A5077171756- Angiogenesis and VEGF in Cancer
- Cancer, Hypoxia, and Metabolism
- Veterinary Oncology Research
- Cancer Genomics and Diagnostics
- TGF-β signaling in diseases
- Cell Adhesion Molecules Research
- Cancer Cells and Metastasis
- Infectious Diseases and Mycology
- Cancer-related gene regulation
- Microbial infections and disease research
- Epigenetics and DNA Methylation
- Metabolism, Diabetes, and Cancer
- Barrier Structure and Function Studies
- Cancer Research and Treatments
- RNA modifications and cancer
- Genetic factors in colorectal cancer
- Tumors and Oncological Cases
- Virus-based gene therapy research
- Cellular Mechanics and Interactions
- Carcinogens and Genotoxicity Assessment
- Microtubule and mitosis dynamics
- Genetic and phenotypic traits in livestock
- Colorectal Cancer Treatments and Studies
- Protease and Inhibitor Mechanisms
- Selenium in Biological Systems
University of Guelph
2014-2024
Ontario Veterinary College
1998-2015
The Research Council
2014
Sultan Qaboos University
2014
McMaster University
2004
Scripps Research Institute
2004
Western University
1991
University of Toronto
1985-1990
Centre for Disability Prevention and Rehabilitation
1988
The p53 tumor suppressor gene is inactivated in the majority of human cancers. Tumor cells deficient display a diminished rate apoptosis under hypoxic conditions, circumstance that might reduce their reliance on vascular supply, and hence responsiveness to antiangiogenic therapy. Here, we report mice bearing tumors derived from −/− HCT116 colorectal cancer were less responsive combination therapy than isogenic +/+ tumors. Thus, although targets genetically stable endothelial vasculature,...
AbstractIn cancer the extensive methylation found in bulk of chromatin is reduced, while normally unmethylated CpG islands become hypermethylated. Regions solid tumors are transiently and/or chronically exposed to ischemia (hypoxia) and reperfusion, conditions known contribute progression. We hypothesized that hypoxic microenvironment may influence local epigenetic alterations, leading inappropriate silencing re-awakening genes involved cancer. cultured human colorectal melanoma cell lines...
Histologic grading schemes for canine cutaneous mast cell tumors (MCTs) were not developed subcutaneous MCTs. Despite this, MCTs are currently categorized by many as grade II or higher. The aim of this investigation was to assess the pathology and clinical outcome provide a more accurate prognosis. Information on 306 dogs obtained from veterinarians correlated with histologic features. Mean median follow-up 842 891 days, respectively (range, 3–2,305 days). Only 27 (9%) confirmed cell–related...
Vascular endothelial growth factor (VEGF) is a potent mitogen and cytoprotective for vascular cells. Although VEGF ubiquitously expressed, its role in nonvascular tissues poorly understood. interacts with various cell surface receptors to mediate cellular effects. It previously has been thought that the receptor Flk-1/KDR, main signaling receptor, was expressed exclusively by However, present study using bovine rodent models, we demonstrate Flk-1/KDR are coexpressed ovarian granulosa mRNA...
Most re-endothelialization requires both cell migration and proliferation. To study the association between initiation of DNA synthesis in an vitro wound model, confluent cultures porcine aortic endothelial cells grown on glass coverslips were scraped to remove half monolayer. Treatment with 1 ng/ml taxol, a microtubule stabilizing drug, for 24 hours resulted no visible change F-actin or organization as assessed by fluorescence immunofluorescence microscopy. There was, however, reduction...
Molecular assays are widely used to prognosticate canine cutaneous mast cell tumors (MCT). There is limited information about these prognostic on MCT that arise in the subcutis. The aims of this study were evaluate utility KIT immunohistochemical labeling pattern, c- mutational status (presence internal tandem duplications exon 11), and proliferation markers—including mitotic index, Ki67, argyrophilic nucleolar organizing regions (AgNOR)—as independent markers for local recurrence and/or...
Folate and its synthetic form, folic acid (FA), are essential vitamins for the regeneration of S-adenosyl methionine molecules, thereby maintaining adequate cellular methylation. The deregulation DNA methylation is a contributing factor to carcinogenesis, as alterations in genetic may contribute stem cell reprogramming dedifferentiation processes that lead cancer (CSC) phenotype. Here, we investigate potential effects FA exposure on colonosphere formation cultured human colorectal (CRC)...
Previous reports showed that the Steroidal Glycoalkaloid Solamargine inhibited proliferation of non-melanoma skin cancer cells. However, was not tested systematically on different types melanoma cells and simultaneously normal either. In this study we aimed to investigate effect mechanism involved in inhibiting growth cells.Solamargine another three cell lines. Vertical phase metastatic primary lines WM239 WM115, respectively radial benign WM35 were used. The half inhibitory concentration...
Cancer is the leading cause of death in dogs, yet there are no established screening paradigms for early detection. Liquid biopsy methods that interrogate cancer-derived genomic alterations cell-free DNA blood being adopted multi-cancer detection human medicine and now available veterinary use. The CANcer Detection Dogs (CANDiD) study an international, multi-center clinical designed to validate performance a novel "liquid biopsy" test developed noninvasive characterization cancer dogs using...
Epithelial ovarian carcinoma (EOC) patients are usually diagnosed at an advanced stage, characterized by interperitoneal carcinomatosis and production of large volumes ascites. Vascular endothelial growth factor-A (VEGF-A) its main signaling receptor VEGFR2 (KDR) coexpressed in primary tumors, ascitic cells metastases, suggesting the existence autocrine VEGF-A/KDR loop EOC cells. In present study, we examined this possibility explored role protecting from apoptosis under anchorage free...
Abstract In this study the three‐dimensional organization of pinocytotic vesicles in mouse endothelia from permeable (choroid plexus, area postrema, and skeletal muscle) blood‐brain barrier (bbb) (cerebral gray white matter) microvessels was examined. Reconstructions 75 segments endothelial cells were done with very thin (<23 nm) serial sections tracings. A total 2,013 five tissues reconstructed for study. Vesicles classified as to whether they attached other (fused), connected golgi or...
Epigenetic modifications are involved in the initiation and progression of cancer. Expression patterns activity DNA methyltransferases (DNMTs) strictly controlled normal cells, however, regulation these enzymes is lost cancer cells due to unknown reasons. Cancer therapies which target DNMTs promising treatments hematologic cancers, but they lack effectiveness solid tumors. Solid tumors exhibit areas hypoxia hypoglycaemia their irregular dysfunctional vasculature, we previously showed that...
Receptors for the angiogenic factor VEGF are expressed by tumor cancer cells including melanoma, although their functionality remains unclear. Paired human melanoma cell lines WM115 and WM239 were used to investigate differences in expression of VEGFR2 vitro vivo with anti-VEGF antibody bevacizumab. Both VEGFR2, levels which modulated hypoxia. Detection native phosphorylated subcellular fractions under serum-free conditions showed presence a functional autocrine as well intracrine...
Sprouting angiogenesis requires vascular endothelial proliferation, migration and morphogenesis. The process is regulated by soluble factors, principally growth factor (VEGF), via bidirectional signaling through the Jagged/Notch system, leading to assignment of tip cell stalk identity. cytokine transforming beta (TGF-β) can either stimulate or inhibit its differential surface receptor signaling. Here we evaluate changes in expression angiogenic receptors when bovine aortic cells were exposed...
The provision of feed is a major cost in beef production. Therefore, the improvement efficiency warranted. direct assessment has limitations and alternatives are needed. Small intestine micro-architecture associated with function may be related to efficiency. objective was verify potential histomorphological differences small animals divergent From population 45 feedlot steers, 12 were selected low-RFI (superior efficiency) high-RFI (inferior at end finishing period. processed 13.79 ± 1.21...
Abstract Background The lymphatic system complements the blood circulatory in absorption and transport of nutrients, maintenance homeostasis. Angiopoietins 1 2 (Ang1 Ang2) are regulators both angiogenesis lymphangiogenesis through Tek/Tie-2 receptor tyrosine kinase. response endothelial cells to stimulation with either Ang1 or Ang2 is thought be dependent upon origin cells. In this study, we examined effects angiopoietins on lymphatic, venous arterial primary (bmLEC, bmVEC bmAEC,...
The decline in mammary epithelial cell number as lactation progresses may be due, part, to oxidative stress. Selenium is an integral component of several antioxidant enzymes. present study was conducted examine the effect stress and selenomethionine (SeMet) on morphology, viability, apoptosis, proliferation bovine cells (BMEC) primary culture. Cells were isolated from glands lactating dairy cows grown for 3 d a low-serum gel system containing lactogenic hormones 0 or 100 μM H2O2 with 0, 10,...