A5014096329- Trypanosoma species research and implications
- Vector-borne infectious diseases
- Research on Leishmaniasis Studies
- Insect symbiosis and bacterial influences
- Viral Infections and Vectors
- Leptospirosis research and findings
- Bat Biology and Ecology Studies
- Vector-Borne Animal Diseases
- Parasites and Host Interactions
- Parasitic Infections and Diagnostics
- Quantum Information and Cryptography
- Quantum Computing Algorithms and Architecture
- Adventure Sports and Sensation Seeking
- Rabies epidemiology and control
- Sport and Mega-Event Impacts
- Bartonella species infections research
- Toxoplasma gondii Research Studies
- Recreation, Leisure, Wilderness Management
- Yersinia bacterium, plague, ectoparasites research
- Insect and Pesticide Research
- Zoonotic diseases and public health
- Brazilian Legal Issues
- Insect Utilization and Effects
- Bird parasitology and diseases
- Brucella: diagnosis, epidemiology, treatment
Fundação Oswaldo Cruz
2016-2025
Instituto Politécnico de Lisboa
2024
University of Lisbon
2024
Direção Geral do Património Cultural
2012
University of Coimbra
2012
We report Trypanosoma cruzi infection in wild and domestic mammals from three orally acquired Chagas disease outbreak areas Brazil. Cachoeiro do Arari (Pará) displayed a panzootic scenery (positive all ecologic strata), human cases were probably the consequence of their exposure within sylvatic T. transmission cycle. In Navegantes (Santa Catarina), Didelphis spp. was main reservoir host, given that 93% infected. Redenção (Ceará), Monodelphis domestica Thrichomys laurentius also important...
Little is known on the role played by Neotropical wild carnivores in Trypanosoma cruzi transmission cycles. We investigated T. infection from three sites Brazil through parasitological and serological tests. The seven carnivore species examined were infected cruzi, but high parasitemias detectable hemoculture found only two Procyonidae species. Genotyping Mini-exon gene, PCR-RFLP (1f8/Akw21I) kDNA genomic targets revealed that raccoon (Procyon cancrivorus) harbored TcI coatis (Nasua nasua)...
A new epidemiological scenario involving the oral transmission of Chagas disease, mainly in Amazon basin, requires innovative control measures. Geospatial analyses Trypanosoma cruzi cycle wild mammals have been scarce. We applied interpolation and map algebra methods to evaluate mammalian fauna variables related small T. infection pattern dogs identify hotspot areas transmission. also evaluated use as sentinels risk disease. Dogs (n = 649) were examined by two parasitological three distinct...
The new epidemiological scenario of orally transmitted Chagas disease that has emerged in Brazil, and mainly the Amazon region, needs to be addressed with a systematic focus. Belém, capital Pará state, reports highest number acute (ACD) cases associated consumption açaí juice.The wild domestic enzootic transmission cycles Trypanosoma cruzi were evaluated two locations (Jurunas Val-de Cães) report majority autochthonous ACD Belém city. Moreover, we cycle on three islands provide most fruit is...
Bats have a long evolutionary history with trypanosomatids, but the role of these flying mammals on parasite transmission cycles in urban areas, especially for Trypanosoma and Leishmania species, remains poorly known. The objective this study was to evaluate species richness trypanosomatids parasitizing bat community Campo Grande (CG), state capital within Cerrado Brazilian Midwest. We evaluated 237 bats 13 by means hemoculture molecular detection spleen samples. CG appears participate...
SUMMARY Aiming to better understand the ecological aspects of Trypanosoma cruzi transmission cycles, wild carnivores, small mammals and dogs were examined for T. infection in Serra da Canastra National Park region, Brazil. Isolates genotyped using mini-exon gene PCR-RFLP (1f8 H3) genomic targets. was well established area occurred both peridomestic environments. Dog seroprevalence 29·4% (63/214) TcI TcII genotypes, besides mixed infections observed. Only detected mammals. Marsupials...
Trypanosoma cruzi infection via oral route results in outbreaks or cases of acute Chagas disease (ACD) different Brazilian regions and poses a novel epidemiological scenario. In the Espírito Santo state (southeastern Brazil), fatal case patient with ACD led us to investigate enzootic scenario avoid development new cases. At studied locality, Triatoma vitticeps exhibited high T. rates frequently invaded residences. Sylvatic domestic mammals Rio da Prata where occurred, four surrounding areas...
Trypanosoma spp. infection in wild mammals is detected mainly through parasitological tests that usually display low sensitivity. We propose the use of DNA extracted directly from blood clots (BC), which are neglected sources for diagnosis and identification This approach followed by nested PCR targeting 18S SSU rDNA demonstrated to be sensitive suitable evaluate diversity trypanosomes infecting sylvatic mammals, including subpatent mixed infections. Infection was 95/120 (79.2%) samples...
ABSTRACT Bartonella spp. comprise an ecologically successful group of microorganisms that infect erythrocytes and have adapted to different hosts, which include a wide range mammals, besides humans. Rodents are reservoirs about two-thirds described date; some them been implicated as causative agents human diseases. In our study, we performed molecular phylogenetic analyses infecting wild rodents from five Brazilian biomes. order characterize the genetic diversity spp., robust analysis based...
The present study aimed to assess Trypanosoma spp. infection in 17 captive black-faced black spider monkeys ( Ateles chamek ), living at the Zoological Garden of Curitiba, Paraná, Brazil. Blood samples (3 mL) were collected by femoral puncture certified veterinarians. A total 5 μL extracted blood DNA was submitted Nested-PCR 18S rDNA. In overall, 4/17 (23.5%) positive PCR. Sequencing results for rDNA revealed minasense infection. This is first report T. monkey worldwide and assessment...
Introduction Trypanosomatids are parasites widely distributed in nature, parasitizing several host species single or co-infections. Campo Grande (CG), capital of Mato Grosso do Sul State, is characterized by green areas and forest fragments where wild mammals have been reported infected diverse trypanosomatid species. In this study, we evaluated the parasitism trypanosomatids non-human primates (NHP) Sapajus cay Alouatta caraya sampled three different CG. Material methods For detection...
The presence of acute Chagas disease (ACD) due to oral transmission is growing and expanding in several South American countries. Within the Amazon basin, Abaetetuba municipality has been a site recurrent cases spanning across distinct landscapes. Because primarily zoonotic infection, we compared enzootic Trypanosoma cruzi cycles three different environmental areas better understand this new epidemiological situation. Philander opossum was most abundant mammalian species collected (38%...
The aim of this study was to reevaluate the ecology an area in Atlantic Forest, southeast Brazil, where Chagas disease (CD) has been found occur. In a previous study, immediately after occurrence CD case, we did not observe any sylvatic small mammals or dogs with Trypanosoma cruzi infections, but Triatoma vitticeps presented high T. c. infection rates. investigated bats together non-volant mammals, dogs, and triatomines explore other possible reservoirs/hosts area. Seventy-three 186 were...
SUMMARY Some Trypanosoma and Leishmania species are multi-host parasites whose distribution overlaps in several parts of the Brazilian Amazon basin. Despite being a common trait among wild mammals, mixed infections their consequences for host's health parasite transmission still poorly known phenomenon. Here we describe triple infection – cruzi , T. rangeli infantum bone marrow sample from an anteater Tamandua tetradactyla captured house backyard endemic Abaetetuba municipality was also...
Abstract Bats are ancient hosts of Trypanosoma species and their flying ability, longevity adaptability to distinct environments indicate that they efficient dispersers parasites. from Acre state (Amazon Biome) were collected in four expeditions conducted an urban forest (Parque Zoobotânico) one relatively more preserved area (Seringal Cahoeira) Rio Branco Xapuri municipalities. sp. infection was detected by hemoculture fresh blood examination. Isolated parasite identified the similarity...
Trypanosoma cruzi infection is a complex sylvatic enzooty involving wide range of animal species. Six discrete typing units (DTUs) T. cruzi, named TcI to TcVI, are currently recognized. One unanswered question concerning the epidemiology distribution pattern TcII and hybrid DTUs in nature, including their virtual absence Brazilian Amazon, current endemic area Chagas disease Brazil. Herein, we characterized biological samples that were collected previous epizootiological studies carried out...
Background Caviomorph rodents, some of the oldest Leishmania spp. hosts, are widely dispersed in Brazil. Despite both experimental and field studies having suggested that these rodents potential reservoirs parasites, not more than 88 specimens were analyzed few natural infection. Our hypothesis was caviomorph inserted transmission cycles different regions, so is currently recognized. Methodology We investigated infection spleen fragments 373 from 20 species collected five Brazilian biomes a...
After the control of main modes Chagas disease (CD) transmission in most endemic countries, it is important to identify participation native sylvatic vectors CD transmission. Although not considered Rio de Janeiro State (RJ), Brazil, we identified patients with born RJ and investigated possible autochthonous state. Patients followed our institution between 1986 2011 were retrospectively analyzed. The cases as submitted epidemiological, clinical, serological, parasitological molecular...
We studied infection by Trypanosomatidae in bats captured two areas with different degradation levels the Atlantic Forest of Rio de Janeiro state: Reserva Ecológica Guapiaçu (REGUA) and Estação Fiocruz Mata Atlântica (EFMA). Furthermore, we evaluated whether diversity trypanosomatids changes according to bat preservation region. The results showed no influence level on species richness (15 14 species, respectively), similar chiropterofauna higher abundance common fruit-eating tropics:...
Crithidia mellificae, a monoxenous trypanosomatid considered restricted to insects, was recently reported infect bat. Herein, C. mellificae has been demonstrated have wider range of vertebrate hosts and distribution in Brazilian biomes than once thought. Parasites isolated from haemocultures were characterized using V7V8 SSU rDNA glyceraldehyde 3-phosphate dehydrogenase genes. Coatis (Nasua nasua) the Cerrado; marmosets (Callithrix sp.) bats (Carollia perspicillata, Myotis lavali, M....