A5034542622- Insect Resistance and Genetics
- Genetically Modified Organisms Research
- Pesticide Exposure and Toxicity
- Pesticide Residue Analysis and Safety
- Pesticide and Herbicide Environmental Studies
- Insect and Pesticide Research
- Environmental Toxicology and Ecotoxicology
- Toxic Organic Pollutants Impact
- Transgenic Plants and Applications
- Biosensors and Analytical Detection
- Biosimilars and Bioanalytical Methods
- Effects and risks of endocrine disrupting chemicals
- Carcinogens and Genotoxicity Assessment
- Viral Infectious Diseases and Gene Expression in Insects
- Legume Nitrogen Fixing Symbiosis
- Plant tissue culture and regeneration
- CRISPR and Genetic Engineering
- Weed Control and Herbicide Applications
- Analytical Chemistry and Chromatography
- Electrowetting and Microfluidic Technologies
- Quantum Dots Synthesis And Properties
- Innovative Microfluidic and Catalytic Techniques Innovation
- Computational Drug Discovery Methods
- Animal Genetics and Reproduction
- Advanced Biosensing Techniques and Applications
Corteva (United States)
2018-2022
Iowa State University
2005
University of Maine
2005
University of California, Davis
1999-2004
Cancer Research Center
2001-2004
Lawrence Livermore National Laboratory
2000
University of Nevada, Las Vegas
1999
Louisiana State University Agricultural Center
1997
Carboxylesterases hydrolyze numerous endogenous and exogenous ester-containing compounds. They play a role in the detoxification of many agrochemicals including pyrethroids, organophosphates, carbamates. Research on these enzymes is still developing there are several topics that should be addressed to further investigations this area. This paper focuses number issues enzyme nomenclature, catalytic mechanism, substrate specificity, agrochemical metabolism, insecticide resistance environmental...
Engineered glyphosate resistance is the most widely adopted genetically modified trait in agriculture, gaining widespread acceptance by providing a simple robust weed control system. However, extensive and sustained use of as sole mechanism has led to field selection for glyphosate-resistant weeds induced significant population shifts with inherent tolerance glyphosate. Additional mechanisms that can complement crops are, therefore, urgently needed. 2,4-dichlorophenoxyacetic acid (2,4-D) an...
A method for simply and cheaply preparing inorganic phosphor nanoparticles of Eu2O3 as labels in biology has been demonstrated with a simple microwave-assisted surface chemistry. The capping process adds silane layer to the particles provides amine groups that can be used biological conjugation. also protects during conjugation retain their desirable optical properties are typical europium, is, spectrally narrow, red emission long fluorescence lifetime. application nanoparticle an...
Immunoassays for biotechnology engineered proteins are used by AgBiotech companies at numerous points in product development and feed food suppliers compliance contractual purposes. Although use the technology during seed production, other stakeholders from supply chains, such as commodity, food, companies, well third-party diagnostic testing also rely on immunoassays a number of The primary is to verify presence or absence genetically modified (GM) material quantify amount GM present...
Permethrin is a predominant pyrethroid widely used in agriculture and public health. A competitive enzyme-linked immunosorbent assay (ELISA) for the detection of permethrin was developed. Two haptens, trans- cis-isomers 3-(4-aminophenoxy)benzyl-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate, were synthesized conjugated with thyroglobulin as immunogens. Four antisera generated screened against six different coating antigens. The resulting ELISA has an I50 value 2.50 μg/L...
The pyrethroids are one of the most heavily used insecticide classes in world. It is important to develop sensitive and rapid analytical techniques for environmental monitoring assessment human exposure these compounds. Because major contain a phenoxybenzyl group phenoxybenzoic acid (PBA) common metabolite form or intermediate, PBA might be as biomarker pyrethroids. A selective immunoassay pyrethroid was developed. Rabbits were immunized with 3-[4-(3-carboxyphenoxy)phenoxy] N-thyroglobulin...
A competitive enzyme-linked immunosorbent assay was developed for the detection of pyrethroid insecticide esfenvalerate. Two haptens containing amine or propanoic acid groups on terminal aromatic ring fenvalerate molecule were synthesized and coupled to carrier proteins as immunogens. Five antisera produced screened against eight different coating antigens. The that had least interference most sensitive esfenvalerate optimized characterized. I50 30 ± 6.2 μg/L, lower limit (LDL) 3.0 1.8 μg/L....
Abstract Recent agrochemical usage patterns suggest that the use of organophosphate (OP) pesticides will decrease, resulting in a concomitant increase pyrethroid usage. Pyrethroids are known for their potential toxicity to aquatic invertebrates and many fish species. Current identification evaluation (TIE) techniques able detect OPs, but have not been optimized pyrethroids. Organophosphate methods depend upon piperonyl butoxide (PBO) identify OP-induced toxicity. However, PBO TIE assays be...
A highly selective enzyme-linked immunosorbent assay (ELISA) has been developed for the quantitative detection of Cry1Ac protein expressed in transgenic cotton. Two Cry1Ac-specific monoclonal antibodies (MAb), Kbt and 158E6, were selected to form a sandwich format ELISA. The MAb was used as capture antibody, 158E6 conjugated with horseradish peroxidase served antibody. optimized validated different cotton matrices. Tissues extracted phosphate-buffered saline containing 0.05% Tween 20 1%...
The practice of immunoassay has experienced a widespread transition from radioisotopic labeling to nonisotopic over the last two decades. Radioisotope labels have drawbacks that hamper their applications: (i) perceived radiation hazards reagents, (ii) regulatory requirements and disposal problems working with radioactive materials, (iii) short shelf-life labeled reagents. advantage isotopic is incorporation into analytes without altering structure or reactivity, as often case ELISA...
A competitive enzyme-linked immunosorbent assay (ELISA) for the detection of deltamethrin was developed. Two haptens, cyano[3-(4-aminophenoxy)phenyl]methyl 1R-cis-3-(2,2−dibromoethenyl)-2,2-dimethylcyclopropanecarboxylate and 3-[(±)-cyano[1R-cis-3-(2,2−dibromoethenyl)-2,2-dimethylcyclopropan ecarbonyloxy]methyl]phenoxyacetic acid, were synthesized conjugated with thyroglobulin as immunogens. Four antisera generated screened against six different coating antigens. The that most sensitive...
A competitive enzyme-linked immunosorbent assay (ELISA) for the detection of cypermethrin was developed. Two haptens, trans- and cis-isomers 3-[(±)-cyano-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarbonyloxy]methyl]phenoxyacetic acid, were conjugated with thyroglobulin as immunogens. Four antisera generated screened against six different coating antigens. The that most sensitive optimized characterized. IC50 13.5 ± 4.3 μg/L, lower limit (LDL) 1.3 0.5 μg/L. This ELISA had relatively low...
To evaluate the potential of Bacillus thuringiensis (Bt) Cry1F protein accumulation in soil, transgenic corn containing event DAS-01507-1 encoding cry1F gene was grown three field sites for 3 consecutive yr, and plants were incorporated into soil through postseason tillage or no each year. Soil samples collected from these fields, level determined using an enzyme-linked immunosorbent assay (ELISA) with a synthetic invertebrate gut fluid as extraction buffer. The ELISA validated matrices over...
Since the commercial introduction of genetically modified (GM) plants in agriculture over two decades ago, technology developers and regulatory authorities have gained significant experience assessing their safety based on potential impact to humans, animals environment. Over 3500 independent agency reviews positively concluded GM for food feed. Yet, divergent increased requirements led delayed asynchronous approvals, restricted access innovative products farmers consumers. With accumulated...
The pyrethroids are one of the most heavily used insecticide classes in world. Sensitive and rapid analytical techniques needed for assessments human exposure to these compounds. Highly sensitive selective ELISAs glycine conjugates esfenvalerate key metabolites phenoxybenzoic acid (PBA) s-fenvalerate (sFA) were developed. Rabbits immunized with either N-(3-phenoxybenzoyl)-4-amino-l-phenylalanine−fetuin or N-[(S)-4-chloro-2-(methylethyl)benzeneacetyl]-4-amino-l-phenylalanine−fetuin, all sera...
As part of the regulatory approval process in Europe, comparison endogenous soybean allergen levels between genetically engineered (GE) and non-GE plants has been requested. A quantitative multiplex analytical method using tandem mass spectrometry was developed validated to measure 10 potential allergens from seed. The implemented at six laboratories demonstrate robustness further applied three field studies across multiple growing seasons (including 21 varieties) assess natural variation...
Quantitative monitoring of Bacillus thuringiensis (Bt) insecticidal crystal proteins in soil has been hampered by the lack efficient extraction/detection methods. A novel approach for simple and effective Bt protein extraction was explored evaluating solutions from invertebrate gut fluids. Marine worm fluids were identified as promising extracting soil. An artificial fluid based on these marine developed using commercially available chemicals evaluated its ability to extract On basis...
Currently, traditional immunochemistry technologies such as enzyme-linked immunosorbent assays (ELISA) are the predominant analytical tool used to measure levels of recombinant proteins expressed in genetically engineered (GE) plants. Recent advances agricultural biotechnology have created a need develop methods capable selectively detecting and quantifying multiple complex matrices because increasing numbers transgenic being coexpressed or "stacked" achieve tolerance herbicides provide...