Background/Objectives: This research aimed to determine the efficacy of metallic oxide nanoparticles, especially zinc nanoparticles (ZnO-NPs), in inhibiting a wide range bacteria isolated from animal wounds, indicating their potential as alternative antimicrobial therapies veterinary medicine. Method: The disc diffusion technique, broth microdilution and time-kill kinetic assay were performed antibacterial activity ZnO-NPs. Results: Transmission electron microscopy (TEM) scanning (SEM showed...

Traditionally, non-aureus staphylococci and mammaliicocci (NASM) were not considered significant players in bovine mastitis. This study investigated the involvement of NASM (Staphylococcus hominis Staphylococcus chromogenes) lactic acid bacteria (LAB) strains (Weissella paramesenteroides) through neutrophil responses. Bovine neutrophils displayed minimal apoptosis upon LAB challenge. Neutrophils expressed high TLR2 after challenge, but TLR6 expression varied remained low pathogen...

Streptococcus uberis is frequently isolated from milk collected dairy cows with mastitis. According to the host’s immunity, bacterial virulence, and their interaction, infection some strains can induce persistent subclinical inflammation, while others induces severe inflammation transient This study compared inflammatory response of milk-isolated white blood cells (mWBCs) S . strains. Quarter samples were aseptically for culture all lactating once a week over 10-week period. A noncapsular...

The present study evaluated the antimicrobial activities of acetic acid against bovine mastitis pathogens compared to lactic and lauric caprylic saturated fatty acids. Eleven were isolated from sub-clinical clinical cases for study. An initial screening their antibacterial by agar well diffusion method was performed. minimum inhibitory concentration (MIC) bactericidal (MBC) each obtained using a microdilution method; diluted stock solution then with culture broth reach concentrations ranging...

The use of metal oxide nanoparticles as an alternative antimicrobial agent has gained attention due to the increasing problem resistance. Understanding its properties and potential benefits can contribute development more effective sustainable treatments in veterinary medicine. aim this study was characterize TiO2-NP formulations evaluate their antibacterial wound healing abilities. diameters zeta potentials were determined using Zetasizer conjunction with dynamic light scattering. agar-well...

Poor mastitis control favors intramammary infection (IMI), which always involves CNS. This study aimed to determine the relationships of IL-4, IL-6, and IL-10 in milk with concurrent infection, bacterial pathogens, SCC, MDA, an oxidative stress marker. All quarters from five smallholder dairy farms were sampled aseptically before morning milking again afternoon for bacteriological identification using MALDI-TOF mass spectrometry. The samples concomitant between streptococci CNS their pairs...

Herbal phytochemicals featuring active ingredients including quercetin and curcumin have shown potential in treating human animal diseases. The current study investigated their function vitro for host immunomodulation associated with

The objectives of this study were determined by two experiments including Experiment 1 (EXP1) using Streptococcus uberis obtained from a weekly longitudinal to compare virulence patterns between transient and persistent intramammary infection (IMI), 2 (EXP2) stored-known-appearance PFGE strain contagious S. determine change after long-term transmission. For EXP1, quarter milk samples 31 milking cows aseptically longitudinally collected once week for 10 weeks. A total 14 isolates quarters...

The aim of this study was to determine the screening for antimicrobial properties against mastitis pathogens turmeric extract in combination with various antiseptics. antibacterial effects antiseptics (5% povidone iodine, 0.5% v/v hydrogen peroxide (H2O2), chlorine (Cl2), and chlorhexidine) were determined using agar welldiffusion method. Results showed that additional had significantly decreased activities either Cl2or chlorhexidine almost all pathogens, except S. agalactiaefor Cl2 gram...

The microbial ecology in mastitis involves the interactions between bacteria and mammary gland environment. Poor control, for which understanding these relationships is crucial, increases risk of co-infections. aim this study was to determine pathogenesis bacterial murine glands following intramammary infection (IMI) with

The use of metal oxide nanoparticles as an alternative antimicrobial agent has gained attention due to the increasing problem antibiotic resistance. Understanding its properties and potential benefits can contribute development more effective sustainable treatments in veterinary medicine. aim this study was characterize TiO2-NP formulations evaluate their antibacterial wound healing abilities. diameters zeta potentials were determined using Zetasizer conjunction with dynamic light...

The objective of this study was to determine the antibacterial properties lauric acid in combination with acetic and lactic against major dairy mastitis pathogens including Staphylococcus aureus, Streptococcus agalactiae, uberis, Escherichia coli Klebsiella spp. effect each mixtures evaluated by their minimum inhibitory concentration (MIC) bactericidal concentrations (MBC) using broth microdilution method. differences in, MIC MBC values for pathogen were calculated applying one-way analysis...

Background and Aim: Mastitis, primarily caused by intramammary bacterial infection, is the most expensive disease in global dairy industry due to its negative impact on milk composition manufacturing properties. This study aimed evaluate efficacy of parenteral amoxicillin treatment clinical subclinical mastitis smallholder farms Northern Thailand. Materials Methods: A total 51 cows with from cooperatives Lamphun Chiang Mai provinces, Thailand, were enrolled this study. Conventional...