A5037011704- Advanced Biosensing Techniques and Applications
- Gene expression and cancer classification
- Salivary Gland Disorders and Functions
- Genomics and Chromatin Dynamics
- Systemic Lupus Erythematosus Research
- Cancer Cells and Metastasis
- RNA Research and Splicing
- Monoclonal and Polyclonal Antibodies Research
- DNA Repair Mechanisms
- Inflammatory Myopathies and Dermatomyositis
- Cell Image Analysis Techniques
- Salivary Gland Tumors Diagnosis and Treatment
- Immunodeficiency and Autoimmune Disorders
- Lymphoma Diagnosis and Treatment
- Alzheimer's disease research and treatments
- Ocular Surface and Contact Lens
- Dementia and Cognitive Impairment Research
- Urticaria and Related Conditions
- Immunotherapy and Immune Responses
- Cancer-related Molecular Pathways
- Systemic Sclerosis and Related Diseases
- interferon and immune responses
- Nuclear Structure and Function
- Eosinophilic Disorders and Syndromes
- Celiac Disease Research and Management
Quanterix (United States)
2022-2025
Quantix (United States)
2024
IMMCO Diagnostics (United States)
2009-2021
Nova Medical (United States)
2019-2021
University at Buffalo, State University of New York
2005-2014
New York University
2005-2006
State University of New York
2005
BACKGROUND: Phosphorylated tau (p-tau) is a specific blood biomarker for Alzheimers disease (AD) pathology. Multiple p-tau biomarkers on several analytical platforms are poised clinical use. The Association Global Biomarker Standardisation Consortium plasma phospho-tau Round Robin study engaged assay developers in blinded case-control p-tau, aiming to learn which assays provide the largest fold-changes AD compared non-AD, have strongest relationship between and cerebrospinal fluid (CSF),...
Abstract Matrin 3 (matr3), an abundant protein of the internal nuclear matrix, has been linked to a variety functional events. As step toward defining its multifunctional nature, we have studied association matr3 with chromosome territories and identified potential interacting proteins. A similar staining pattern was observed in fixed WI38 fibroblast cells live HeLa using matr3‐GFP construct. Matr3 detected throughout autosomal active X territories. Conversely, strikingly excluded from...
Sjogren's syndrome (SS) is a chronic autoimmune disease mainly affecting salivary and lacrimal glands. Current diagnostic criteria for SS utilize anti-Ro anti-La as serological markers. Animal models have identified novel autoantibodies, anti-salivary gland protein 1 (SP1), anti-carbonic anhydrase 6 (CA6) parotid secretory (PSP). These antibodies are seen in the animals at an earlier stage of than anti-La. The current studies were designed to evaluate these autoantibodies sera...
Abstract Background Evidence in imaging studies suggests that there may be differences glandular involvement Sjogren’s syndrome (SS) depending on the stage of disease. No detailed histological are available to show if any such difference at various time periods and stages SS. This cross sectional study examines inflammatory changes mouse models SS ages. Methods The major salivary lacrimal glands were studied ages 3, 6, 9, 12, 15 18 months both sexes well characterized SS, non-obese diabetes...
The Alzheimer's Association Global Biomarker Standardization Consortium conducted a blinded case-control study to learn which phosphorylated tau (p-tau) assays provide the largest fold-changes in disease (AD) versus non-AD and show commutability measuring patient samples candidate certified reference materials (CRMs). Thirty-three different p-tau measured paired plasma cerebrospinal fluid (CSF) from 40 participants (25 with "AD pathology" 15 "non-AD by CSF amyloid beta [Aβ]42/Aβ40 p-tau181...
Activation of NMDA receptors leads to activation cAMP-dependent protein kinase (PKA). The main substrates phosphorylated by PKA following receptor remain unidentified. aim this work was identify a major substrate in cerebellar neurones culture, and assess whether phosphorylation may be involved neuronal death induced excessive activation. identified MALDI-TOFF fingerprinting as the nuclear protein, matrin 3. PKA-mediated 3 is followed its degradation. rat brain vivo ammonia injection also...
Abstract Higher order chromatin organization in concert with epigenetic regulation is a key process that determines gene expression at the global level. The of dynamic domains and their associated protein factors intertwined nuclear function to create higher levels functional zones within cell nucleus. As step towards elucidating dynamics these zones, we have investigated spatial proximities among constellation functionally related sites are found euchromatic regions nucleus including: HP1γ,...
Abstract Primary Sjögren’s syndrome (pSS) is a complex autoimmune disease starting in the salivary and lacrimal glands continuing to involve lungs kidneys with eventual development of lymphoma. Many studies have emphasized role type 1 IFN (IFN-α) lymphotoxin α (LTα) pathogenesis disease. The present were designed delineate IFN-α pSS using an animal model, IL-14α (IL14αTG) transgenic mouse. IL14αTG mice lacking IFNR (IL14αTG.IFNR−/−) had same submandibular gland injury as did mice, but they...
We used a combination of spectral karyotyping, array comparative genomic hybridization, and cDNA microarrays to gain insights into the structural functional changes genome in MCF10 human breast cancer progression model cell lines. Spectral karyotyping data showed several chromosomal aberrations hybridization analysis identified numerous gains losses that might be involved toward cancer. Analysis expression levels genes located within these regions revealed lack correlation between...
Indirect immunofluorescence (IIF) using human epithelial cell (HEp-2) substrate is a widely used and the recommended method for screening of antinuclear antibodies (ANA). Dense fine speckled (DFS70) pattern on HEp-2 has been reported in various healthy disease groups. Interpretation DFS70 can be challenging conventional due to its similarity some associated patterns. The high prevalence autoantibodies normal population, lack association with particular group general negative systemic ANA...
Abstract Genomic DNA in mammalian cells is organized into ∼1 Mbp chromatin domains (ChrD) which represent the basic structural units for compaction, replication, and transcription. Remarkably, ChrD are highly dynamic undergo both translational movement configurational changes. In this study, we introduce an automated motion tracking analysis to measure, 2D 3D, linear displacement of early, mid late S‐phase replicated over short time periods (<1 sec). We conclude that previously identified...
Background: Antibodies to salivary gland protein 1 (SP1), carbonic anhydrase 6 (CA6) and parotid secretory (PSP) were discovered in an animal model of Sjogren’s syndrome (SS). Their expression was noted patients with SS, especially those lower focus scores on lip biopsies early disease lacking antibodies Ro La. Objective: The current studies evaluated these autoantibodies long-standing SS expressing high levels anti-Ro secondary systemic lupus erythematosus (SLE), sclerosis (SSc) mixed...
Dry eye is a common problem in Ophthalmology and may occur for many reasons including Sjogren's syndrome (SS). Recent studies have identified autoantibodies, anti-salivary gland protein 1 (SP1), anti-carbonic anhydrase 6 (CA6) anti-parotid secretory (PSP), which early the course of SS. The current were designed to evaluate how patients with idiopathic dry no evidence systemic diseases from practice these autoantibodies. Patients clinic normal controls assessed by Schirmer's test tear flow....
(1) Background: Myositis specific antibodies (MSA) represent important diagnostic and stratification tools in idiopathic inflammatory myositis (IIM) patients. Here we aimed to evaluate the clinical performance of MSA profiled by a novel particle based multi-analyte technology (PMAT) IIM subsets thereof. (2) Methods: 264 patients 200 controls were tested for using PMAT (Inova Diagnostics, research use only). Diagnostic was analyzed composite scores generated. (3) Results: The...
<b><i>Purpose:</i></b> The purpose of this report is to describe 2 patients with persistent severe dry eyes, positive Schirmer tests for Sjogren's syndrome (SS) but lacking antibodies either Ro or La. These were diagnosed have SS by detecting salivary gland protein 1 (Sp1) and parotid secretory (PSP). This emphasizes the existence who lack La may therefore be misdiagnosed. Detection novel autoantibodies, including Sp1 PSP, are helpful in identifying these patients....
To compare frequencies of autoreactive antibody responses to endogenous disease-associated antigens in healthy controls (HC), relapsing and progressive MS assess their associations with clinical MRI measures disease progression. Methods The study analyzed 969 serum samples from 315 HC, 411 remitting (RR-MS), 128 secondary (SP-MS), 33 primary (PP-MS) 82 patients other neurological diseases for autoantibodies against two putative CSF114(Glc) KIR4.1a KIR4.1b 24 key linked such as vasculitis,...