A5004669116- Immune Cell Function and Interaction
- Acute Myeloid Leukemia Research
- Nuclear Structure and Function
- Immunotherapy and Immune Responses
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Immune cells in cancer
- Viral Infections and Outbreaks Research
- T-cell and B-cell Immunology
- RNA Interference and Gene Delivery
- Eosinophilic Esophagitis
- interferon and immune responses
- Eosinophilic Disorders and Syndromes
- RNA Research and Splicing
- Nitric Oxide and Endothelin Effects
- Dietary Effects on Health
- Cancer Genomics and Diagnostics
- Immune Response and Inflammation
- Epigenetics and DNA Methylation
- Rabies epidemiology and control
- Cancer, Stress, Anesthesia, and Immune Response
- Venomous Animal Envenomation and Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Platelet Disorders and Treatments
- Atherosclerosis and Cardiovascular Diseases
- IL-33, ST2, and ILC Pathways
Instituto de Salud Carlos III
2025
Spanish National Centre for Cardiovascular Research
2013-2024
Human aging is frequently accompanied by the acquisition of somatic mutations in hematopoietic system that induce clonal hematopoiesis, leading to development a mutant clone progenitors and leukocytes. This somatic-mutation-driven hematopoiesis has been associated with an increased incidence cardiovascular disease type 2 diabetes, but whether this epidemiological association reflects direct, causal contribution immune cells age-related metabolic abnormalities remains unexplored. Here, we...
Somatic mutations in the TET2 gene that lead to clonal haematopoiesis (CH) are associated with accelerated atherosclerosis development mice and a higher risk of atherosclerotic disease humans. Mechanistically, these observations have been linked exacerbated vascular inflammation. This study aimed evaluate whether colchicine, widely available inexpensive anti-inflammatory drug, prevents TET2-mutant CH.
Antigen presentation by dendritic cells (DCs) stimulates naive CD4+ T cells, triggering cell activation and the adaptive arm of immune response. Newly synthesized major histocompatibility complex class II (MHC-II) molecules accumulate at MHC-II-enriched endosomal compartments are transported to plasma membrane DCs after binding antigenic peptides enable antigen presentation. In DCs, MHC-II included in tetraspanin-enriched microdomains (TEMs). However, role tetraspanin CD9 these processes...
Differentiation of naive CD4+ T-cells into functionally distinct T helper (Th) subsets is critical to immunity against pathogen infection. Little known about the role signals emanating from nuclear envelope for T-cell differentiation. The protein lamin A/C induced in upon antigen recognition and acts as a link between nucleus plasma membrane during activation. Here we demonstrate that absence reduces Th1 differentiation without affecting Th2 vitro vivo. Moreover, Rag1 -/- mice reconstituted...
Recent studies using single-cell RNA sequencing technology have uncovered several subpopulations of CD4+ T cells that accumulate with aging. These age-associated are emerging as relevant players in the onset inflammaging and tissue senescence. Here, based on information provided by data, we present a flow cytometry panel allows identification cell subsets systematic larger analysis mice. We use this to evaluate at level mitochondrial senescence marks different subpopulations. Our identifies...
Group 3 innate lymphoid cells (ILC3s) are vital for defending tissue barriers from invading pathogens. Hypoxia influences the production of intestinal ILC3-derived cytokines by activating HIF. Yet, mechanisms governing HIF-1α in ILC3s and other RORγt+ during vivo infections poorly understood. In our study, transgenic mice with specific Hif-1a gene inactivation (RAG1KO HIF-1α▵Rorc) exhibit more severe colitis following Citrobacter rodentium infection, primarily due to inability upregulate...
Abstract The mechanisms by which lamin A/C in CD4 + T‐cells control intestinal homeostasis and can cause inflammatory bowel disease (IBD) are unknown. Here, we explore a mouse model of IBD. Adoptive transfer to Rag1 −/− mice Lmna T‐cells, have enhanced regulatory (Treg) differentiation function, induced less severe IBD than wild‐type T‐cells. Lamin deficiency transcription the Treg master regulator FOXP3, thus promoting differentiation, reduced Th1 polarization, due epigenetic changes T‐bet....
Abstract Background Somatic mutations in the TET2 gene that lead to clonal hematopoiesis are associated with accelerated atherosclerosis development mice and a higher risk of atherosclerotic cardiovascular disease events humans. Mechanistically, these observations have been linked exacerbated vascular inflammation. Purpose Here, we sought evaluate whether colchicine, widely available inexpensive anti-inflammatory drug, prevents TET2-mutant hematopoiesis. Methods In mice, mimicked using...
Abstract Neutrophils are the most abundant leukocytes in blood, with numbers further increasing age. Despite their essential role as a primary line of defense, neutrophils can contribute to tissue damage and age-related diseases 1 high neutrophil-to-lymphocyte ratio predicts all causes mortality elderly 2–5 . However, precise mechanisms driving enhanced neutrophil generation during ageing remain poorly understood. Here, we show that subset CD4 + T cells cytotoxic phenotype (CD4 CTLs)...
Abstract Background Somatic mutations in blood indicative of clonal hematopoiesis indeterminate potential (CHIP), particularly DNMT3A , TET2 and JAK2 are associated with an increased risk hematologic malignancy, coronary artery disease, all-cause mortality. However, whether CHIP is peripheral disease (PAD) remains unknown. In addition, chemotherapy frequently causes DNA Damage Repair (DDR) genes TP53 PPM1D caused by somatic DDR results atherosclerosis unclear. We sought to test CHIP, genes,...
Quantification of naïve CD4 T cell activation, proliferation, and differentiation to helper 1 (Th1) cells is a useful way assess the role played by in an immune response. This protocol describes vitro bone marrow (BM) progenitors obtain granulocyte macrophage colony-stimulating factor (GM-CSF) derived-dendritic (DCs). The also adoptive transfer ovalbumin peptide (OVAp)-loaded GM-CSF-derived DCs from OTII transgenic mice order analyze vivo Th1 transferred cells. circumvents limitation purely...
Background: We have shown that pre-reperfusion metoprolol reduces infarct size in humans (METOCARD-CNIC trial). Preclinical data suggest this strategy myocardial neutrophil infiltration. Here we evaluated the potential involvement of circulating cells cardioprotection afforded by metoprolol.
Abstract Dendritic cells (DCs) play a crucial role in orchestrating immune responses, particularly promoting IFNγ-producing-CD8 cytotoxic T lymphocytes (CTLs) and IFNγ-producing -CD4 helper 1 (Th1) cells, which are essential for defending against viral infections. Additionally, the nuclear envelope protein lamin A/C has been implicated cell immunity. Nevertheless, intricate interplay between innate adaptive immunity response to infections, of DC functions within this context, remains poorly...