A5053893695- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Parasites and Host Interactions
- Insect Pest Control Strategies
- Zoonotic diseases and public health
- Leptospirosis research and findings
- Digital Imaging for Blood Diseases
- Phytochemistry and biological activities of Ficus species
- Botanical Studies and Applications
- History of Medicine and Tropical Health
- Viral Infections and Vectors
- Insect Resistance and Genetics
- Vector-Borne Animal Diseases
- Spectroscopy and Chemometric Analyses
- Vector-borne infectious diseases
- Nuts composition and effects
- Insect and Pesticide Research
Technical University of Applied Sciences Wildau
2014-2021
Leibniz Centre for Agricultural Landscape Research
2020-2021
Sechenov University
2020
Charité - Universitätsmedizin Berlin
2006-2014
Institute of Medical Microbiology and Hygiene
2006-2014
Institut für Hygiene und Umwelt
2009
Leishmaniasis is a geographically widespread severe disease, with an increasing incidence of two million cases per year and 350 people from 88 countries at risk. The causative agents are species Leishmania, protozoan flagellate. Visceral leishmaniasis, the most form lethal if untreated, caused by Leishmania donovani complex. These morphologically indistinguishable but have been identified molecular methods, predominantly multilocus enzyme electrophoresis. We conducted multifactorial genetic...
Leishmania infantum (syn. L. chagasi) is the causative agent of visceral leishmaniasis (VL) in New World (NW) with endemic regions extending from southern USA to northern Argentina. The two hypotheses about origin VL NW suggest (1) recent importation Old (OW), or (2) an indigenous and a distinct taxonomic rank for parasite. Multilocus microsatellite typing was applied survey 98 isolates different foci. profiles obtained were compared those 308 20 donovani strains OW countries previously...
BackgroundLeishmania infantum is the causative agent of visceral and cutaneous leishmaniasis in Mediterranean region, South America, China. MON-1 L. predominating zymodeme all endemic regions, both humans dogs, reservoir host. In order to answer important epidemiological questions it essential discriminate strains MON-1.Methodology/Principal FindingsWe have used a set 14 microsatellite markers analyse 141 mainly from Spain, Portugal, Greece which 107 were typed by MLEE as MON-1. The highly...
Background Parasites' evolution in response to parasite-targeted control strategies, such as vaccines and drugs, is known be influenced by their population genetic structure. The aim of this study was describe the structure Ethiopian strains Leishmania donovani derived from different areas endemic for visceral leishmaniasis (VL) a prerequisite design effective strategies against disease. Methodology/Principal Findings Sixty-three L. newly isolated VL cases two main foci, north Ethiopia (NE)...
Background New foci of human CL caused by strains the Leishmania donovani (L. donovani) complex have been recently described in Cyprus and Çukurova region Turkey infantum) situated 150 km north Cyprus. Cypriot were typed Multilocus Enzyme Electrophoresis (MLEE) using Montpellier (MON) system as L. zymodeme MON-37. However, multilocus microsatellite typing (MLMT) has shown that this is paraphyletic; composed distantly related genetic subgroups different geographical origin. Consequently...
Background/Objectives: Parasites of the subgenus Leishmania (Viannia) cause varying clinical symptoms ranging from cutaneous leishmaniases (CL) with single or few lesions, disseminated CL (DL) multiple lesions to disfiguring forms mucocutaneous leishmaniasis (MCL). In this population genetics study, 37 strains L. (V.) guyanensis, 63 braziliensis, four shawi, six lainsoni, seven naiffi, one each utingensis and lindenbergi, lainsoni/L. naiffi hybrid different endemic foci in Brazil were...
A multilocus microsatellite typing (MLMT) approach based on the analysis of 15 independent loci has been developed for discrimination strains belonging to different Viannia species. Thirteen were isolated de novo from microsatellite-enriched libraries both Leishmania braziliensis and L. guyanensis. Two previously identified markers, AC01 AC16, modified added our marker set. Markers designed contain simple dinucleotide repeats flanked by minimal possible number nucleotides in order allow...
We report paired strains of Leishmania parasites, one from the viscera and other skin lesions that were isolated three patients with visceral leishmaniasis disseminated cutaneous co-infected human immunodeficiency virus. The causative parasites characterized by polymerase chain reaction–restriction length polymorphism ribosomal DNA internal transcribed spacer 1 a panel multilocus microsatellite markers. demonstrated agent was donovani in all cases, irrespective phenotype disease. same showed...
Summary Detection of Leishmania parasites in a clinical sample is necessary to confirm suspected case leishmaniasis. We compared the sensitivity internal transcribed spacer 1‐PCR (ITS 1‐PCR) assay for parasite diagnosis with that microscopic detection samples from kala‐azar (KA) or post‐kala‐azar dermal leishmaniasis (PKDL) suspects Mymensingh. Of 39 specimens collected 35 KA and four PKDL suspects, 26 were positive by examination smears bone marrow skin exudates; 38 spotted on filter paper...
Our present study is the first attempt to characterize Leishmania parasites from foci in Uzbekistan and Tajikistan endemic for visceral leishmaniasis (VL). PCR-sequencing of ribosomal internal transcribed spacer 1 multilocus microsatellite typing (MLMT) were applied DNA extracted preparations Giemsa-stained bone marrow aspirates 13 cases VL. L. infantum was shown cause VL currently occurring this area. MLMT applying 14 markers, previously be polymorphic strains donovani complex, revealed...