A5012969114- Chemokine receptors and signaling
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- CRISPR and Genetic Engineering
- T-cell and B-cell Immunology
- Diabetes and associated disorders
- Molecular Biology Techniques and Applications
- Genomics and Chromatin Dynamics
- interferon and immune responses
- DNA Repair Mechanisms
- Telomeres, Telomerase, and Senescence
- Cancer-related molecular mechanisms research
- IL-33, ST2, and ILC Pathways
- Ferroptosis and cancer prognosis
- Hepatocellular Carcinoma Treatment and Prognosis
- Cardiac Imaging and Diagnostics
- Cytokine Signaling Pathways and Interactions
- Adrenal Hormones and Disorders
- Cancer, Lipids, and Metabolism
- Liver Disease Diagnosis and Treatment
- RNA and protein synthesis mechanisms
- Pancreatic function and diabetes
- Hepatitis C virus research
- Eosinophilic Esophagitis
- Glycosylation and Glycoproteins Research
Memorial Sloan Kettering Cancer Center
2020-2025
Icahn School of Medicine at Mount Sinai
2004-2017
Tisch Hospital
2015
Scripps Green Hospital
2014
Brown University
2014
Rhode Island Hospital
2014
Hokkaido University
2014
Keimyung University
2014
Abstract Background & Aims Hepatocellular carcinoma ( HCC ) is the second most lethal cancer caused by lack of effective therapies. Although promising, molecular classification, which enriches potential responders to specific therapies, has not yet been assessed in clinical trials anti‐ drugs. We aimed overcome these challenges developing clinicopathological surrogate indices classification. Methods classification defined our previous transcriptome meta‐analysis (S1, S2 and S3...
<h3>Objective</h3> The number of patients with HCV-related cirrhosis is increasing, leading to a rising risk complications and death. Prognostic stratification in early-stage still challenging. We aimed develop validate clinically useful prognostic index based on genomic clinical variables identify at high disease progression. <h3>Design</h3> developed index, comprised 186-gene signature validated our previous genome-wide profiling study, bilirubin (>1 mg/dL) platelet count (<100...
Increased expression of Interleukin (IL)-33 has been detected in intestinal samples patients with ulcerative colitis, a condition associated increased risk for colon cancer, but its role the development colorectal cancer yet to be fully examined. Here, we investigated epithelial expressed IL-33 during tumors. was cells specimens and Apc Min/+ mice. To better understand epithelial-derived tumorigenesis, generated transgenic mice expressing (V33 mice). V33 mice, resulting from cross had tumor...
Abstract Telomere repeat containing RNAs (TERRAs) are a family of long non-coding transcribed from the subtelomeric regions eukaryotic chromosomes. TERRA transcripts can form R-loops at chromosome ends; however importance these structures or regulation expression and retention in telomeric remain unclear. Here, we show that RTEL1 (Regulator Length 1) helicase influences abundance localization human cells. Depletion leads to increased levels RNA while reducing TERRA-containing R loops...
Abstract Lymphocytic infiltrates and lymphoid follicles with germinal centers are often detected in autoimmune thyroid disease (AITD), but the mechanisms underlying lymphocyte entry organization remain unknown. We tested hypothesis that CCL21, a chemokine regulates homeostatic trafficking, whose expression has been AITD, is involved migration of lymphocytes to thyroid. show transgenic mice expressing CCL21 from thyroglobulin promoter (TGCCL21 mice) have significant lymphocytic infiltrates,...
D6, a promiscuous nonsignaling chemokine binding molecule expressed on the lymphatic endothelium, internalizes and degrades CC chemokines, D6(-/-) mice demonstrated increased cutaneous inflammation following topical phorbol ester or CFA injection. We report that were unexpectedly resistant to induction of experimental autoimmune encephalomyelitis due impaired encephalitogenic responses. Following with myelin oligodendroglial glycoprotein (MOG) peptide 35-55 in CFA, showed reduced spinal cord...
Background Archived tissues from previously completed prospective trials represent invaluable resource for biomarker development. However, such specimens are often stored as sections on glass slides, in which RNA is severely degraded due to prolonged air exposure. We evaluated whether a proportion of archived sectioned formalin-fixed paraffin-embedded (AS-FFPE) yield transcriptome profiles comparable freshly cut (FC) FFPE tissues, can be used retrospective class prediction analysis. Methods...
Abstract Nucleolytic resection of DNA ends is critical for homologous recombination, but its mechanism not fully understood, particularly in mammalian meiosis. Here we examine roles the conserved MRN complex (MRE11, RAD50, and NBS1) through genome-wide analysis meiotic during spermatogenesis mice with various mutations, including several that cause chromosomal instability humans. Meiotic DSBs form at elevated levels remain unresected if Mre11 conditionally deleted, thus required both...
Abstract Multiple injections of low-dose streptozotocin (MLDS) induce lymphocytic insulitis and diabetes in rodents. To test whether the influx inflammatory cells was associated with changes expression chemokines, we measured all known chemokine ligands by real-time quantitative PCR isolated islets. With exception CCL20 CCL19, chemokines were not significantly expressed islets from wild-type mice before MLDS treatment. Ten days after treatment, several including CXCL9, CCL1, CXCL10, CCL21,...
ABSTRACT Nucleolytic resection of DNA ends is critical for homologous recombination, but its mechanism not fully understood, particularly in mammalian meiosis. Here we examine roles the conserved MRN complex (MRE11, RAD50, and NBS1) through genome-wide analysis meiotic mice with various mutations, including several that cause chromosomal instability humans. Meiotic DSBs form at elevated levels remain unresected if Mre11 conditionally deleted, thus required both initiation regulation DSB...
CCR7 is involved in the initiation of immune responses and has been recently implicated control tolerance. To analyze role autoimmunity, we backcrossed CCR7(ko/ko) mice (in which ko signifies deficient) onto autoimmune-prone NOD background. Surprisingly, NODCCR7(ko/ko) never developed diabetes, but showed severe inflammation multiple tissues including thyroid, lung, stomach, intestine, uterus, testis. had a marked enlargement thyroid gland (goiter) that was associated with circulating...
Type 1 diabetes is an autoimmune disease characterized by a local inflammatory reaction in and around islets followed selective destruction of insulin-secreting beta-cells. We tested the hypothesis that chemokines affect different mechanisms responsible for development NOD mice.We examined chemokine expression mice their functional relevance to using transgenic expressing mouse herpesvirus 68-encoded decoy receptor M3 (NOD-M3 mice) beta-cells.Multiple were expressed pancreatic before...
Abstract Chemokines and their receptors play a key role in immune homeostasis regulating leukocyte migration, differentiation, function. Viruses have acquired optimized molecules that interact with the chemokine system. These virus-encoded promote cell entry, facilitate dissemination of infected cells, enable virus to evade response. One such molecule murine gammaherpesvirus 68 genome is M3 gene, which encodes secreted 44-kDa protein binds high affinity certain human chemokines blocks...
The MRE11 complex (comprising MRE11, RAD50, and NBS1) is integral to the maintenance of genome stability. We previously showed that a hypomorphic Mre11 mutant mouse strain ( ATLD1/ATLD1 ) was highly susceptible oncogene-induced breast cancer. Here we used mammary organoid system examine which MRE11-dependent responses are tumor-suppressive. found organoids exhibited an elevated interferon-stimulated gene (ISG) signature sustained changes in chromatin accessibility. This phenotype depended on...
The Mre11 complex (comprising Mre11, Rad50, Nbs1) is integral to the maintenance of genome stability. We previously showed that a hypomorphic
Summary Telomere repeat containing RNAs (TERRAs) are a family of long non-coding transcribed from the sub-telomeric regions eukaryotic chromosomes. TERRA transcripts can form R-loops at chromosome ends; however importance these structures or regulation expression and retention in telomeric remain unclear. Here, we show that RTEL1 (Regulator Length 1) helicase influences abundance localization human cells. Depletion leads to increased levels RNA while reducing TERRA-containing R loops...
BACKGROUND: Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related death worldwide.Over 600,000 new cases are diagnosed each year.Early stage disease can be cured with surgical resection or liver transplantation.Liver transplantation offers best chance at a cure; however, recurrence rates as high 40% for those transplanted.The enumeration circulating tumor cells (CTCs) an independent prognostic biomarker in patients malignancies including breast and colon cancer.CTCs...
Abstract D6, a promiscuous non-signaling chemokine binding molecule expressed on lymphatic endothelium, internalizes and degrades CC chemokines, D6−/− mice demonstrated increased cutaneous inflammation following topical phorbol ester or complete Freund’s adjuvant (CFA) injection. We report that were unexpectedly resistant to induction of experimental autoimmune encephalomyelitis (EAE), due impaired encephalitogenic responses. Following with myelin oligodendroglial glycoprotein (MOG) peptide...