A5034193196- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Animal Nutrition and Physiology
- Parasitic Infections and Diagnostics
- Bacteriophages and microbial interactions
- Antimicrobial Resistance in Staphylococcus
- Mycobacterium research and diagnosis
- Urinary Tract Infections Management
- Escherichia coli research studies
- Tuberculosis Research and Epidemiology
- Amoebic Infections and Treatments
- Veterinary medicine and infectious diseases
- Listeria monocytogenes in Food Safety
- Genomics and Phylogenetic Studies
- Bacterial biofilms and quorum sensing
- Leptospirosis research and findings
- Aquaculture disease management and microbiota
- Pharmaceutical and Antibiotic Environmental Impacts
- Infective Endocarditis Diagnosis and Management
- Essential Oils and Antimicrobial Activity
- Parvovirus B19 Infection Studies
- Streptococcal Infections and Treatments
- Clostridium difficile and Clostridium perfringens research
Munster Technological University
2015-2024
Cork University Hospital
2002-2013
SUMMARY From January 2009 to May 2010, 436 faecal samples from patients with diarrhoeal illness in Southern Ireland were identified as Campylobacter genus-positive by an automated multiplex PCR; however, 204 (46·8%) of these culture-negative for campylobacters. A combination Campylobacter- specific uniplex PCR and 16S rRNA sequencing confirmed the presence DNA 191 (93·6%) samples. Species-specific C. jejuni (50·7%) ureolyticus (41%) coli (5·7%) most prevalent species while fetus ,...
The EntericBio system uses a multiplex PCR assay for the simultaneous detection of Campylobacter spp., Salmonella enterica, Shigella and Escherichia coli O157 from feces. It combines overnight broth enrichment with amplification by hybridization. An evaluation this was conducted comparing results obtained those routine culture, supplemented alternative methods. In study 773 samples, culture yielded 94.6 92.4% negative results, respectively. Forty-two samples had positive all these were...
Weissella is a genus of lactic acid bacteria (LAB) consisting species formerly included in the Leuconostoc paramesenteroides group. Similar to other LAB, they are commonly found fermented foods but have also been isolated from environmental and human samples. Currently there 20 recognized species. Herein, three cibaria genomes were sequenced using Illumia Mi-Seq Roche 454 technologies. Annotation was performed Prokka JGI IMG pipelines. A thorough analysis genomics W. strains performed,...
Infectious mononucleosis (IM) is an infectious disease that presents clinically in only a small percentage of individuals despite almost universal infection with the causative agent. Here, we review latest concepts clinical presentation, epidemiology, and host response this disease.
Infections with Campylobacter spp. pose a significant health burden worldwide. The significance of jejuni/Campylobacter coli infection is well appreciated but the contribution non-C. jejuni/C. to human gastroenteritis largely unknown. In this study, we employed two-tiered molecular study on 7194 patient faecal samples received by Microbiology Department in Cork University Hospital during 2009. first step, using EntericBio® (Serosep), multiplex PCR system, detected genus level. second...
A total of 7194 faecal samples collected over a 1-year period from patients presenting with diarrhoea were screened for Campylobacter spp. using EntericBio(®) , multiplex-PCR system. Of 349 Campylobacter-positive samples, 23.8% shown to be ureolyticus, combination 16S rRNA gene analysis and highly specific primers targeting the HSP60 this organism. This is, best our knowledge, first report C. ureolyticus in faeces gastroenteritis may suggest role organism as an emerging enteric pathogen.
Diagnostic molecular biology is arguably the fastest growing area in current laboratory-based medicine. Growth of so called 'omics' technologies has, over last decade, led to a gradual migration away from 'one test, one pathogen' paradigm, toward multiplex approaches infectious disease diagnosis, which have significant improvements clinical diagnostics and ultimately improved patient care.
In this study, we evaluated the use of EntericBio real-time Gastro Panel I (Serosep, Limerick, Ireland) for routine in a clinical microbiology laboratory simultaneous detection Campylobacter jejuni, coli, and lari, Shiga toxin-producing Escherichia coli (STEC), Salmonella spp., Shigella spp. feces. This system differs from its predecessor (the II system, Serosep) that it allows pathogens directly feces, without pre-enrichment. It also specifically detects lari rather than all species, as is...
An investigation of the prevalence Campylobacter ureolyticus in a variety animals led to identification strain CIT 045(T), faeces captive lion-tailed macaques (Macaca silenus). Originally, believed be based on colony morphology and positive urease test, analysis 16S rRNA hsp60 gene sequences this isolate revealed that differs significantly from other species genus described date. Species-specific primers for genes were designed used identify two additional strains isolated samples macaques....
Abstract The COVID-19 pandemic has resulted in increased need for diagnostic testing using reverse transcriptase real-time PCR (RT-PCR). An exponential increase demand a shortage of numerous reagents particular those associated with the lysis buffer required to extract viral RNA. Herein, we describe rapid collective effort by hospital laboratory scientists, academic researchers and biopharma industry generate validated buffer. We have formulated 4M Guanidinium thiocyanate (GITC)/ Triton...
Antibiotic resistance is increasing to an extent where efficacy not guaranteed when treating infection. Biofilm formation has been shown complicate treatment, whereby the of biofilm associated with higher minimum inhibitory concentration values antibiotic. The objective current paper was determine whether variable among uropathogenic Escherichia coli isolates and recurrent urinary tract infection (UTI), it can be predicted by phenotypic appearance on culture medium A total 62 E. that were...
With antimicrobial resistance rising globally, the exploration of alternative sources candidate molecules is critical to safeguard effective chemotherapeutics worldwide. Plant natural products are accessible, structurally diverse compounds with potential. The pharmacological applications plants in medicine can be guided by attestation traditional use, as demonstrated this study. In Irish ethnomedical literature, Inula helenium L. (elecampane) often indicated for respiratory and dermal...
Measurements were made of the susceptibility to six commonly prescribed antibiotics, including erythromycin, tetracycline and ciprofloxacin, 130 isolates Campylobacter jejuni 15 coli cultured from human poultry sources during 2000. The results compared with a collection strains isolated between 1996 1998. levels resistance erythromycin remained low, 2 per cent 4.4 for isolates, respectively. Resistance had increased 31 24.4 13.9 18.8 However, ciprofloxacin 2000 30 cent, whereas 1998 there...
The aim was to exploit whole genome sequencing (WGS) assess genomic diversity, identify virulence genes and deduce the proportion of Campylobacter colonized broilers that directly contaminate their carcasses.Campylobacter jejuni isolates (107) from caeca carcass neck skin samples (50 pairs same batch plus 7 individual caeca) sampled at three poultry slaughterhouses over a one-year period were selected for (MiSeq; Illumina). FastQ files submitted BioNumerics analysis using wgMLST scheme...
Resistance to antimicrobial agents used treat severe Campylobacter spp. gastroenteritis is increasing worldwide. We assessed the resistance patterns of isolates human and animal origin. More than half (n = 32) were resistant sulphonamide, a feature known be associated with presence integrons. Analysis these integrons will further our understanding epidemiology.