A5009104296- Thyroid Disorders and Treatments
- Pituitary Gland Disorders and Treatments
- Advanced Glycation End Products research
- Diet and metabolism studies
- Metabolism, Diabetes, and Cancer
- Selenium in Biological Systems
- Metabolism and Genetic Disorders
- Gut microbiota and health
- Diabetes and associated disorders
- Adipose Tissue and Metabolism
- Vitamin D Research Studies
- Thyroid Cancer Diagnosis and Treatment
- Neuroendocrine Tumor Research Advances
- Neurological Disorders and Treatments
- Growth Hormone and Insulin-like Growth Factors
- Biochemical effects in animals
- Genetic Associations and Epidemiology
- MicroRNA in disease regulation
- Alcohol Consumption and Health Effects
- Epigenetics and DNA Methylation
- Natural Antidiabetic Agents Studies
- Neonatal Health and Biochemistry
- Sleep and related disorders
- Circadian rhythm and melatonin
- Immunodeficiency and Autoimmune Disorders
Riga East University Hospital
2015-2024
Riga Stradiņš University
2015-2024
Latvian Biomedical Research and Study Centre
2019-2024
University of Latvia
2017
Heidelberg University
2005-2012
University Hospital Heidelberg
2005-2012
Latvijas Organiskās Sintēzes Institūts
2012
Recent studies have revealed strong associations between systemic trimethylamine N-oxide (TMAO) levels, atherosclerosis and cardiovascular risk. In addition, plasma L-carnitine levels in patients with high TMAO concentrations predicted an increased risk for disease incident major adverse cardiac events. The aim of the present study was to investigate relation diabetes. Blood samples were collected from 12 20 weeks old db/db mice undergoing percutaneous coronary intervention. Diabetic...
Abstract The disclosure of proven cardiorenal benefits with certain antidiabetic agents was supposed to herald a new era in the management type 2 diabetes (T2D), especially for many patients T2D who are at high risk cardiovascular and renal events. However, as evidence favour various sodium–glucose transporter-2 inhibitor (SGLT2i) glucagon-like peptide-1 receptor agonists (GLP-1 RA) accumulates, prescriptions these continue stagnate, even among eligible, at-risk patients. By contrast,...
Background Metformin is a widely used first-line drug for treatment of type 2 diabetes. Despite its advantages, metformin has variable therapeutic effects, contraindications, and side effects. Here, the very first time, we investigate short-term effect on composition healthy human gut microbiota. Methods We an exploratory longitudinal study design in which sample from individual was control further samples. Eighteen individuals were treated with (2 × 850 mg) 7 days. Stool samples collected...
Abstract BACKGROUND The health‐promoting properties of apples are directly related to the biologically active compounds that they contain, such as polyphenols. objective this study was prepare a low‐sugar, fibre‐ and phlorizin‐enriched powder from unripe gain insight regarding its anti‐hyperglycaemic activity in healthy volunteers. RESULTS ( Malus domestica Borkh.) were collected 30 days after full bloom day; blanched pressed obtain apple pomace which then processed with food cutter,...
The study was conducted to investigate the effects of metformin treatment on human gut microbiome's taxonomic and functional profile in Latvian population, evaluate correlation these changes with therapeutic efficacy tolerance.In this longitudinal observational study, stool samples for shotgun metagenomic sequencing-based analysis were collected two cohorts. first cohort included 35 healthy nondiabetic individuals (metformin dose 2x850mg/day) at three time-points during administration....
Autoimmune thyroid disease (AITD), including Graves' (GD) or Hashimoto's thyroiditis (HT), occurs due to genetic susceptibility and environmental factors, among which the role of stressful events remains controversial. This study investigated relationship between number impact life in AITD patients with selenium status, Th1/Th2/Th17 immune response. The population included three groups: HT (n = 47), GD 13), a control group 49). Thyroid function parameters, autoantibody levels, plasma levels...
CD34+/CD133+ circulating adult progenitor cells (PCs) play an important role in tissue repair metabolic disease. PCs have a certain plasticity to differentiate into with tissue-specific phenotypes (1–3). In diabetic mice, therapeutic use of models hindlimb ischemia and wound healing was particularly effective, suggesting diabetes-dependent defect PC function (4,5). Accordingly, vitro outgrowth angiogenic endothelial differentiating from (1) is diminished patients cardiovascular risk factors...
Abstract Objectives In this study, we aimed to investigate the effects of long-term administration cardioprotective drug mildronate on concentrations l-carnitine and γ-butyrobetaine in healthy volunteers. Methods Mildronate was administered perorally, at a dosage 500 mg, twice daily. Plasma urine samples were collected weekly. Daily meat consumption within an average, non-vegetarian diet monitored. l-Carnitine, measured using UPLC/MS/MS method. Key findings After 4 weeks, average plasma...
Abstract An ultra‐high‐performance liquid chromatography–mass spectrometry (UPLC/MS/MS) method was developed and validated for the quantification of trimethylamine‐ N ‐oxide (TMAO) simultaneously with TMAO‐related molecules l ‐carnitine γ ‐butyrobetaine (GBB) in human blood plasma. The separation analytes achieved using a Hydrophilic interaction chromatography (HILIC)‐type column ammonium acetate–acetonitrile as mobile phase. TMAO determination according to valid US Food Drug Administration...
Metformin is a widely prescribed antihyperglycemic agent that has been also associated with multiple therapeutic effects in various diseases, including several types of malignancies. There growing evidence regarding the contribution epigenetic mechanisms reaching metformin's goals; however, effect metformin on human cells vivo not comprehensively studied. The aim our study was to examine metformin-induced alterations DNA methylation profiles white blood healthy volunteers, employing...
T helper (Th) 17 cells and interleukin (IL)-17 play a significant role in the pathogenesis of autoimmune thyroid disease (AITD). However, it has recently become clear that Th17 are more heterogeneous exhibit two different phenotypes, whereas IL-23 IL-1β crucial for generation pathogenic lymphocytes. We aimed to investigate association between IL-17 Th17-promoting cytokines AITD by studying immunoexpression patterns IL-17, IL-23, tissue. Following thyroidectomy, 29 patients with (21 cases...
Elevated plasma levels of trimethylamine N-oxide (TMAO) have been proposed as a diet-derived biomarker cardiometabolic disease risk. Caloric restriction is the most common dietary intervention used to improve health; however, novel trends suggest fasting-mimicking diet (FMD) more feasible alternative. FMD variation intermittent fasting, based on caloric and limitation protein sources animal origin, applied in daily cycles during 5-day period. As TMAO intensively produced by gut microbiota...
We treated a patient with type 2 diabetes suffering from chronic venous and neuro-ischemic wounds not healing under standard care local application of autologous bone marrow cells (mBMC) isolated aspirate. This procedure led to reduction wound size, markedly increased vascularization infiltration mononuclear cells, 7 days after treatment, without any signs systemic reaction treatment. Locally applied mBMC could, therefore, provide treatment option in end stage diabetic disorders.
Exercise training induces adaptations in muscle and other tissue mitochondrial metabolism, dynamics, oxidative phosphorylation capacity. Mitochondrial fatty acid oxidation was shown to be pivotal for the anti-inflammatory status of immune cells. We hypothesize that exercise can exert effects influence metabolism peripheral blood mononuclear cells (PBMCs). The aim investigate effect on oxidation-dependent respiration PBMCs. Twelve fasted or fed volunteers first performed incremental-load...
Abstract Background Type 2 diabetes complications cause a serious emotional and economical burden to patients healthcare systems globally. Management of both acute chronic diabetes, which dramatically impair the quality patients' life, is still an unsolved issue in care, suggesting need for early identification individuals with high risk developing complications. Methods We performed genome-wide association study 601 type after stratifying them according presence or absence four types...
Objective(s) High variability in clinical response to metformin is often observed type 2 diabetes (T2D) patients, and it highlights the need for identification of genetic components affecting efficiency therapy. Aim this observational study evaluate role tagSNPs (tagging single nucleotide polymorphisms) from genomic regions coding six transporter genes with respect short-term efficiency. Design 102 6 transporters were genotyped group T2D patients treated 3 months. Methods Most significant...
Metformin, a biguanide agent, is the first-line treatment for type 2 diabetes mellitus due to its glucose-lowering effect. Despite wide application in of multiple health conditions, glycemic response metformin highly variable, emphasizing need reliable biomarkers. We chose RNA-Seq-based comparative transcriptomics approach evaluate systemic effect and highlight potential predictive biomarkers drug-naïve volunteers with vivo. The longitudinal blood-derived transcriptome analysis revealed...
Abstract Objective Low iodine intake during pregnancy may cause thyroid dysfunction, which results in inadequate fetal brain development. In the absence of a universal salt iodization programme, we conducted nationwide survey deficiency pregnant women Latvia. Design A countrywide twenty-cluster survey, with at least twenty per cluster. Participants completed questionnaire on dietary habits concerning ( n 739). Thyroid function (thyroid-stimulating hormone, free thyroxine and thyroperoxidase...
Metformin is a commonly used antihyperglycaemic agent for the treatment of type 2 diabetes mellitus. Nevertheless, exact mechanisms action, underlying various therapeutic effects metformin, remain elusive. The goal this study was to evaluate alterations in longitudinal whole-blood transcriptome profiles healthy individuals after one-week metformin intervention order identify novel molecular targets and further prompt discovery predictive biomarkers response. Next generation sequencing-based...
The glyoxalase enzymes catalyse the conversion of reactive glucose metabolites into non-toxic products as a part cellular defence system against glycation. This study investigated changes in 1 and 2 activities development diabetic complications experimental animal models obesity (Zucker fa/fa rats) type diabetes mellitus (Goto-Kakizaki rats). In contrast to Zucker rats, Goto-Kakizaki rats brain, spinal cord sciatic nerve tissues were significantly reduced by 10, 32 36 %, respectively. Lower...
Context: Selenium supplementation has been suggested for Hashimoto thyroiditis and Graves' ophthalmopathy. Objective, Design: Our aim is to measure selenium status (p-Se, p-SePP), urine iodine (UI) levels iodine/creatinine ratio (UI/C) in different thyroid diseases (n = 416) from four European countries compare the results between patients with without autoimmunity. Results: p-Se p-SePP showed positive correlation did not correlate UI/C. Also, these measurements were higher Italy comparison...