A5077108545- Rheumatoid Arthritis Research and Therapies
- Systemic Lupus Erythematosus Research
- Musculoskeletal pain and rehabilitation
- Pain Mechanisms and Treatments
- Pharmaceutical studies and practices
- Sulfur Compounds in Biology
- Pain Management and Treatment
- Health Systems, Economic Evaluations, Quality of Life
- Receptor Mechanisms and Signaling
- Redox biology and oxidative stress
- Neuropeptides and Animal Physiology
- Autoimmune and Inflammatory Disorders Research
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Spine and Intervertebral Disc Pathology
- Cytokine Signaling Pathways and Interactions
- Transcranial Magnetic Stimulation Studies
- Context-Aware Activity Recognition Systems
- Anesthesia and Pain Management
- IoT-based Smart Home Systems
- Dermatological and Skeletal Disorders
- Peripheral Neuropathies and Disorders
- Mast cells and histamine
- Adolescent and Pediatric Healthcare
- Heterotopic Ossification and Related Conditions
- Gout, Hyperuricemia, Uric Acid
Royal Devon & Exeter NHS Foundation Trust
2008-2021
Royal Devon and Exeter Hospital
2008-2020
University of Exeter
2010-2019
Princess Elizabeth Orthopaedic Centre
2008-2018
Peninsula College of Medicine and Dentistry
2006-2014
University of Otago
2012
Heavitree Hospital
2011
Peninsula Health
2010
Royal National Hospital for Rheumatic Diseases
1986-2008
Nottingham City Hospital
2008
To determine whether MPO contributes to oxidative stress and disease activity in RA it produces hypochlorous acid SF.Plasma where possible SF were collected from 77 patients while 120 healthy controls supplied plasma only. protein carbonyls measured by ELISAs. 3-Chlorotyrosine proteins allantoin mass spectrometry.Plasma concentrations significantly higher with compared [10.8 ng/ml, inter-quartile range (IQR): 7.2-14.2; P<0.05], but there was no significant difference between high (HDA;...
Hydrogen sulfide (H(2)S) has recently been proposed as an endogenous mediator of inflammation and is present in human synovial fluid. This study determined whether primary articular chondrocytes (HACs) mesenchymal progenitor cells (MPCs) could synthesize H(2)S response to pro-inflammatory cytokines relevant arthropathies, determine the cellular responses pharmacological H(2)S. HACs MPCs were exposed IL-1β, IL-6, TNF-α lipopolysaccharide (LPS). The expression enzymatic activity synthesizing...
Abstract There is currently no biochemical test for detection of early-stage osteoarthritis (eOA). Tests rheumatoid arthritis (eRA) such as factor (RF) and anti–cyclic citrullinated peptide (CCP) antibodies require refinement to improve clinical utility. We developed robust mass spectrometric methods quantify protein (CP) free hydroxyproline in body fluids. detected CP the plasma healthy subjects surprisingly found that was increased both patients with eOA eRA whereas anti–CCP were...
Blood concentrations of hydrogen sulfide (H 2 S) are markedly elevated in several animal models inflammation. Pharmacological inhibition H S synthesis reduces inflammation and swelling, suggesting that is a potential inflammatory mediator. However, it currently unknown whether perturbed human conditions or present synovial fluid. We analyzed paired plasma fluid (SF) aspirates from rheumatoid arthritis (RA; n = 20) osteoarthritis (OA; 4) patients age matched healthy volunteers ( 20). Median...
Rheumatoid arthritis (RA) is considered a T cell driven autoimmune disease, therefore, the ability of B depleting biologics, e.g., anti-CD20 antibodies, to alleviate RA unclear. This study examined proportions IL-17-secreting lymphocytes in blood healthy subjects and patients determined if Th17 cells belong CD20+ subset cells. Fluorescence-activated sorting confocal microscopy verified CD3, CD4/CD8 CD20-staining IL-17 secretion was using commercial assay. In blood, median percentage total...
Abstract Complex regional pain syndrome (CRPS) is a persistent condition that remains incompletely understood and challenging to treat. Historically, wide range of different outcome measures have been used capture the multidimensional nature CRPS. This has significant limiting factor in advancement our understanding mechanisms management In 2013, an international consortium patients, clinicians, researchers, industry representatives was established, develop agree on minimum core set...
Contralateral responses to unilateral stimuli have been well described in animal models. These range from central sensitization peripheral inflammatory responses. Our aim was test for contralateral following intradermal capsaicin injection man.Three groups were investigated. A healthy volunteer group (1) injected with into the volar aspect of one forearm. patients RA (2) also capsaicin. control volunteers (3) not All tested hyperalgesia and allodynia every 10 min 1 h using quantitative...
Abstract Objective The binding of FasL (CD95L) to its receptor, Fas (CD95), induces apoptosis. Studies have shown that in patients with rheumatoid arthritis (RA), T lymphocytes are resistant FasL‐induced apoptosis vivo but susceptible vitro. Dysfunction this mechanism may be an important contributor the pathophysiology RA. Thus, present study was undertaken determine which factors might inhibit FasL–Fas and those would vitro model system. Methods Human Jurkat cells rendered apoptotic by...
Peroxiredoxin 2 has immune regulatory functions, but its expression in human peripheral blood lymphocytes and levels extracellular fluid healthy subjects rheumatoid arthritis patients are poorly described. In the present study, median intracellular peroxiredoxin protein content of from was more than two-fold higher compared with subjects' lymphocytes. Intracellular 3 were similar Flow cytometry detected on surface ca. 8% T cells 56% B (median % values) all analyzed. Exofacial thioredoxin-1...
Objective. The nature and cause of perceived joint stiffness (PJS), a well‐established defining symptom rheumatoid arthritis (RA), remains unclear. We hypothesized that changes in the central nervous system (CNS) may determine maintain this subjective experience limb even after it is amputated. To test hypothesis, patients with phantom (PL) who had experienced characteristic RA prior to amputation were systematically investigated.
Protein fractionation is a useful separation process which divides membrane proteins (including those located in the outer and inner membrane) cytoplasmic into discrete fractions. Fractionation of can simplify analysis numbers present, therefore make easier to characterize any environmentally or mutation induced changes expression profiles, protein strucutre resulting from post-translational modification. This protocol derived Haigh et al. (2013) it specific Gram negative bacteria.
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