Bovine mastitis, an inflammatory condition of the mammary gland, is a critical economic issue in dairy industry. Antimicrobial resistance (AMR) to mastitis-causing pathogens poses significant threat operations Thailand and Cambodia. This study investigates AMR mastitis It focuses on detecting methicillin-resistant Staphylococcus aureus, vancomycin-resistant S. aureus (VRSA), enterococci by identifying presence mecA, vanA, vanB genes bacterial isolates. A total 65 isolates (55 from 10...

Background/Objectives: This research aimed to determine the efficacy of metallic oxide nanoparticles, especially zinc nanoparticles (ZnO-NPs), in inhibiting a wide range bacteria isolated from animal wounds, indicating their potential as alternative antimicrobial therapies veterinary medicine. Method: The disc diffusion technique, broth microdilution and time-kill kinetic assay were performed antibacterial activity ZnO-NPs. Results: Transmission electron microscopy (TEM) scanning (SEM showed...

The complete mastitis control program is insufficient for the starting dairy industry country, and therefore it might cause emerging of new pathogens. This longitudinal study aimed to determine association infected dynamic status main pathogens responsible with seasonal variations, proportions transient chronic intramammary infection (IMI) episodes, duration IMI. was conducted on a training smallholder farm in Phnom Penh, Cambodia, from January 2023 July 2023. Trained veterinarians...

Fischoederius elongatus (Poirier, 1883) is a common rumen fluke found in Asia and Africa. Nucleotide sequence data for F. have been reported but are limited to certain ribosomal mitochondrial DNA regions. High diversity the nucleotide sequences of cytochrome c oxidase subunit 1 (COI) haplotypes has some Asian countries. Some researchers advocated possibility cryptic species within based on high genetic as well distance between sister species. However, number examined parasites, hosts,...

Traditionally, non-aureus staphylococci and mammaliicocci (NASM) were not considered significant players in bovine mastitis. This study investigated the involvement of NASM (Staphylococcus hominis Staphylococcus chromogenes) lactic acid bacteria (LAB) strains (Weissella paramesenteroides) through neutrophil responses. Bovine neutrophils displayed minimal apoptosis upon LAB challenge. Neutrophils expressed high TLR2 after challenge, but TLR6 expression varied remained low pathogen...

Dermatophytosis is a disease caused by dermatophytes, group of fungi that can cause both in humans and animals. The important genera are pathogenic animals include Trichophyton Microsporum. Microsporum canis an species because it zoonosis commonly found domestic Cats, which live very close to humans, may expose this pathogen. This research focused on the epidemiology M. cats. Hair samples were collected via Mackenzie technique from cats with without skin lesions, preliminarily examined 10%...

Abstract: A cross‐sectional survey was designed to investigate the proportion of tetracycline residues in marketed pork suburb and urban districts Hanoi. total 290 raw muscle samples were randomly collected from open markets these districts. The qualitatively screened for using agar inhibition test, Bacillus cereus (ATCC 11778) as reference strain. inconclusive then analyzed high‐performance liquid chromatography (HPLC). positive either test defined results. Overall, 5.5% all residues. shops...

Antibiotic resistance patterns of bacterial isolates from both quarter teat-tip swabs and their milk samples were evaluated in smallholder dairy farms northern Thailand with excessive use antibiotics (HIGH) compared normal (NORM). Results swab showed that the percentage Bacillus spp. to overall was significantly lower NORM group than HIGH group, whereas, coagulase-negative staphylococci higher one. The mastitis-causing bacteria isolated environmental streptococci (13.8%), (9.9%),...

The objective of this study was to determine cow characteristics and farm management factors during the dry period associated with early postparturient intramammary infection (IMI) subsequent clinical mastitis (CM). Data were collected three times: before drying off (P1), (P2), 5 14 days after calving (P3), using questionnaires investigation. Milk samples aseptically for bacterial identification at P1 P3. Factors IMI CM analyzed multiple logistic regression models. final model showed that in...

To obtain yeast and bacteria from ruminal fluids that possess aflatoxin B

Streptococcus uberis is frequently isolated from milk collected dairy cows with mastitis. According to the host’s immunity, bacterial virulence, and their interaction, infection some strains can induce persistent subclinical inflammation, while others induces severe inflammation transient This study compared inflammatory response of milk-isolated white blood cells (mWBCs) S . strains. Quarter samples were aseptically for culture all lactating once a week over 10-week period. A noncapsular...

Aflatoxin M1 (AFM1) levels were determined in pasteurised milk from five commercial trademarks produced different areas Thailand. One hundred and twenty samples collected local markets Chiang Mai province, Thailand, to evaluate AFM1 concentrations using immunoaffinity columns high-performance liquid chromatography with fluorescence detection. The overall median level was 0.023 µg L(-1) ranging 0.004 0.293 L(-1). All had average lower than 0.05 L(-1), those Trademarks 3 5 being higher 1 2 (P...

The objective of this study was to determine the effect supplementing Kluyveromyces marxianus CPY1, K. RSY5 and Pichia kudriavzevii YSY2 isolated from ruminal fluid dairy cows on transfer aflatoxin B1 (AFB1) feed into M1 (AFM1) in milk, DMI, milk production nutrient digestibility. Four multiparous Holsteins mid-lactation were used a 4 × Latin square design trial consisting 14 days each experimental period for sample collection. Between period, clearance prior next treatment allowed minimize...

The present study evaluated the antimicrobial activities of acetic acid against bovine mastitis pathogens compared to lactic and lauric caprylic saturated fatty acids. Eleven were isolated from sub-clinical clinical cases for study. An initial screening their antibacterial by agar well diffusion method was performed. minimum inhibitory concentration (MIC) bactericidal (MBC) each obtained using a microdilution method; diluted stock solution then with culture broth reach concentrations ranging...

Bovine mastitis caused by Staphylococcus aureus may exacerbate resulting in significant economic losses and impacting milk quality. To date, the use of gallic acid, a phenolic compound naturally occurring various plants, holds promise due to its potent anti-oxidant anti-inflammatory effects many pieces literature, thus, making it subject interest bovine innate immunity research. Here we used acid assess potential immunomodulation on phagocytes vitro challenges with mastitis-causing bacteria....

Bovine mastitis is primarily caused by a group of bacteria known as Staphylococcus and Streptococcus. However, additional types bacteria, such bovine non-aureus staphylococci mammaliicocci (NASM) well lactic acid (LAB), are considered minor pathogens have less impact on cows. Modulating neutrophil activities gene expressions in response to bacterial stimuli prompted the cells execute effector functions combat udder infections. Although neutrophils can manage major mastitis-causing this...

The use of metal oxide nanoparticles as an alternative antimicrobial agent has gained attention due to the increasing problem resistance. Understanding its properties and potential benefits can contribute development more effective sustainable treatments in veterinary medicine. aim this study was characterize TiO2-NP formulations evaluate their antibacterial wound healing abilities. diameters zeta potentials were determined using Zetasizer conjunction with dynamic light scattering. agar-well...