvenone. To further explore this we synthesized various A-ring oxygenated analogues cluvenone evaluated their terms growth...
Abstract The promoter of the hsp70 gene Drosophila melanogaster has been widely used for expression foreign genes in other insects. It generally assumed that because this is highly conserved, its will function efficiently species. We report results a quantitative comparison activities medfly and D. hsp 70 promoters vivo transformed medflies. constructed lines containing lacZ reporter under control two by using Minos ‐mediated germ‐line transformation. activity each was evaluated 15...
Abstract Accurate protein quantitation is essential for many cellular mechanistic studies. Existing technology relies on extrinsic sample evaluation that requires significant volumes of as well addition assay-specific reagents and importantly, a terminal analysis. This study exploits the unique chemical features fluorescent molecular rotor fluctuates between twisted-to-untwisted states, with subsequent intensity increase in fluorescence depending environmental conditions (e.g., viscosity)....
The UBR1 ubiquitin ligase promotes degradation of proteins via the N-end rule and by another mechanism that detects a misfolded conformation. Although was shown recently to act on protein kinases whose misfolding promoted inhibition Hsp90, it unknown whether this targeted other client types chaperone. We analyzed role in nuclear receptors are classical clients Hsp90. Our results showed deletion impaired glucocorticoid receptor androgen but not estrogen α. These findings demonstrate...
Extracted from gamboge resin, gambogic acid (
Abstract In order to define the regulatory elements responsible for expression of medfly hsp83 ( Cchsp83 ) gene, we determined sequence a genomic region gene that included 3,536 bp upstream transcription initiation site, first untranslated exon 144 bp, 275‐bp intron, and 516 second coding exon. Structural analysis 5′ flanking revealed presence typical TATA box, 28 start seven putative heat shock (HSEs) further upstream. The mRNA was found contain extensive secondary structure in 126...
Ribosomal protein S21 (RpS21) belongs to a small group of ribosomal or ribosome-associated proteins. Mutations in the RpS21 gene cause dominant Minute and recessive lethal tumorous phenotypes Drosophila melanogaster. Studies several organisms suggest that is involved regulation synthesis cell growth. In this report, we used an RT-PCR fragment D. melanogaster mRNA clone cDNA from Mediterranean fruit fly, Ceratitis capitata. The isolated contained both 5' 3' untranslated regions, encoded...