A5050987045- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Insect Pest Control Strategies
- Parasites and Host Interactions
- Insect and Pesticide Research
- Insect Resistance and Genetics
- Enzyme Production and Characterization
- Tuberculosis Research and Epidemiology
- Plant Virus Research Studies
- Toxin Mechanisms and Immunotoxins
- Synthesis and Biological Evaluation
- Insect symbiosis and bacterial influences
- Biochemical and Molecular Research
- Piperaceae Chemical and Biological Studies
- Eosinophilic Disorders and Syndromes
- Vector-borne infectious diseases
- CRISPR and Genetic Engineering
- Mycobacterium research and diagnosis
- Adolescent Sexual and Reproductive Health
- Zoonotic diseases and public health
- HIV/AIDS Research and Interventions
- RNA modifications and cancer
- Diagnosis and treatment of tuberculosis
- Innovation and Socioeconomic Development
- Lysosomal Storage Disorders Research
Universidad Peruana Cayetano Heredia
2014-2024
Instituto de Medicina Tropical
1985-2021
Public Health Ontario
2015
University Health Network
2015
Toronto General Hospital
2015
Institut de Recherche pour le Développement
2010
Université de Toulouse
2010
Université Toulouse III - Paul Sabatier
2010
Hospital Nacional Dos de Mayo
2009
University of Liverpool
2009
Pentavalent antimonials (SbV) are the first-line chemotherapy for American tegumentary leishmaniasis (ATL). There are, however, reports of occurrence treatment failure with these drugs. Few studies in Latin America have compared response to SbV ATL caused by different Leishmania species.Clinical parameters and were studied 103 patients cutaneous (CL) Peru. isolates collected before typed multilocus polymerase-chain-reaction restriction fragment-length polymorphism analysis.The identified as...
Oligonucleotide primers directed to the minicircle kinetoplast DNA of Leishmania strains supported enzymatic amplification this by polymerase chain reaction (PCR). A single product 70 basepairs was obtained from parasites belonging exclusively L. braziliensis complex. Direct sequencing amplified confirmed its origin. Skin biopsy specimens human patients were used directly for PCR. pulse incubation such with deoxyribonuclease I prior PCR increased reliability assay. Nuclease disruption...
ABSTRACT PCR-restriction fragment length polymorphism analysis of heat shock protein 70 genes discriminates most neotropical Leishmania species, as well Trypanosoma cruzi. The assay, combined with capillary electrophoresis in a microchip device, may be applied directly on clinical samples high sensitivity, hence supporting and epidemiological monitoring leishmaniasis.
Treatment for cutaneous leishmaniasis (CL) with standard pentavalent antimonial therapy is hampered by cumbersome administration, toxicity, and potential failure. Knowledge of factors influencing treatment outcome essential successful management.A case-control study incident cases was performed patients experiencing their first CL episode. The these 20 mg/kg/day sodium stibogluconate days. Clinical epidemiological data were recorded, parasite isolates species typed. Patients followed up 6...
Leishmania species of the subgenus Viannia and especially braziliensis are responsible for a large proportion New World leishmaniasis cases. The reproductive mode has often been assumed to be predominantly clonal, but remains unsettled. We have investigated genetic polymorphism at 12 microsatellite loci on 124 human strains from 2 countries, Peru Bolivia. There is substantial diversity, with an average 12.4 +/- 4.4 alleles per locus. linkage disequilibrium genome-wide scale, as well...
Cutaneous and mucosal leishmaniasis, caused in South America by Leishmania braziliensis, is difficult to cure chemotherapy (primarily pentavalent antimonials [Sb(V)]). Treatment failure does not correlate well with resistance vitro, the factors responsible for treatment patients are understood. Many isolates of L. braziliensis (>25%) contain a double-stranded RNA virus named Leishmaniavirus 1 (LRV1), which has also been reported guyanensis, an association increased pathology, metastasis,...
Earlier histopathology studies suggest that parasite loads may differ between cutaneous leishmaniasis (CL) and mucosal (ML) lesions acute chronic CL. Formal demonstration requires highly sensitive detection accurate quantification of Leishmania in human lesional tissue. In this study, we developed a quantitative real-time PCR (qPCR) assay targeting minicircle kinetoplast DNA (kDNA) to detect quantify (Viannia) parasites. We evaluated total 156 lesion biopsy specimens from CL or ML suspected...
Significance Parasites are interesting models for studying speciation processes because they have a high potential specialization, thanks to the intimate ecological association with their hosts and vectors. Yet little is known about circumstances under which new parasite lineages emerge. Here we studied genome diversity of parasites Leishmania braziliensis species complex that inhabit both Amazonian Andean biotas in Peru. We identify three major occupy particular niches show these emerged...
Antimonials are the first drug of choice for treatment American tegumentary leishmaniasis (ATL); however, their efficacy is not predictable, and this may be linked to parasite resistance. We aimed characterize in vitro antimony susceptibility clinical isolates Peruvian patients with ATL who were treated sodium stibogluconate correlate phenotype different outcomes.Thirty-seven obtained from known disease histories. These typed, intracellular amastigotes pentavalent (SbV) trivalent (SbIII)...
Journal Article Natural Leishmania infection of Lutzomyia spp. in Peru Get access J. Enrique Perez, Perez 1Instituto de Medicina Tropical 'Alexander von Humboldt' Universidad Peruana Cayetano Heredia, Apartado 4314, Lima-100, Perú3Direccíon Investigación Epidemiológica, Instituto Enfermedades Transmisibles 'Dr Hugo Lumbreras Cruz', Nacional Salud, Lima, Perú Address for correspondence: Pérez, Humboldt', Perú. Search other works by this author on: Oxford Academic PubMed Google Scholar Elena...
Background. Definite diagnosis of Leishmania infections is based on demonstration the parasite by microscopic analysis tissue biopsy specimens or aspirate samples. However, microscopy generally shows low sensitivity and requires invasive sampling.
Traditional detection of Leishmania from ulcers involves collection invasive specimens that cause discomfort, require technical expertise, and carry risks procedures. We compared traditional diagnostic methods with a molecular noninvasive filter paper-based method for the diagnosis cutaneous leishmaniasis.Consecutive patients presenting to Clinic at Hospital Nacional Cayetano Heredia were enrolled. Polymerase chain reaction (PCR) was performed on lesion scrapings, aspirates, paper...
Tegumentary leishmaniasis, a disease caused by protozoan parasites of the genus Leishmania , is major public health problem in many regions Latin America. Its diagnosis difficult given other conditions resembling leishmaniasis lesions and co-occurring same endemic areas. A combination parasitological molecular methods leads to accurate diagnosis, with latter being traditionally performed centralized reference research laboratories as they require specialized infrastructure operators....
In the present study gp63 gene locus was used as a target for genetic characterization of Leishmania parasites by 2 methods: (i) RFLP analysis with several restriction enzymes (gp63-RFLP), and (ii) intra-genic PCR amplification coupled (PCR-RFLP). Both methods were applied to large number natural isolates belonging 4 species subgenus Viannia, namely L. (V.) braziliensis, peruviana, guyanensis lainsoni; reference stocks included outgroups. Multilocus isoenzyme typing (MLEE) reference. On one...
In resource-poor settings, the mental health burden among HIV-positive women is exacerbated by poverty. We sought to describe extent, risk factors, and experience of depression impoverished living in Lima, Peru.This a case series 78 Peru. measured depression, stigma, social support performed multivariable analysis identify factors associated with depression.Among patients, 68% were depressed. Depression suicidal ideation rarely diagnosed providers. analysis, HIV-related stigma food scarcity...
Leishmania (subgenus Viannia) braziliensis is the causative agent of mucocutaneous leishmaniasis (ML) in South America, and ML characterized by excessive T- B-cell responses to parasite. We speculate that unbalanced production inflammatory mediators response L. infection contributes cell recruitment disease severity. To test this hypothesis, we first examined peripheral blood mononuclear cells (PBMCs) from healthy volunteers infection. observed while induced chemokine (C-X-C motif) ligand 10...