A5060506633- Salmonella and Campylobacter epidemiology
- Animal Nutrition and Physiology
- Viral gastroenteritis research and epidemiology
- Leptospirosis research and findings
- Listeria monocytogenes in Food Safety
- Pharmaceutical and Antibiotic Environmental Impacts
- Veterinary medicine and infectious diseases
- Antibiotic Resistance in Bacteria
- Food Safety and Hygiene
- Animal Disease Management and Epidemiology
- Aquaculture disease management and microbiota
- Essential Oils and Antimicrobial Activity
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Probiotics and Fermented Foods
- Biopolymer Synthesis and Applications
- Microscopic Colitis
- Agriculture, Soil, Plant Science
- Livestock and Poultry Management
- Ruminant Nutrition and Digestive Physiology
- Turfgrass Adaptation and Management
University College Dublin
2016-2022
Teagasc - The Irish Agriculture and Food Development Authority
2015-2019
Irish Horseracing Regulatory Board (Ireland)
2019
University of Georgia
2002
Salmonella is a global threat for public health, and pork one of the main sources human salmonellosis. However, complex epidemiology infection limits current control strategies aimed at reducing prevalence this in pigs. The present study analyzes first time impact gut microbiota pigs its shedding pattern naturally infected growing Microbiome (16S rRNA amplicon) analysis reveals that maturation microbiome could be key consideration with respect to limiting Indeed, seronegative animals had...
The aim was to exploit whole genome sequencing (WGS) assess genomic diversity, identify virulence genes and deduce the proportion of Campylobacter colonized broilers that directly contaminate their carcasses.Campylobacter jejuni isolates (107) from caeca carcass neck skin samples (50 pairs same batch plus 7 individual caeca) sampled at three poultry slaughterhouses over a one-year period were selected for (MiSeq; Illumina). FastQ files submitted BioNumerics analysis using wgMLST scheme...
Campylobacteriosis is the leading cause of human bacterial gastroenteritis, very often associated with poultry consumption. Thermophilic Campylobacter (Campylobacter jejuni and coli) isolates (n = 158) recovered from broiler neck skin caecal contents in Ireland over a one-year period, resistant to at least one three clinically relevant antimicrobial classes, were screened for resistance determinants. All ciprofloxacin-resistant 99) harboured C257T nucleotide mutation (conferring Thr-86-Ile...
Salmonella carriage in pigs is a significant food safety issue. This study describes new protocol of infection based on exposure to an artificially contaminated environment that closely mimics natural the organism. The aim was develop and evaluate effectiveness this protocol, which could then be used as tool investigation control measures. In addition, shedding pattern growth performance were examined. Trial (n = 10) placed pen had been previously by housing two experimentally challenged...
This study examined the impact of key processing stages and flock variables on prevalence Campylobacter broiler carcasses. Overall, was 62% in caeca, 68%, 65% neck skin samples collected after evisceration, final wash carcass chilling, respectively. were found 32% 52%, 40% respectively from first thin batches. Final batches more frequently contaminated with prevalences 83% 80% evisceration both chilling (p < 0.05). Thinning status had a significant effect counts significantly higher observed...
Summary This study aimed to provide new insights into the epidemiology of Salmonella in pig production, focusing on potential shedding patterns breeding pigs throughout a full production cycle and risk transmission infection from sow her offspring. A longitudinal was conducted five farrow‐to‐finish commercial farms. In each herd, faeces monitored breeders through service, gestation lactation. Swabs farrowing room floor pools piglets were collected two occasions during Environmental pen swabs...
Among the zoonotic pathogens affecting pigs, Salmonella stands out due to high number of human cases linked pork consumption. In last two decades many countries have put considerable effort into control infection by surveillance and strategies on farm. Despite this effort, herds still a prevalence they require guidance address problem. The present study, using serological data finishing pigs from Irish National pig Control Programme, aimed highlight factors associated with increased risk or...
1. Campylobacteriosis is the leading cause of human bacterial gastroenteritis. Broilers are considered most important source Campylobacter infection. In 2008 European baseline survey Ireland had a 98% prevalence campylobacter-contaminated broiler carcases. 2. Randomly-selected isolates (296 C. jejuni, 54 coli) recovered in 2017 and 2018, from Irish neck skin caeca were tested for their resistance to tetracycline, erythromycin, gentamicin, ciprofloxacin, nalidixic acid streptomycin. 3....
This research tested the anti-Campylobacter properties of organic acids (OA), medium chain fatty (MCFA) and essential oils (EO) in vitro commenced vivo suitability testing focused on broiler performance.Nine active compounds were at different concentrations times against Campylobacter jejuni sterile distilled water, Mueller Hinton broth grower feed digestate (GFD). Sodium caprate (1.5%, v/v), thymol (0.25% 2.5%, carvacrol (1.25%, v/v) potassium sorbate each achieved C. reductions ≥4.5 log10...
16S rRNA amplicon sequencing was used to investigate changes in the broiler gastrointestinal tract (GIT) microbiota throughout rearing period and combination with antibiotic treatment. Thirty birds (from a commercial flock) were removed at multiple points on days 13, 27, 33, euthanised, their GIT aseptically divided into upper (the crop, proventriculus, gizzard), middle duodenum, jejunum, ileum) lower large intestine, caeca, cloaca) sections. In separate flock, same farm similar husbandry...
The true prevalence of tet(A), which codes for a tetracycline efflux pump, in thermophilic Camplyobacter spp. requires clarification after reports emerged Iran (2014) and Kenya (2016) the novel detection tet(A) Campylobacter. During our investigation antibiotic resistance mechanisms sample Irish Campylobacter broiler isolates, it was determined that 100% tetracycline-resistant isolates (n = 119) harboured tet(O). Accessory tetracycline-resistance were considered as minimum inhibitory...
Campylobacter spp. is the leading cause of bacterial gastroenteritis worldwide and poultry are primary reservoir. The aim this study was to investigate survival and/or growth C. jejuni NCTC 11168 in broiler digestate prepared from commercial starter, grower finisher feed formulations. Bolton broth digestates were prepared, inoculated with (approximately 3 log10 CFU per ml) incubated under microaerobic conditions at 42°C for 24 hours. Samples taken t = 0 (immediately after inoculation) every...
This study aimed to investigate the effectiveness of commercially available sodium butyrate control shedding Salmonella on two Irish pig farms with a history high seroprevalence. On both farms, pens (12-17 pigs/pen) were randomly assigned (finisher feed without additives) or an acid treatment (the same supplemented 0.03% butyrate) for 24-26 days prior slaughter. Farm A, was reduced in group compared at end period (30% vs. 57% probability detecting faeces, respectively; p<0.05). However no...
This study investigates the effect of stocking density and population dynamics on broiler growth rates productivity, while further validating ability biosecurity cubes (BC) to protect birds from Campylobacter. In our methodology, six BC were constructed in a commercial house containing approximately 28,500 birds. During three trials, stocked at densities 12, 14, 16, 18, 20 22 birds/m2, with main flock (20 birds/m2) considered control. Periodically, 10 per weighed examined. The Campylobacter...
Campylobacter is the most common foodborne pathogen in developed countries and cases are associated with poultry. This study investigated effect of three anti-Campylobacter water additives on broiler growth caecal microbiota at harvest using 16S rRNA amplicon sequencing. Mixtures organic acids (OA) essential oils (EO) were administered to broilers for entirety production cycle (35 d) medium-chain fatty (MCFA) 5 d immediately before harvest, under commercial conditions. Bird weight gain was...