A5073940959- Gut microbiota and health
- Metabolism, Diabetes, and Cancer
- Clostridium difficile and Clostridium perfringens research
- Epigenetics and DNA Methylation
- Genetic Associations and Epidemiology
- Bioinformatics and Genomic Networks
- Diet and metabolism studies
- Diabetes and associated disorders
- Mycobacterium research and diagnosis
- Tryptophan and brain disorders
- Liver Disease Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- COVID-19 and healthcare impacts
- Colorectal Cancer Screening and Detection
- Genomics and Rare Diseases
- Diabetes Treatment and Management
- Pancreatic function and diabetes
- Long-Term Effects of COVID-19
- Nutrition and Health in Aging
- Genetics and Neurodevelopmental Disorders
- Genetics, Bioinformatics, and Biomedical Research
- Genetic Syndromes and Imprinting
- Digestive system and related health
- Helicobacter pylori-related gastroenterology studies
- Advances in Oncology and Radiotherapy
Latvian Biomedical Research and Study Centre
2015-2025
University of Latvia
2018-2022
Riga Stradiņš University
2022
Unidad de Cirugía Artroscópica
2018
Foundation for Ichthyosis and Related Skin Types
2018
Latvia University of Life Sciences and Technologies
2018
Background Metformin is a widely used first-line drug for treatment of type 2 diabetes. Despite its advantages, metformin has variable therapeutic effects, contraindications, and side effects. Here, the very first time, we investigate short-term effect on composition healthy human gut microbiota. Methods We an exploratory longitudinal study design in which sample from individual was control further samples. Eighteen individuals were treated with (2 × 850 mg) 7 days. Stool samples collected...
The human microbiome has emerged as a central research topic in biology and biomedicine. Current studies generate high-throughput omics data across different body sites, populations, life stages. Many of the challenges are similar to other studies, quantitative analyses need address heterogeneity data, specific statistical properties, remarkable variation composition individuals sites. This led broad spectrum machine learning that range from study design, processing, standardization...
The gut microbiome plays a pivotal role in the modulation of host responses during viral infections, and recent studies have underscored its significance context coronavirus disease 2019 (COVID-19). We aimed to investigate dynamics compositional changes COVID-19 patients, addressing both acute phase recovery process, with particular focus on emergence post-COVID-19 conditions. Involving 146 patients 110 healthy controls, this study employed shotgun metagenomics approach for cross-sectional...
Effects of metformin, the first-line drug for type 2 diabetes therapy, on gut microbiome composition in have been described various studies both human subjects and animals. However, details molecular mechanisms metformin action not fully understood. Moreover, there is a significant lack information how affects female mouse models, depending sex metabolic status well controlled experimental setting. Our study aimed to examine metformin-induced alterations diversity, composition, functional...
Blood-based epigenetic markers differentiate metformin responsiveness and tolerance in patients recently diagnosed with type 2 diabetes.
The study was conducted to investigate the effects of metformin treatment on human gut microbiome's taxonomic and functional profile in Latvian population, evaluate correlation these changes with therapeutic efficacy tolerance.In this longitudinal observational study, stool samples for shotgun metagenomic sequencing-based analysis were collected two cohorts. first cohort included 35 healthy nondiabetic individuals (metformin dose 2x850mg/day) at three time-points during administration....
Abstract Background There has been much interest in the possible benefits of microbiome-modulating interventions, such as probiotics, prebiotics, antibiotics ulcerative colitis. Microbiota plays an important role host health and is perturbed by several factors including diseases like coronavirus disease 2019 (COVID-19). Methods From June 2021 to 2022 79 colitis (UC) outpatients from Riga East Clinical University Hospital 78 healthy controls were included a Cross-sectional study. All patients...
Abstract Background Tobacco smoking and alcohol consumption are leading causes of the global burden disease major contributors to premature mortality. The risk factors like cigarette smoking, diet preferences have been identified responsible for ulcerative colitis patient’s microbiome alteration. Methods In 12-month time (June 2021 June 2021) 79 (UC) outpatients from Riga East Clinical University Hospital 78 healthy controls were included in a Cross-sectional study. All patients divided into...
Abstract Background Psychological and physical stress can worsen symptoms contribute to flare-ups in ulcerative colitis (UC) patients. The education level, occupation income level may affect patients during stressful periods such as COVID-19 outbreak. Methods In 12 months time (June 2021 June 2022) 79 outpatients from Riga East Clinical University Hospital 78 healthy controls were included a Cross-sectional study. All divided into groups according medically proven data about status...
Background and Objectives: The human gut microbiome is essential for the health of host affected by antibiotics coronavirus disease 2019 (COVID-19). recognized as a contributing factor in development ulcerative colitis. Specific vitamins probiotics have been demonstrated to positively influence enhancing prevalence expected beneficial microorganisms. Materials Methods: Forty-nine colitis (UC) outpatients from Riga East Clinical University Hospital were enrolled this cross-sectional study...
Metformin is a widely prescribed antihyperglycemic agent that has been also associated with multiple therapeutic effects in various diseases, including several types of malignancies. There growing evidence regarding the contribution epigenetic mechanisms reaching metformin's goals; however, effect metformin on human cells vivo not comprehensively studied. The aim our study was to examine metformin-induced alterations DNA methylation profiles white blood healthy volunteers, employing...
Numerous type 2 diabetes (T2D) polygenic risk scores (PGSs) have been developed to predict individuals’ predisposition the disease. An independent assessment and verification of best-performing PGS are warranted allow for a rapid application models. To date, only 3% T2D PGSs evaluated. In this study, we assessed all (n = 102) presently published in an cohort 3718 individuals, which has not included construction or fine-tuning any so far. We further chose PGS, its performance across major...
Metformin is widely used for treating type 2 diabetes mellitus (T2D). However, the efficacy of metformin monotherapy highly variable within human population. Understanding potential indirect or synergistic effects on gut microbiota composition and encoded functions could potentially offer new insights into predicting treatment designing more personalized treatments in future. We combined targeted metabolomics metagenomic profiling microbiomes newly diagnosed T2D patients before after therapy...
Abstract Background Type 2 diabetes complications cause a serious emotional and economical burden to patients healthcare systems globally. Management of both acute chronic diabetes, which dramatically impair the quality patients' life, is still an unsolved issue in care, suggesting need for early identification individuals with high risk developing complications. Methods We performed genome-wide association study 601 type after stratifying them according presence or absence four types...
Metformin, a biguanide agent, is the first-line treatment for type 2 diabetes mellitus due to its glucose-lowering effect. Despite wide application in of multiple health conditions, glycemic response metformin highly variable, emphasizing need reliable biomarkers. We chose RNA-Seq-based comparative transcriptomics approach evaluate systemic effect and highlight potential predictive biomarkers drug-naïve volunteers with vivo. The longitudinal blood-derived transcriptome analysis revealed...
Introduction Research findings of the past decade have highlighted gut as main site action oral antihyperglycemic agent metformin despite its pharmacological role in liver. Extensive evidence supports metformin’s modulatory effect on composition and function microbiota, nevertheless, underlying mechanisms host responses remain elusive. Our study aimed to evaluate metformin-induced alterations intestinal transcriptome profiles at different metabolic states. Methods The high-fat diet-induced...
Familial hypercholesterolemia (FH) is one of the commonest monogenic disorders, predominantly inherited as an autosomal dominant trait. When untreated, it results in early coronary heart disease. The vast majority FH remains undiagnosed Latvia. identification and treatment affected individuals remain a challenge worldwide. Most cases are caused by mutations four genes, APOB, LDLR, PCSK9, or LDLRAP1. spectrum disease-causing variants very diverse variation detection panels usually used its...
Metformin is a commonly used antihyperglycaemic agent for the treatment of type 2 diabetes mellitus. Nevertheless, exact mechanisms action, underlying various therapeutic effects metformin, remain elusive. The goal this study was to evaluate alterations in longitudinal whole-blood transcriptome profiles healthy individuals after one-week metformin intervention order identify novel molecular targets and further prompt discovery predictive biomarkers response. Next generation sequencing-based...
Introduction. Although the presence of micro-organisms in blood healthy humans is a relatively new concept, there growing amount evidence that might have its own microbiome.Gap Statement. Previous research has targeted taxonomic composition microbiome using DNA-based sequencing methods, while little information known about microbial transcripts obtained from and their relation to conditions connected with increased gut permeability.Aim. To detect potentially alive active investigate...
ABSTRACT The human gut teems with a diverse ecosystem of microbes, yet non-bacterial portions that community are overlooked in studies metabolic diseases firmly linked to bacteria. Type 2 diabetes mellitus (T2D) is associated compositional shifts the bacterial microbiome and mycobiome, fungal portion microbiome. However, whether T2D and/or metformin treatment underpins changes unresolved. To differentiate these effects, we curated mycobiome cohort spanning 1,000 samples across five countries...
Background and Objectives: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2 is the new that caused disease 2019 (COVID-19) outbreak. Studies have increasingly reported involvement of organs outside system, including gastrointestinal tract. Data on association between COVID-19 ulcerative colitis (UC) are lacking. Materials Methods: In this one-centre cross-sectional study, 49 patients with UC from Riga East Clinical University Hospital outpatient clinic were included June 2021 to...
Abstract Background The microbiome contributes to the pathogenesis of ulcerative colitis (UC) but contribution different lifestyle and environmental factors compositional variability gut microbiota is unclear. Also, it known that plays an important role in infectious diseases like coronavirus disease 2019 (COVID-19). Methods In 6-month time (June 2021 December 2021) 49 UC outpatients from Riga East Clinical University Hospital were included a Cross-sectional study. All patients divided into...
Abstract Maturity-onset Diabetes of the Young (MODY) presents a diagnostic challenge, with large proportion cases lacking identifiable genetic mutations. This study investigates basis MODY in Latvian cohort comprising 66 suspected families, contrasted 177 non-diabetic controls. Employing panel-based and whole-genome sequencing (WGS), we identified 22 pathogenic mutations three genes ( GCK , HNF1A HNF4A ), eight them being novel. We selected tested best-performing population specific type 1...