A5044794996- Osteoarthritis Treatment and Mechanisms
- Knee injuries and reconstruction techniques
- Periodontal Regeneration and Treatments
- Mesenchymal stem cell research
- Total Knee Arthroplasty Outcomes
- Tendon Structure and Treatment
- Cancer-related molecular mechanisms research
- Tissue Engineering and Regenerative Medicine
- Bone Tissue Engineering Materials
- Lipid metabolism and disorders
- Medical and Biological Sciences
- Bone fractures and treatments
- Shoulder Injury and Treatment
- Bone health and treatments
- Bone Metabolism and Diseases
- Shoulder and Clavicle Injuries
- Angiogenesis and VEGF in Cancer
- Silk-based biomaterials and applications
- Hematopoietic Stem Cell Transplantation
- Electrospun Nanofibers in Biomedical Applications
- Lower Extremity Biomechanics and Pathologies
- dental development and anomalies
- Bone and Joint Diseases
- Management of metastatic bone disease
- Cancer Diagnosis and Treatment
Telethon Foundation
2024-2025
Novartis (Switzerland)
2012-2023
Novartis Institutes for BioMedical Research
2011-2021
Istituto Ortopedico Galeazzi
2009-2020
University Hospital of Basel
2009-2020
University of Basel
2014-2020
Istituti di Ricovero e Cura a Carattere Scientifico
2009-2018
Universidade de São Paulo
2018
University of California, San Diego
2015
Sanford Consortium for Regenerative Medicine
2015
Mesenchymal stem/stromal cells (MSC) are typically used to generate bone tissue by a process resembling intramembranous ossification, i.e., direct osteoblastic differentiation. However, most bones develop endochondral via remodeling of hypertrophic cartilaginous templates. To date, formation has not been reproduced using human, clinically compliant cell sources. Here, we aimed at engineering tissues from marrow-derived, adult human MSC with an intrinsic capacity undergo ossification. By...
Embryonic development, lengthening, and repair of most bones proceed by endochondral ossification, namely through formation a cartilage intermediate. It was previously demonstrated that adult human bone marrow-derived mesenchymal stem/stromal cells (hMSCs) can execute an program ectopically generate mature bone. Here we hypothesized hMSCs pushed ossification engineer scaled-up ossicle with features "bone organ," including physiologically remodeled bone, vasculature, fully functional...
Objective: The purpose of our study was to determine the effectiveness cartilage repair utilizing 1-step surgery with bone marrow aspirate concentrate (BMAC) and a collagen I/III matrix (Chondro-Gide, Geistlich, Wolhusen, Switzerland). Materials Methods: We prospectively followed up for 2 years 15 patients (mean age, 48 years) who were operated grade IV lesions knee. Six had multiple chondral lesions; average size 9.2 cm . All underwent mini-arthrotomy concomitant transplantation BMAC...
Rotator cuff repair typically results in a satisfactory, although variable, clinical outcome. However, anatomic failure of the repaired tendon often occurs.Patch augmentation can improve open rotator by supporting healing process, protecting suture, and reducing friction subacromial space.Cohort study; Level evidence, 3.A total 152 patients with posterosuperior massive tear were treated only (control group; n = 51; mean age, 67.06 ± 4.42 years), together collagen patch (collagen 49; 66.53...
The aim of this study is to prospectively evaluate the medium-term effectiveness and regenerative capability autologous adult mesenchymal stem cells, harvested as bone marrow aspirate concentrate (BMAC), along with a hyaluronan-based scaffold (Hyalofast) in treatment ICRS grade 4 chondral lesions knee joint, patients older than 45 years.A group 20 an age >45 years (mean 50.0 ± 4.1 years) was compared control <45 36.6 5.0). Patients were evaluated for years. All MRI, KOOS, IKDC, VAS Tegner...
Osteoarthritis is a common inflammatory disorder with no disease-modifying therapies. Whether inhibition of interleukin-1β (IL-1β) can reduce the consequences large joint osteoarthritis unclear.To determine whether IL-1β canakinumab reduces incident total hip or knee replacement (THR/TKR).Exploratory analysis randomized trial. (ClinicalTrials.gov: NCT01327846).1091 clinical sites in 39 countries.10 061 CANTOS (Canakinumab Anti-inflammatory Thrombosis Outcomes Study) participants.Random...
HOX -negative, differentiated neural crest–derived adult cells from the nasal septum display self-renewal capacity and environmental plasticity are compatible for articular cartilage repair.
Significance It has been previously reported that hypertrophic cartilage tissues engineered from human mesenchymal stromal cells can efficiently remodel in vivo into bone organs, recapitulating developmental steps of endochondral ossification. We have here demonstrated the extracellular matrix (ECM) such cartilage, even absence a living cell component, retains frankly osteoinductive properties. The use an apoptosis-driven devitalization technique revealed importance preserving ECM integrity...
Osteoarthritis (OA) is a common, debilitating, chronic disease with no disease-modifying drug approved to date. We discovered LNA043-a derivative of angiopoietin-like 3 (ANGPTL3)-as potent chondrogenesis inducer using phenotypic screen human mesenchymal stem cells. show that LNA043 promotes and cartilage matrix synthesis in vitro regenerates hyaline articular preclinical OA injury models vivo. exerts at least part these effects through binding the fibronectin receptor, integrin α5β1 on cells...
Inflammatory cytokines present in the milieu of fracture site are important modulators bone healing.Here we investigated effects interleukin-1β (IL-1β) on main events endochondral formation by human marrow mesenchymal stromal cells (BM-MSC), namely cell proliferation, differentiation and maturation/ remodelling resulting hypertrophic cartilage.Low doses IL-1β (50 pg/mL) enhanced colony-forming unitsfibroblastic (CFU-f) -osteoblastic (CFU-o) number (up to 1.5-fold) size (1.2-fold) absence...
Previous studies showed that human nasal chondrocytes (HNC) exhibit higher proliferation and chondrogenic capacity as compared to articular (HAC). To consider HNC a relevant alternative cell source for the repair of cartilage defects it is necessary test how these cells react when exposed environmental factors typical an injured joint. We thus aimed this study at investigating responses HAC exposure interleukin (IL)-1β low oxygen. For purpose harvested from same donors (N=5) were expanded in...
Standard cartilage tissue engineering approaches, for example, matrix-induced autologous chondrocyte implantation (MACI), consist of the cell-based constructs whose survival and further development first depend on degree graft maturity at time surgery (e.g., matrix production) and, subsequently, initial host reaction. Indeed, blood vessel ingrowth macrophage migration within implant may endanger stability immature constructs; so, control angiogenesis was proposed as an adjuvant cellular...
Abstract Engineered and devitalized hypertrophic cartilage (HC) has been proposed as bone substitute material, potentially combining the features of osteoinductivity, resistance to hypoxia, capacity attract blood vessels, customization potential for specific indications. However, in comparison with vital tissues, HC grafts have reduced efficiency formation longer remodeling times. We tested hypothesis that freshly harvested stromal vascular fraction (SVF) cells from human adipose...