A5028008351- Autoimmune and Inflammatory Disorders Research
- Immune Cell Function and Interaction
- Streptococcal Infections and Treatments
- Adolescent and Pediatric Healthcare
- Inflammasome and immune disorders
- Systemic Lupus Erythematosus Research
- Infective Endocarditis Diagnosis and Management
- IL-33, ST2, and ILC Pathways
- Extracellular vesicles in disease
- Reproductive System and Pregnancy
- Neurogenetic and Muscular Disorders Research
- Study of Mite Species
- Allergic Rhinitis and Sensitization
- MicroRNA in disease regulation
- Parvovirus B19 Infection Studies
- Emergency and Acute Care Studies
- Dermatological diseases and infestations
- Respiratory viral infections research
- Kawasaki Disease and Coronary Complications
- Bird parasitology and diseases
- Medical Imaging and Pathology Studies
- Prenatal Substance Exposure Effects
- Plant Reproductive Biology
- Inflammatory Myopathies and Dermatomyositis
- RNA and protein synthesis mechanisms
Cincinnati Children's Hospital Medical Center
2015-2024
Fairfield Hospital
2022
University of Cincinnati Medical Center
2021
University of Cincinnati
2009-2019
Children’s Institute
2018
Foundation for the National Institutes of Health
2013
Children's Hospital of Pittsburgh
2013
University of Pittsburgh
2013
Johns Hopkins Children's Center
2013
Johns Hopkins University
2013
Objective Macrophage activation syndrome (MAS), a life‐threatening complication of systemic juvenile idiopathic arthritis (JIA), resembles familial hemophagocytic lymphohistiocytosis (HLH), constellation autosomal‐recessive immune disorders resulting from deficiency in cytolytic pathway proteins. We undertook this study to test our hypothesis that MAS predisposition JIA could be attributed rare gene sequence variants affecting the cytotolytic pathway. Methods Whole‐exome sequencing was used...
Abstract Objective Systemic juvenile idiopathic arthritis (JIA) is frequently associated with the development of macrophage activation syndrome. This study was undertaken to better understand relationship between systemic JIA and Methods Gene expression profiles were examined in 17 patients untreated new‐onset JIA, 5 whom showed evidence subclinical syndrome (of 2 eventually developed overt syndrome). Peripheral blood mononuclear cells (PBMCs) separated on Ficoll gradients, purified RNA...
Abstract Objective To identify differences in peripheral blood gene expression between patients with different subclasses of juvenile idiopathic arthritis (JIA) and healthy controls a multicenter study recent‐onset JIA prior to treatment disease‐modifying antirheumatic drugs (DMARDs) or biologic agents. Methods Peripheral mononuclear cells (PBMCs) from 59 children 136 (28 enthesitis‐related [ERA], 42 persistent oligoarthritis, 45 rheumatoid factor [RF]–negative polyarthritis, 21 systemic...
Background. Severe H1N1 influenza can be lethal in otherwise healthy individuals and have features of reactive hemophagocytic lymphohistiocytosis (HLH). HLH is associated with mutations lymphocyte cytolytic pathway genes, which not been previously explored influenza.
Abstract Objectives Systemic juvenile idiopathic arthritis (sJIA) is a childhood with features of autoinflammation and high risk macrophage activation syndrome (MAS). IL-18 has been shown to have key roles in sJIA MAS. We aimed examine levels relation disease activity history MAS other biomarkers namely S100 proteins CXCL9. Methods Total IL-18, CXCL9 were determined 40 patients, compared between patients regards activity, MAS, biomarkers. Results significantly higher active (median 16 499...
Objective Systemic‐onset juvenile idiopathic arthritis (JIA) is speculated to follow a biphasic course, with an initial systemic disease phase driven by innate immune mechanisms and interleukin‐1β (IL‐1β) as key cytokine second chronic arthritic that may be dominated adaptive immunity cytokines such IL‐17A. Although recent mouse model points critical role of IL‐17–expressing γ/δ T cells in pathology, humans, both the prevalence IL‐17 IL‐17–producing are still unclear. Methods Serum samples...
Objective Systemic juvenile idiopathic arthritis (JIA) is an inflammatory disease of childhood in which cells the monomyelocytoid lineage are thought to be key effector cells. Monocytes from patients with systemic JIA have a distinct phenotype, features both M1 and M2 alternative activation. MicroRNAs critical regulators monocyte polarization function, but cellular microRNAs not been examined systematically. Methods MicroRNA TaqMan arrays were used determine expression profiles monocytes...
Objective. Follistatin-like protein 1 (FSTL-1) is a secreted glycoprotein overexpressed in certain inflammatory diseases. Our objective was to correlate FSTL-1 levels with gene expression, known biomarkers, and measures of disease activity systemic juvenile idiopathic arthritis (sJIA), including macrophage activation syndrome (MAS). Methods. serum were measured by ELISA 28 patients sJIA, 7 who developed MAS, 30 healthy controls. Levels correlated erythrocyte sedimentation rate (ESR),...
Improved, noninvasive biomarkers are needed to accurately detect lupus nephritis (LN) activity. The purpose of this study was evaluate five S100 proteins (S100A4, S100A6, S100A8/9, and S100A12) in both serum urine as potential global renal system-specific disease activity childhood-onset systemic erythematosus (cSLE). In multicenter study, were measured the four cSLE cohorts healthy control subjects using commercial enzyme-linked immunosorbent assays. Patients divided into on basis...
Systemic juvenile idiopathic arthritis (SJIA) confers high risk for macrophage activation syndrome (MAS), a life-threatening cytokine storm driven by interferon (IFN)-γ. SJIA monocytes display IFN-γ hyper-responsiveness, but the molecular basis of this remains unclear. The objective study is to identify circulating monocyte and bone marrow (BMM) polarisation phenotypes in including features contributing IFN response.Bulk RNA-seq was performed on peripheral blood (n=26 patients) single cell...
Abstract Introduction Previous observations suggest that active systemic juvenile idiopathic arthritis (sJIA) is associated with a prominent erythropoiesis gene-expression signature. The aim of this study was to determine the association signature peripheral blood mononuclear cell (PBMC) subpopulations and its specificity for sJIA as compared related conditions. Methods 199 patients JIA (23 176 non-sJIA) 38 controls were studied. PBMCs isolated analyzed multiple surface antigens flow...
Objective Macrophage activation syndrome (MAS) is a life‐threatening complication of systemic juvenile idiopathic arthritis (JIA) and has pathologic similarity to hemophagocytic lymphohistiocytosis (HLH). Intronic variants in UNC13D are found patients with familial HLH type 3 (FHLH3), but the role noncoding MAS unknown. The objective this study was identify deep intronic MAS. Methods A custom enrichment library constructed sequence genomic region ~1 Mb flanking 24 JIA, recurrent MAS,...
Biomarkers in easily obtained specimens that accurately predict uveitis children with juvenile idiopathic arthritis (JIA) are needed. Aqueous humor has been studied for biomarkers, but is not routinely available. We evaluated tears from chronic anterior (CAU) biomarkers reported aqueous humor. In this pilot study, we used Schirmer strips to collect seven (nine eyes); three had JIA- associated (JIA-U) and four disease (I-CAU). Liquid chromatography-tandem mass spectrometry was identify...
Abstract Objective To investigate the relationship between vasculopathy of juvenile dermatomyositis (juvenile DM) and balance angiostatic ELR− angiogenic ELR+ CXC chemokines in muscle patients with disease. Methods The expression 3 (interferon‐inducible protein 10 [IP‐10], monokine induced by interferon‐γ, interferon‐inducible T cell α‐chemoattractant) 2 was quantitated biopsy samples from 7 DM healthy children, real‐time polymerase chain reaction. findings were correlated various...
Abstract Objective Systemic juvenile idiopathic arthritis (JIA) is an autoinflammatory syndrome in which the myelomonocytic lineage appears to play a pivotal role. Inflammatory macrophages are driven by interferon‐γ (IFNγ), but studies have failed demonstrate IFN‐ induced gene signature active systemic JIA. This study sought characterize status of IFN‐induced within affected tissue and gauge integrity IFN signaling pathways peripheral monocytes from patients with Methods Synovial 12 JIA 9...
Background The Active Community Case Management Platform is a cloud‐based technology developed to facilitate rheumatic heart disease case management by health care providers. This study aimed design and pilot an automated short message service (SMS) intervention support secondary prophylaxis adherence. Methods Results We concise library of messages antibiotic prophylaxis. SMS used TextIT, interface that enables users send out interactive at scale. bank was piloted in cohort 50 patients with...
The quality of RNA preserved in different stabilization matrices was investigated after 2 weeks storage at room temperature. samples RNAstable (Biomatrica), GenTegra (IntegenX), and RNAshell (Imagene) were compared to stored −80°C (the current gold standard for preservation) with liquid or dried temperature without additives this multi-center study. One center prepared all the samples, five participating laboratories applied them Samples shipped three testing laboratories, where 336...
The clinical signs of a Sarcoptes scabiei (De Geer) (Acari: Sarcoptidae) infestation are initially delayed, which suggests that the mites can depress immune/inflammatory response. purpose this study was to investigate modulatory properties scabies in vivo at gene expression level secondary lymphoid organ is involved initiating an immune response parasite. We found substances from influenced mRNA for molecules participate sequestering lymphocytes periarteriolar sheath, primary follicle, and...
MicroRNAs (miRNAs) are involved in the post-transcriptional regulation of genes. The objective this study was to investigate whether select urinary cell-free microRNA's may serve as biomarkers children with active lupus nephritis (LN) and assess their relationship recently identified combinatorial urine biomarkers, a.k.a. LN-Panel (neutrophil gelatinase associated lipocalin, monocyte chemotactic protein 1, transferrin, beta-trace protein). miRNAs (125a, 127, 146a, 150 155) were measured...
The clinical signs of a Sarcoptes scabiei (De Geer) (Acari: Sarcoptidae) infestation are initially delayed, which suggests that the mites can depress immune/inflammatory response. purpose this study was to investigate modulatory properties scabies in vivo at gene expression level secondary lymphoid organ is involved initiating an immune response parasite. We found substances from influenced mRNA for molecules participate sequestering lymphocytes periarteriolar sheath, primary follicle, and...
Abstract Objective Most rheumatic heart disease (RHD) registries are static and centralized, collecting epidemiological clinical data without providing tools to improve care. We developed a dynamic cloud-based RHD case management application with the goal of improving care for patients in Uganda. Methods The Active Community Case Management Tool (ACT) was designed community-based chronic disease, as first test case. Global local partner consultation informed selection critical fields...