A5030232429- Photodynamic Therapy Research Studies
- Nanoplatforms for cancer theranostics
- Hair Growth and Disorders
- Skin Protection and Aging
- Wound Healing and Treatments
- Luminescence Properties of Advanced Materials
- Neuroinflammation and Neurodegeneration Mechanisms
- Quantum Dots Synthesis And Properties
- Cancer and Skin Lesions
- Photoacoustic and Ultrasonic Imaging
- Genomics and Chromatin Dynamics
- Redox biology and oxidative stress
- Immune cells in cancer
- Luminescence and Fluorescent Materials
- Porphyrin and Phthalocyanine Chemistry
- Genomics, phytochemicals, and oxidative stress
- Wnt/β-catenin signaling in development and cancer
- Immune Cell Function and Interaction
- Epigenetics and DNA Methylation
- Bee Products Chemical Analysis
- Cancer-related gene regulation
- Gold and Silver Nanoparticles Synthesis and Applications
- Hedgehog Signaling Pathway Studies
- Planarian Biology and Electrostimulation
- Laser-Ablation Synthesis of Nanoparticles
Centro de Biología Molecular Severo Ochoa
2019-2025
Universidad Autónoma de Madrid
2016-2025
Consejo Superior de Investigaciones Científicas
2013-2024
Instituto Ramón y Cajal de Investigación Sanitaria
2014-2024
Research Institute Hospital 12 de Octubre
2019-2020
Durham University
2017-2019
Instituto de Investigaciones Biomédicas Sols-Morreale
2012-2018
Massachusetts General Hospital
2014-2017
Hospital Universitario Ramón y Cajal
2014-2016
Institució Catalana de Recerca i Estudis Avançats
2011
The effect of immunometabolism on age-associated diseases remains uncertain. In this work, we show that T cells with dysfunctional mitochondria owing to mitochondrial transcription factor A (TFAM) deficiency act as accelerators senescence. mice, these instigate multiple aging-related features, including metabolic, cognitive, physical, and cardiovascular alterations, which together result in premature death. cell metabolic failure induces the accumulation circulating cytokines, resembles...
The tremendous development of nanotechnology is bringing us closer to the dream clinical application nanoparticles in photothermal therapies tumors. This requires use specific that must be highly biocompatible, efficient light‐to‐heat converters and fluorescent markers. Temperature reading by heating during therapy appears paramount importance keep at a minimum collateral damage could arise from undesirable excessive heating. In this work, thermally controlled possible using Nd 3+ ion‐doped...
The future perspective of fluorescence imaging for real in vivo application are based on novel efficient nanoparticles which is able to emit the second biological window (1000–1400 nm). In this work, potential Nd 3+ ‐doped LaF 3 (Nd :LaF ) reported bioimaging both first and windows their three main emission channels ions: 4 F 3/2 → I 9/2 , 11/2 13/2 that lead emissions at around 910, 1050, 1330 nm, respectively. By systematically comparing relative intensities, penetration depths subtissue...
Nowadays, one of the most exciting applications nanotechnology in biomedicine is development localized, noninvasive therapies for diverse diseases, such as cancer. Among them, nanoparticle‐based photothermal therapy (PTT), which destroys malignant cells by delivering heat upon optical excitation nanoprobes injected into a living specimen, emerging with great potential. Two main milestones that must be reached PTT to become viable clinical treatment are deep penetration triggering and...
Quantum dot based-thermometry, in combination with double beam confocal microscopy and infrared thermal imaging, has been used to investigate the heating efficiency of multi-walled carbon nanotubes (MWCNTs) under optical excitation within first (808 nm) second (1090 biological windows as well spectral region separating them (980 nm). It found that for three wavelengths MWCNTs (10 nm diameter 1.5 μm length) is close 50%. Despite this "flat" efficiency, we have wavelength is, indeed, critical...
Over the past decade, near‐infrared (NIR)‐emitting nanoparticles have increasingly been investigated in biomedical research for use as fluorescent imaging probes. Here, high‐quality water‐dispersible core/shell/shell PbS/CdS/ZnS quantum dots (hereafter QDs) NIR probes fabricated through a rapid, cost‐effective microwave‐assisted cation exchange procedure are reported. These QDs proven to be water dispersible, stable, and expected nontoxic, resulting from growth of an outer ZnS shell...
Nanoparticle‐mediated photothermal therapy (NP‐PTT) constitutes a flexible, highly selective, cost effective, and accurate tool for cancer treatment alone or in combination with other therapies such as radiotherapy chemotherapy. The future application of NP‐PTT real life mainly depends on the design synthesis novel multifunctional nanoparticles that could overcome current limitations limited penetration depth absence control. In this work, ultrasmall (≈2.4 nm) NdVO 4 stoichiometric (100%...
Mammalian DNA methyltransferase 1 (DNMT1) is essential for maintaining methylation patterns after cell division. Disruption of DNMT1 catalytic activity results in whole genome cytosine demethylation CpG dinucleotides, promoting severe dysfunctions somatic cells and during embryonic development. While these observations indicate that DNMT1-dependent required proper function, the possibility has a role independent its matter controversy. Here, we provide evidence can support functions do not...
Abstract Photodynamic therapy (PDT) employing methyl δ‐aminolevulinic acid (Me‐ALA), as a precursor of the photosensitizer protoporphyrin IX (PpIX), is used for treatment non melanoma cutaneous cancer (NMCC). However, one problems PDT apparition resistant cell populations. The aim this study was to isolate and characterize squamous carcinoma cells SCC‐13 with Me‐ALA. parental population submitted successive cycles Me‐ALA‐PDT 10 populations were finally obtained. In there analyzed morphology...
Photodynamic therapy (PDT) is a treatment modality for different forms of cancer based on the combination light, molecular oxygen, and photosensitizer (PS) compound. When activated by PS generates reactive oxygen species leading to tumor destruction. Phthalocyanines are compounds that have already shown be efficient PSs PDT. Several examples carbohydrate substituted phthalocyanines been reported, assuming presence moieties could improve their selectivity. This work describes photoeffects...
The hair follicle is a biological oscillator that alternates growth, regression, and rest phases driven by the sequential activation of proliferation/differentiation programs resident stem cell populations. niches subsequent entry into growing phase mainly regulated Wnt/β-catenin signalling, while regression resting are Tgf-β/Bmp/Smad activity. A major question still unresolved nature molecular switch dictates coordinated transition between both signalling pathways. Here we have focused on...
Recent studies using single-cell RNA sequencing technology have uncovered several subpopulations of CD4+ T cells that accumulate with aging. These age-associated are emerging as relevant players in the onset inflammaging and tissue senescence. Here, based on information provided by data, we present a flow cytometry panel allows identification cell subsets systematic larger analysis mice. We use this to evaluate at level mitochondrial senescence marks different subpopulations. Our identifies...
Regenerative properties of skin decrease with age, and thus, the search for substances that minimize cutaneous ageing has increased in last few years. The secretion mollusc Cryptomphalus Aspersa (SCA) is a natural product bears regenerative when applied topically. purpose this work to study vitro effects SCA on cell proliferation migration, as well cell-cell (E-cadherin β-catenin) cell-substrate (vinculin β1-integrin) adhesion proteins expression, using human keratinocyte line (HaCaT cells)...
As an important component of tumor microenvironment, cancer-associated fibroblasts (CAFs) have lately gained prominence owing to their crucial role in the resistance therapies. Photodynamic therapy (PDT) stands out as a successful therapeutic strategy treat cutaneous squamous cell carcinoma. In this study, we demonstrate that transforming growth factor β1 (TGFβ1) cytokine secreted by CAFs isolated from patients with SCC can drive PDT epithelial cells. To end, obtained situ cSCC were firstly...
Achieving bright, reliable, robust, and stable probes for in vivo imaging is becoming extremely urgent the cancer research community. To date very few works have reported on elucidating varied chemically complex biological milieu. The authors report detailed investigations of synthesis near‐infrared, water dispersive, strongly luminescent, highly PbS/CdS/ZnS core/shell/shell quantum dots (QDs). These QDs are stable, they could keep their initial morphology, dispersion status,...