A terbium complex with long luminescence lifetime for the selective time-gated detection of potassium is presented. The demonstrates high sensitivity a 25-fold increase in intensity between 0 and 15 mM potassium. selectivity over other physiological cations, especially sodium (93 fold), likely due to favorable cation−π interaction phenol moiety ion.

The magnetic properties of iron oxide nanoparticles govern their relaxivities and efficacy as contrast agents for MRI. These are in turn determined by composition, size morphology. Herein we present a systematic study the effect particle shape magnetite nanocrystals synthesized thermal decompositions salts on both magnetism longitudinal transverse relaxivities, r1 r2, respectively. Faceted demonstrate superior than spherical similar size. For faceted nanoparticles, but not ones, r2 further...

A molecular probe for the luminescent detection of adenosine nucleotides is presented. The probe, Tb-DOTAm-Phen, readily distinguishes among three in buffered aqueous conditions at neutral pH, a requirement direct monitoring enzymatic reactions converting triphosphate (ATP) to diphosphate or monophosphate. most efficient under millimolar concentrations ATP which are relevant intracellular conditions. Moreover, long luminescence lifetime enables time-gating experiments.

The molecular weight, purity, and functionalization of polyethylene glycols are often characterized by 1H NMR spectroscopy. Oft-forgotten, the typical pulse sequence is not 13C decoupled. Hence, for large polymers, coupled peaks arising from repeating units have integrations comparable to that terminal groups. Ignoring this coupling leads erroneous assignments. Once correctly assigned, these can be used determine both weight polymer efficacy conjugation a moiety more accurately than uncoupled unit.

Abstract Hydrophobic magnetite nanoparticles synthesized from thermal decomposition of iron salts must be rendered hydrophilic for their application as MRI contrast agents. This process requires refunctionalizing the surface with a organic coating such polyethylene glycol. Two parameters were found to influence magnetic behavior and relaxivity resulting oxide nanoparticles: functionality anchoring group protocol followed functionalization. Nanoparticles coated PEGs via catecholate‐type...

One essential requirement for more sensitive gadolinium-based MRI contrast agents is to slow the molecular tumbling of gadolinium(III) ion, which increases gadolinium's relaxivity (i.e., its ability speed up NMR relaxation nearby water molecules). route this through conjugation high-molecular-weight polymers such as dendrimers. In work, amine-functionalized TREN-bis(1,2-HOPO)-TAM-ethylamine and TREN-bis(1-Me-3,2-HOPO)-TAM-ethylamine ligands have been synthesized attached biocompatible 40 kDa...

Impairment in glomerular endothelial function likely plays a major role the development of albuminuria and CKD progression. Glomerular dysfunction may reflect systemic microvascular dysfunction, accounting part for greater cardiovascular risk patients with albuminuria. Prior studies vascular have focused on conduit artery or those ESRD, not examined We conducted cross-sectional study among older hypertensive male veterans stage 1–4 CKD, controls without CKD. Microvascular was quantified by...

Self-assembly of metallointercalators into DNA nanocages is a rapid and facile approach to synthesize discrete bioinorganic host/guest structures with high load metal complexes. Turberfield's tetrahedron can accommodate one intercalator for every two base pairs, which corresponds 48 per tetrahedron. The affinity the metallointercalator function both structure intercalating ligand overall charge complex, trend in [Ru(bpy)

We report the 1.1-Å resolution crystal structure of a bulky rhodium complex bound to two different DNA sites, mismatched and matched in oligonucleotide 5′-(dCGG A AATT C CCG) 2 -3′. At AC mismatch site, reveals ligand insertion from minor groove with ejection both bases elucidates how destabilized mispairs may be recognized. This unique binding mode contrasts major intercalation, observed at where doubling base pair rise accommodates stacking intercalator. Mass spectral analysis...

Two crystal structures of Δ-Rh(bpy)2(chrysi)3+ (chrysi is 5,6-chrysenequinone diimine) bound to the oligonucleotide duplex 5′-CGGAAATTACCG-3′ containing two adenosine-adenosine mismatches (italics) through metalloinsertion were determined. Diffraction quality crystals with different space groups (P3221 and P43212) obtained under very similar crystallization conditions. In both structures, bulky rhodium complex inserts into mismatched sites from minor groove side, ejecting bases major groove....

The synthesis and characterization of core-shell Fe(3)O(4)@organic@Au nanoparticles displaying plasmonic behavior, high magnetism, relaxivity is presented. incorporation a thin organic layer between the two metals crucial in maintaining saturation magnetisation superparamagnetic core.

Two molecular probes for the detection of nucleotides are presented. The positively charged [Tb-DOTAm-Phen]3+ responds only to purine and selectively detects GTP ATP over GDP/GMP ADP/AMP, respectively. neutral Eu-DOTA-Phen complex, on other hand, is selective purines pyrimidines but does not distinguish between tri-, di- monophosphate nucleotides. Both possess long luminescence lifetimes that readily enable time-gated experiments complete removal any autofluorescence background. decrease...

A terbium probe for the selective time-gated detection of hydroxyl radical is presented. This demonstrates high sensitivity with an 11.0-fold increase in luminescence intensity hour-long exposure to concentration femtomolar range, and selectivity HO˙ over other reactive oxygen species.

A series of fluorinated macrocyclic complexes, M-DOTAm-F12, where M is LaIII, EuIII, GdIII, TbIII, DyIII, HoIII, ErIII, TmIII, YbIII, and FeII, was synthesized, their potential as fluorine magnetic resonance imaging (MRI) contrast agents evaluated. The high water solubility these complexes the presence a single NMR signal, two necessary parameters for in vivo MRI, are substantial advantages over currently used organic polyfluorocarbons other reported paramagnetic 19F probes. Importantly,...

Magnetic resonance imaging (MRI) has emerged over the years as one of preferred modalities for medical diagnostic and biomedical research. It advantage other such positron emission tomography X-ray affording high resolution three-dimensional images body without using harmful radiation. The use contrast agents further expanded this technique by increasing between regions where they accumulate background tissues. As MRI most often measures relaxation rate water throughout body, function...

Electrodeposited Fe and Fe-Au nanowires were studied for potential as MRI contrast agents, especially T2-weighted imaging. Transverse relaxivities up to r2 = 77.1 mM Fe-1 s-1 (at 1.5 T) achieved when coated with thiol carboxylic acid functionalized poly(ethyleneglycol). T2-Weighted images (9 verified successful contrast.

Chelation approaches that are compatible with a multitude of isotopes an important area development. Here, we introduce the design, synthesis, and evaluation 2,3-dihydroxyterephthalate/catechol chelator conjugates positron emission tomography (PET) 68Ga3+ 45Ti4+, targeting prostate-specific membrane antigen (PSMA). The made in multistep organic synthesis incorporating 2,3-dihydroxyterephthalate, linked to amino hexanoic acid-extended, urea-dipeptides EuE or KuE (substrates PSMA active site)....

Hydroxypyridinone (HOPO)-based Gd(III) complexes have previously been shown to exhibit high relaxivity, especially at the magnetic fields that are clinically relevant for present and future clinical use. This is due more than one coordinated water molecule exchanging rapidly with bulk solvent. These complexes, however, poor solubility. Heteropodal which include a terephthalamide (TAM) moiety maintain relaxivity characteristics of HOPO family functionalized solubilizing substituents various...