A5021086938- Advanced Glycation End Products research
- Biochemical effects in animals
- Alcohol Consumption and Health Effects
- Alcoholism and Thiamine Deficiency
- Genomics, phytochemicals, and oxidative stress
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Neurological and metabolic disorders
- Neurological Disorders and Treatments
- Metabolomics and Mass Spectrometry Studies
- Natural Antidiabetic Agents Studies
- Biochemical Acid Research Studies
- Rheumatoid Arthritis Research and Therapies
- Muscle metabolism and nutrition
- Chronic Kidney Disease and Diabetes
- Protein Hydrolysis and Bioactive Peptides
- Diet and metabolism studies
- Osteoarthritis Treatment and Mechanisms
- Diabetes Management and Research
- Metabolism, Diabetes, and Cancer
- Metabolism and Genetic Disorders
- Adipose Tissue and Metabolism
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Pancreatic function and diabetes
- Liver Disease Diagnosis and Treatment
- Glutathione Transferases and Polymorphisms
Qatar University
2020-2024
Coventry (United Kingdom)
2019-2021
University of Warwick
2011-2020
University Hospital Coventry
2011-2020
Institute for Systems Biology
2019
Czech Academy of Sciences, Biology Centre
2017
RELX Group (United States)
2016
University Hospitals Coventry and Warwickshire NHS Trust
2015
University of Essex
2007-2010
University Hospital Southampton NHS Foundation Trust
2010
OBJECTIVE Establishing Caenorhabditis elegans as a model for glucose toxicity–mediated life span reduction. RESEARCH DESIGN AND METHODS C. were maintained to achieve concentrations resembling the hyperglycemic conditions in diabetic patients. The effects of high on span, glyoxalase-1 activity, advanced glycation end products (AGEs), and reactive oxygen species (ROS) formation mitochondrial function studied. RESULTS High reduced mean from 18.5 ± 0.4 16.5 0.6 days maximum 25.9 23.2 days,...
Abnormal cellular accumulation of the dicarbonyl metabolite MG (methylglyoxal) occurs on exposure to high glucose concentrations, inflammation, cell aging and senescence. It is associated with increased MG-adduct content protein DNA linked strand breaks mutagenesis, mitochondrial dysfunction ROS (reactive oxygen species) formation detachment from extracellular matrix. MG-mediated damage countered by glutathione-dependent metabolism Glo1 (glyoxalase 1). not known, however, whether has...
Age-related macular degeneration (AMD) is the major cause of blindness in developed nations. AMD characterized by retinal pigmented epithelial (RPE) cell dysfunction and loss photoreceptor cells. Epidemiologic studies indicate important contributions dietary patterns to risk for AMD, but mechanisms relating diet disease remain unclear. Here we investigate effect on isocaloric diets that differ only type carbohydrate a wild-type aged-mouse model. The consumption high-glycemia (HG) resulted...
Risk of insulin resistance, impaired glycemic control, and cardiovascular disease is excessive in overweight obese populations. We hypothesized that increasing expression glyoxalase 1 (Glo1)—an enzyme catalyzes the metabolism reactive metabolite glycating agent methylglyoxal—may improve metabolic vascular health. Dietary bioactive compounds were screened for Glo1 inducer activity a functional reporter assay, hits confirmed cell culture, an optimized formulation was evaluated randomized,...
To assess thiamine status by analysis of plasma, erythrocytes and urine in type 1 2 diabetic patients links to markers vascular dysfunction.Diabetic (26 48 2) with without microalbuminuria 20 normal healthy control volunteers were recruited. Erythrocyte activity transketolase, the concentrations related phosphorylated metabolites urine, metabolic dysfunction determined.Plasma concentration was decreased 76% 75% patients: 64.1 (95% CI 58.5-69.7) nmol/l, diabetes 15.3 11.5-19.1) p < 0.001,...
Sulforaphane is an activator of transcription factor NF-E2-related factor-2 (nrf2) that regulates gene expression through the promoter antioxidant response element (ARE). Nrf2 a battery protective and metabolic enzymes. The aim this study was to assess whether activation nrf2 by sulforaphane in human microvascular endothelial cells prevents dysfunction hyperglycemia.Human HMEC-1 were incubated low high glucose concentrations (5 30 mmol/l, respectively), assessed nuclear translocation....
OBJECTIVE The goal of this study was to characterize glycation adducts formed in both vivo extracellular matrix (ECM) proteins endoneurium from streptozotocin (STZ)-induced diabetic rats and vitro by laminin fibronectin with methylglyoxal glucose. We also investigated the impact advanced end product (AGE) residue content ECM on neurite outgrowth sensory neurons. RESEARCH DESIGN AND METHODS Glycation, oxidation, nitration extracted control STZ-induced rat sciatic nerve (3–24 weeks post-STZ)...
Protection of mitochondrial proteins from glycation by endogenous dicarbonyl compounds, methylglyoxal and glyoxal, was found recently to prevent increased formation reactive oxygen species oxidative nitrosative damage the proteome during aging produce life extension in nematode Caenorhabditis elegans. This suggests that may be a preceding event dysfunction leading stress. Future research will address functional charges are targets for glycation.
OBJECTIVE To study whether modification of LDL by methylglyoxal (MG), a potent arginine-directed glycating agent that is increased in diabetes, associated with atherogenicity. RESEARCH DESIGN AND METHODS Human was isolated and modified MG vitro to minimal extent (MGmin-LDL) as occurs vivo. Atherogenic characteristics MGmin-LDL were characterized: particle size, proteoglycan-binding, susceptibility aggregation, non-LDL receptor–binding, aortal deposition. The major site apolipoprotein B100...