A5084483359- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Synthesis and Biological Evaluation
- Insect Pest Control Strategies
- Piperaceae Chemical and Biological Studies
- Insect Utilization and Effects
- Toxin Mechanisms and Immunotoxins
- Helicobacter pylori-related gastroenterology studies
- Animal and Plant Science Education
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Antimicrobial Peptides and Activities
- Natural product bioactivities and synthesis
- Veterinary medicine and infectious diseases
- Helminth infection and control
- Innovative Microfluidic and Catalytic Techniques Innovation
- Traditional and Medicinal Uses of Annonaceae
- 3D Printing in Biomedical Research
- Beetle Biology and Toxicology Studies
- Additive Manufacturing and 3D Printing Technologies
- Biological Activity of Diterpenoids and Biflavonoids
- Plant Toxicity and Pharmacological Properties
- Bioactive Natural Diterpenoids Research
- Plant-derived Lignans Synthesis and Bioactivity
- Microscopic Colitis
Universidad Peruana Cayetano Heredia
2011-2024
Amazon (United States)
2019
Centro de Investigación y Desarrollo
2019
Instituto de Medicina Tropical
2011
Université de Toulouse
2010
Université Toulouse III - Paul Sabatier
2010
Institut de Recherche pour le Développement
2010
It was recently hypothesized that Leishmania amastigotes could constitute a semi-quiescent stage characterized by low replication and reduced metabolic activity. This concept developed with (Leishmania) mexicana major models might explain numerous clinical sub-clinical features of (Viannia) braziliensis infections, like reactivation the disease, non-response to chemotherapy or asymptomatic infections. We compared here in vitro proliferative capability L. (V.) promastigotes, assessed...
Abstract Helicobacter pylori (Marshall & Goodwin) is a widespread human pathogen that acquiring resistance to the antibiotics used treat it. This increasing necessitates continued search for new antibiotics. An antibiotic source shows promise animals whose immune systems must adapt living in bacteria-laden conditions by producing antibacterial peptides or small molecules. Among these black soldier fly (BSF; Hermetia illucens Linnaeus), Diptera colonizes decomposing organic matter. In...
Three new caffeic acid esters (1−3), four lignans (4−7), and the known compounds (7′S)-parabenzlactone (8), dihydrocubebin (9), justiflorinol (10) have been isolated from leaves of Piper sanguineispicum. Their structures were determined by spectroscopic methods, including 1D 2D NMR, HRCIMS, CD experiments, chemical methods. Compounds 1−10 assessed for their antileishmanial potential against axenic amastigote forms Leishmania amazonensis. Caffeic 1 3 exhibited best activity (IC50 2.0 1.8 μM,...
The venom peptide bicarinalin, previously isolated from the ant Tetramorium bicarinatum, is an antimicrobial agent with a broad spectrum of activity. In this study, we investigate potential bicarinalin as novel against Helicobacter pylori, which causes several gastric diseases. First, effects synthetic have been tested pylori: one ATCC strain, and forty-four stomach ulcer biopsies Peruvian patients. Then cytoxicity on human cells murine peritoneal macrophages was measured using XTT MTT...
Seven benzo[c]phenanthridines, synthetic or isolated from Zanthoxylum rhoifolium root bark, were evaluated against Leishmania amazonensis axenic amastigotes. Five of them considered leishmanicidal, with IC50 values ranging 0.03 to 0.54 µM, and on intramacrophagic amastigotes L. amazonensis. Chelerythrine displayed the best activity (IC50=0.5 µM), which was in same range as reference compound amphotericin B (IC50=0.4 µM). In vivo studies chelerythrine, avicine, fagaridine a model mice...
Two new dihydrochalcones (<b>1, 2</b>), as well eight known compounds, piperaduncin C (<b>3</b>), 2′,6′-dihydroxy-4′-methoxydihydrochalcone (<b>4</b>), 4,2′,6′-trihydroxy-4′-methoxydihydrochalcone (<b>5</b>), 4-hydroxy-3,5-bis(3-methyl-2-butenyl)-benzoic acid (<b>6</b>), 3,5-bis(3-methyl-2-butenyl)-4-methoxybenzoic (<b>7</b>), 4-hydroxy-3-(3-methyl-2-butenoyl)-5-(3-methyl-2-butenyl)-benzoic (<b>8</b>), 2,2-dimethyl-8-(3-methyl-2-butenyl)-2<i>H</i>-1-chromene-6-carboxylic (<b>9</b>), and...
We explored the clinical-stage association of gastric intestinal metaplasia (IM) compared to cases chronic non-atrophic gastritis (CNAG) and its relationship with virulence genotypes Helicobacter pylori (H. pylori) clinical isolates from patients dyspepsia in Peru. This study was cross-sectional included 158 H. isolates; each isolate corresponded a different Peruvian patient, genotyped by polymerase chain reaction detect cagA gene EPIYA motifs, vacA (alleles s1, s2, i1, i2, d1, d2, m1, m2...
Background Most of the Leishmania genome is reported to be constitutively expressed during life cycle parasite, with a few regulated genes. Inter-species comparative transcriptomics evidenced low number species-specific differences related differentially distributed genes or differential regulation conserved It uppermost importance ensure that observed are indeed and not simply specific strains selected for representing species. The relevance this concern illustrated by current study....
The gut microbiota of insects is composed a wide range microorganisms which produce bioactive compounds that protect their host from pathogenic attack. In the present study, we isolate and identify fungus Chrysosporium multifidum Hermetia illucens larvae. Extract C. culture broth supernatant showed moderate activity against strain methicillin-resistant Staphylococcus aureus (MRSA). Bioguided isolation extract resulted in characterization six α-pyrone derivatives (1–6) one diketopiperazine...
A series of ring substituted 3-phenyl-1-(1,4-di-N-oxide quinoxalin-2-yl)-2-propen-1-one derivatives were synthesized and tested for in vitro leishmanicidal activity against amastigotes Leishmania amazonensis axenical cultures murine infected macrophages. Structure-activity relationships demonstrated the importance a radical methoxy at position R3', R4' R5'. (2E)-3-(3,4,5-trimethoxy-phenyl)-1-(3,6,7-trimethyl-1,4-dioxy-quinoxalin-2-yl)-propenone was most active. Cytotoxicity on macrophages...
An anticancer-bioassay guided isolation of the ethanol extract and fractions two plants from Peruvian rainforest, Mikania decora Cremastosperma microcarpum, led to characterization one abundant diterpene, ent-pimara-8(14),15-dien-19-oic acid (1), three thymol derivatives, 10-acetoxy-8,9-dehydro-6-methoxythymol butyrate (2), 10-acetoxy-8,9-epoxy-6-methoxythymol isobutyrate (3), acetylschizoginol (4), as well neolignan, (±)-trans-dehydrodiisoeugenol (5). Only latter was isolated C....
The phytochemistry of the latex Hura crepitans L. (Euphorbiaceae), a widespread tree in Amazonian forest having many uses, is little known. Only huratoxin, daphnane diterpene orthoester, has been described despite high pharmacological potential this kind compounds. Glucosphingolipids (cerebrosides) are also known to be distributed Euphorbiaceae latexes.To tentatively identify daphnanes diterpenes and cerebrosides H. crepitans.An ethanolic extract lyophilised was analysed by...
Six new secocycloartane glycosides (1–6) were isolated from the ethanol extract of flowers Cordia lutea Lam. on basis bioassay-guided fractionation. Their structures determined by application NMR and MS data analyses together with X-ray crystallographic for compounds 1 2. Compounds 1–6 represent first examples 9,10-seco-29-norcycloartane glycosides. These showed significant in vitro anti-Helicobacter pylori activity, no activity against either Escherichia coli or Pseudomonas aeruginosa....
One new phthalide (1) was isolated from aerial parts of Peperomia nivalis, along with known compounds (2 and 3), reported in this species for the first time. The structure compound characterised on basis 1D (1H 13C NMR), 2D (COSY, HMQC, HMBC NOESY) NMR high-resolution mass spectral (HRMS) data. Compound 2 a natural source time but previously synthesised. Compounds 1–3 were evaluated their anti-Helicobacter pylori anti-Plasmodium falciparum activities. 1 showed moderate activities against H....
In order to evaluate antimicrobial susceptibility and detect specific mutations in the 23S rRNA gene Helicobacter pylori strains, a cross-sectional study was performed on 95 patients with dyspepsia treated private clinic Lima. Antrum biopsies were collected by endoscopy for isolation evaluation of using broth microdilution method. The detection developed PCR-RFLP. percentage infection 46.3%. Resistance values 52.3% clarithromycin, 29.6% metronidazole, 45.5% levofloxacin, 4.6% amoxicillin...