A5076132020- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Parasites and Host Interactions
- Reproductive Biology and Fertility
- Vector-borne infectious diseases
- Insect Pest Control Strategies
- Viral Infections and Vectors
- Insect and Pesticide Research
- Insect symbiosis and bacterial influences
- Reproductive Physiology in Livestock
- Animal Genetics and Reproduction
- Evolution and Genetic Dynamics
- T-cell and Retrovirus Studies
- Renal and related cancers
- Pluripotent Stem Cells Research
- Ovarian function and disorders
- Chromosomal and Genetic Variations
- Parasite Biology and Host Interactions
- Zoonotic diseases and public health
- Plant Pathogens and Fungal Diseases
- Heat shock proteins research
- Genetic and phenotypic traits in livestock
- S100 Proteins and Annexins
- Fungal Infections and Studies
- Antifungal resistance and susceptibility
Fundação Oswaldo Cruz
2016-2025
Faculdades Oswaldo Cruz
2012
Universidade Federal Fluminense
2008-2011
In this study, PCR assays targeting different Leishmania heat-shock protein 70 gene (hsp70) regions, producing fragments ranging in size from 230-390 bp were developed and evaluated to determine their potential as a tool for the specific molecular diagnosis of cutaneous leishmaniasis (CL). A total strains analysed, including seven reference (RS) 63 previously typed strains. Analysis RS indicated region 234 hsp70 valid target that was highly sensitive detection species DNA with capacity...
Protozoan parasites of the genus Leishmania adapt to environmental change through chromosome and gene copy number variations. Only little is known about external or intrinsic factors that govern genomic adaptation. Here, by conducting longitudinal genome analyses 10 new clinical isolates, we uncovered important differences in among genetically highly related strains revealed gain loss copies as potential drivers long-term adaptation field. In contrast, rather than amplification was...
The Leishmania genus comprises up to 35 species, some with status still under discussion. multilocus sequence typing (MLST)--extensively used for bacteria--has been proposed pathogenic trypanosomatids. For Leishmania, however, a detailed analysis and revision on the taxonomy is required. We have partially sequenced four housekeeping genes--glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate (6PGD), mannose phosphate isomerase (MPI) isocitrate (ICD)--from 96 (Viannia) strains...
Leishmania infantum causes visceral leishmaniasis, a deadly vector-borne disease introduced to the Americas during colonial era. This non-native trypanosomatid parasite has since established widespread transmission cycles using alternative vectors, and human infection become significant concern public health, especially in Brazil. A multi-kilobase deletion was recently detected Brazilian L. genomes is suggested reduce susceptibility anti-leishmanial drug miltefosine. We show that...
Canine Visceral Leishmaniasis (CVL) shares many aspects with the human disease and dogs are considered main urban reservoir of L. infantum in zoonotic VL. Infected develop progressive a large clinical spectrum. A complex balance between parasite genetic/immunological background host decisive for infection evolution outcome. This study comprised 92 Leishmania infected mongrel various ages from Mato Grosso, Brazil. Spleen samples were collected determining load, humoral response, cytokine mRNA...
Background The present study investigates implications of a sub-chromosomal deletion in Leishmania infantum strains, the causative agent American Visceral Leishmaniasis (AVL). Primarily found New World leads to absence ecto-3’-nucleotidase/nuclease enzyme, impacting parasite virulence, pathogenicity, and drug susceptibility. factors favoring prevalence widespread geographic distribution these deleted mutant parasites (DEL) NW (NW) are discussed under generated data. Methods We conducted...
Background/Objectives: Parasites of the subgenus Leishmania (Viannia) cause varying clinical symptoms ranging from cutaneous leishmaniases (CL) with single or few lesions, disseminated CL (DL) multiple lesions to disfiguring forms mucocutaneous leishmaniasis (MCL). In this population genetics study, 37 strains L. (V.) guyanensis, 63 braziliensis, four shawi, six lainsoni, seven naiffi, one each utingensis and lindenbergi, lainsoni/L. naiffi hybrid different endemic foci in Brazil were...
SUMMARY Some Trypanosoma and Leishmania species are multi-host parasites whose distribution overlaps in several parts of the Brazilian Amazon basin. Despite being a common trait among wild mammals, mixed infections their consequences for host's health parasite transmission still poorly known phenomenon. Here we describe triple infection – cruzi , T. rangeli infantum bone marrow sample from an anteater Tamandua tetradactyla captured house backyard endemic Abaetetuba municipality was also...
The aim of this study was to evaluate the effect culture media and stage development in osmotic ability vitro-fertilized bovine embryos expression aquaporin 3 (Aqp3) Na/K ATPase isoform 1 (ATPAse1) genes (i) with different undergo rehydration (ii) following vitrification. In experiment 1, vitro fertilized presumptive zygotes were co-cultured SOFaac or modified CR2aa medium at blastocyst expanded stages day 7 post-insemination exposed NaCl hypertonic (900 mOsm) for 5 min 120 isotonic order...
Background Caviomorph rodents, some of the oldest Leishmania spp. hosts, are widely dispersed in Brazil. Despite both experimental and field studies having suggested that these rodents potential reservoirs parasites, not more than 88 specimens were analyzed few natural infection. Our hypothesis was caviomorph inserted transmission cycles different regions, so is currently recognized. Methodology We investigated infection spleen fragments 373 from 20 species collected five Brazilian biomes a...
Leishmaniasis is a worldwide neglected disease, encompassing asymptomatic infections and different clinical forms, such as American Tegumentary (ATL) which part of the complex diseases caused by protozoan parasites from Leishmania genus, transmitted sand fly vectors. As much effort still needed in treatment diagnosis. Currently, ATL diagnosis mainly made parasite detection microscopy. The sensitivity method varies, factors collection procedures interfere. Molecular approaches, specially...
As the distribution of Candida species and their susceptibility to antifungal agents have changed, a new means accurately rapidly identifying these is necessary for successful early resolution infection subsequent reduction morbidity mortality. The current work aimed evaluate ribosomal RNA gene sequencing identification medically relevant in comparison with standard phenotypic method. Eighteen reference strains (RSs), 69 phenotypically identified isolates 20 inconclusively were examined....
With the emergence of leishmaniasis in new regions around world, molecular epidemiological methods with adequate discriminatory power, reproducibility, high throughput and inter-laboratory comparability are needed for outbreak investigation this complex parasitic disease. As multilocus sequence analysis (MLSA) has been projected as future gold standard technique Leishmania species characterization, we propose a MLSA panel six housekeeping gene loci (6pgd, mpi, icd, hsp70, mdhmt, mdhnc)...
Studies on the sandfly fauna to evaluate natural infection indexes are still limited in Brazilian Amazon, a region with an increasing incidence of cutaneous leishmaniasis. Here, by using multiplex polymerase chain reaction directed Leishmania kDNA and hybridisation, we were able identify L. (Viannia) subgenus 12 out 173 sandflies captured municipality Rio Branco, Acre state, revealing positivity 6.94%. By sequencing 234 bp-hsp70 amplified products from positive samples, (V.) braziliensis was...
Summary The objective of this work was to evaluate the selection immature bovine oocytes by brilliant cresyl blue dye (BCB) and expression transcripts MATER ZAR1. Cumulus–oocyte complexes (COCs) from slaughterhouse ovaries were exposed BCB diluted in mDPBS incubated for 60 min at 38.5 °C humidified air. After exposure those COCs distributed two groups, according their cytoplasm colour: + (coloured cytoplasm) or − (colourless cytoplasm). control group submitted vitro maturation (IVM)...
This study describes the occurrence of dogs naturally co-infected with Hepatozoon canis and two Leishmania species: L. infantum or braziliensis. Four serologically diagnosed Visceral Leishmaniasis were euthanized. Liver spleen samples collected for histopathological analysis DNA isolation. H. meronts observed in tissues from all four dogs. infection was confirmed by PCR followed sequencing a fragment 18S rRNA gene. detection typing ITS1' PCR-RFLP parasite burden calculated using ssrRNA...
Treatment of visceral leishmaniasis in Brazil still relies on meglumine antimoniate, with less than ideal efficacy and safety, making new therapeutic tools an urgent need. The oral drug miltefosine was assayed a phase II clinical trial cure rates lower previously demonstrated India. present study investigated the susceptibility to 73 Brazilian strains Leishmania infantum from different geographic regions, using intracellular amastigote promastigote assays. EC50 for 13 these evaluated...
INTRODUCTION: The aim of this study was quantify annexin A1 expression in macrophages and cluster differentiation 4 (CD4) + 8 (CD8)+ T cells from the skin patients with cutaneous leishmaniasis (n=55) correlate histopathological aspects. METHODS: Infecting species were identified by polymerase chain reaction-restriction fragment length polymorphism, analyzed immunofluorescence. RESULTS: All (n = 55) infected Leishmania braziliensis . Annexin expressed more abundantly CD163 (p < 0.0001) than...
ABSTRACT The Neotropical realm, one of the most biodiverse regions on Earth, houses a broad range zoonoses that pose serious public health threats. Protozoan parasites Leishmania ( Viannia ) braziliensis species complex cause zoonotic leishmaniasis in Latin America with clinical symptoms ranging from simple cutaneous to destructive, disfiguring mucosal lesions. We present first comprehensive genome-wide continental study including 257 cultivated isolates representing geographical...
The main transmission route of Leishmania infantum is through the bites sand flies. However, alternative mechanisms are being investigated, such as ticks, which could have epidemiological relevance. objective this work was to verify presence spp. in Rhipicephalus sanguineus sensu lato collected from naturally infected dogs Federal District Brazil. Ticks were dissected remove their intestines and salivary glands for DNA extraction subsequent amplification conserved region 120 bp kDNA 234...