A5074474641- Spectroscopy Techniques in Biomedical and Chemical Research
- Near-Field Optical Microscopy
- Phytochemicals and Antioxidant Activities
- Advanced Glycation End Products research
- Research on Leishmaniasis Studies
- Advanced biosensing and bioanalysis techniques
- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced Biosensing Techniques and Applications
- Biochemical and biochemical processes
- Advanced X-ray and CT Imaging
- Biochemical effects in animals
- Advanced Fluorescence Microscopy Techniques
- Lipid Membrane Structure and Behavior
- Biosensors and Analytical Detection
- Insect Pest Control Strategies
- Plasmonic and Surface Plasmon Research
- Nanoparticles: synthesis and applications
- Thermography and Photoacoustic Techniques
- Quantum Dots Synthesis And Properties
- Bee Products Chemical Analysis
- Optical Imaging and Spectroscopy Techniques
- thermodynamics and calorimetric analyses
- Advanced Chemical Sensor Technologies
- Molecular Biology Techniques and Applications
- Antimicrobial Peptides and Activities
Institute of Cancer Research
2024
Physikalisch-Technische Bundesanstalt
2013-2023
Humboldt-Universität zu Berlin
2013
Federal Institute For Materials Research and Testing
2013
We describe the application of scattering-type near-field optical microscopy to characterize various semiconducting materials using electron storage ring Metrology Light Source (MLS) as a broadband synchrotron radiation source.For verifying high-resolution imaging and nano-FTIR spectroscopy we performed scans across nanoscale Si-based surface structures.The obtained results demonstrate that spatial resolution below 40 nm can be achieved, despite use source with an extremely broad emission...
We correlate the localization of silver nanoparticles inside cells with respect to cellular architecture molecular information in vicinity particle surface by combining nanoscale 3D cryo-soft X-ray tomography (cryo-SXT) surface-enhanced Raman scattering (SERS). The interaction nanoparticle small molecules and biopolymers was monitored SERS vitro over time living cells. spectra indicate a stable, time-independent composition nanoparticles, despite changing environment endosomal structure....
A main challenge in understanding the structure of a cell membrane and its interactions with drugs is ability to chemically study different molecular species on nanoscale. We have achieved this for model system consisting mixed monolayers (MLs) biologically relevant phospholipid 1,2-distearoyl-sn-glycero-phosphatidylcholine antibiotic surfactin. By employing nano-infrared (IR) microscopy spectroscopy combination atomic force imaging, it was possible identify detect domain formation two...
Infrared scattering scanning near-field optical microscopy (IR s-SNOM) provides for spectroscopic imaging with nanometer spatial resolution, yet full spatio-spectral is constrained by long measurement times.Here, we demonstrate the application of compressed sensing algorithms to achieve hyperspectral FTIR-based nano-imaging at an order magnitude faster speed same spectral content compared conventional approaches.At example spectroscopy a single vibrational resonance, discuss relationship...
Abstract The chemical composition of melanoidins formed from glucose (Glc) and alanine (Ala) in different molar ratios was investigated using UV/Vis, FTIR, EPR spectroscopy elemental analysis (EA). Melanoidin samples were prepared at varying Glc Ala ranging 10:1 to 1:10 (Glc:Ala). Reaction systems containing a higher ratio show melanoidin yields UV/Vis absorbance. This indicates that an excess facilitates the formation larger π -electron catalyzes formation. showed more radicals enriched...
Leishmania species are protozoan parasites and the causative agents of leishmaniasis, a vector borne disease that imposes large health burden on individuals living mainly in tropical subtropical regions. Different responsible for distinct clinical patterns, such as cutaneous, mucocutaneous, visceral with latter being potentially fatal if left untreated. For this reason, it is important to perform correct identification differentiation. Fourier transform infrared spectroscopy (FTIR) an...
Abstract Synchrotron radiation‐based Fourier transform infrared spectroscopy enables access to vibrational information from mid over far even terahertz domains. This may prove critical for the elucidation of fundamental bio‐molecular phenomena including folding‐mediated innate host defence mechanisms. Antimicrobial peptides (AMPs) represent one such phenomena. These are major effector molecules immune system, which favour attack on microbial membranes. AMPs recognise and bind membranes...
Abstract Infrared (IR) hyperspectral imaging is a powerful approach in the field of materials and life sciences. However, for extension to modern sub-diffraction nanoimaging it still remains highly inefficient technique, as acquires data via inherent sequential schemes. Here, we introduce mathematical technique low-rank matrix reconstruction scheme atomic force microscopy-based infrared spectroscopy (AFM-IR), efficient IR nanoimaging. To demonstrate its application potential, chose...
Fourier transform infrared (FTIR) spectroscopy is a powerful technique in analytical chemistry. Typically, spatially distributed spectra of the substance interest are conducted simultaneously using FTIR spectrometers equipped with array detectors. Scanning-based methods such as near-field spectroscopy, on other hand, promising alternative providing higher spatial resolution. However, serial recording severely limits their application due to long acquisition times involved and resulting...
The need for technological progress in bio-diagnostic assays of high complexity requires both fundamental research and constructing efforts on nano-scaled assay recognition elements that can provide unique selectivity design-enhanced sensitivity features. Nanoparticle induced enhancement its application related to multiplexed capability Surface-Enhanced InfraRed Absorption (SEIRA) formats are well suitable these purposes. potential diverse fluorophore-antibody conjugates, being chemisorbed...
An improvement in the reliability and comparability of tissue characterization results is crucial for enabling further progress cancer detection assessment therapeutic effects. This can only be achieved by integrating quantitative methods into well-established qualitative routines. case study presents a hybrid metrological approach characterisation including vibrational Fourier Transform InfraRed (FTIR) spectroscopy traceable reference-free X-Ray Fluorescence analysis (XRF). Through...
Bio-diagnostic assays of high complexity rely on nanoscaled assay recognition elements that can provide unique selectivity and design-enhanced sensitivity features. High throughput performance requires the simultaneous detection various analytes combined with appropriate bioassay components. Nanoparticle induced enhancement, subsequent multiplexed capability Surface-Enhanced InfraRed Absorption (SEIRA) formats are fitting well these purposes. SEIRA constitutes an ideal platform to isolate...
In Fourier transform infrared (FTIR) spectral imaging spatially distributed spectra of 2D surfaces are registered simultaneously using FTIR spectrometers equipped with array detectors. Scanning methods such as near-field spectroscopy a promising alternative providing both, higher spatial resolution, and sensitivity. However, serial recording severely limits their application due to long acquisition times involved the resulting stability issues. this work we demonstrate different strategies...
Infrared hyperspectral imaging is a powerful approach in the field of materials and life sciences. However, extension to modern sub-diffraction nanoimaging still remains highly inefficient technique, as it acquires data via inherent sequential schemes. Here, we introduce mathematical technique low-rank matrix reconstruction scheme atomic force microscopy-based infrared spectroscopy (AFM-IR), for efficient nanoimaging. To demonstrate its application potential, chose trypanosomatid unicellular...