A5100361095- Protein Hydrolysis and Bioactive Peptides
- Enzyme Production and Characterization
- Seaweed-derived Bioactive Compounds
- Meat and Animal Product Quality
- Phytase and its Applications
- Biochemical effects in animals
- Aquaculture Nutrition and Growth
- Biofuel production and bioconversion
- Polyamine Metabolism and Applications
- Hydrogels: synthesis, properties, applications
- Echinoderm biology and ecology
- Nanocomposite Films for Food Packaging
- Marine Bivalve and Aquaculture Studies
- Identification and Quantification in Food
- Viral Infectious Diseases and Gene Expression in Insects
- biodegradable polymer synthesis and properties
- Selenium in Biological Systems
- Insect Utilization and Effects
- Microbial metabolism and enzyme function
- Protein purification and stability
- Probiotics and Fermented Foods
- Microbial Metabolic Engineering and Bioproduction
- Proteoglycans and glycosaminoglycans research
- Food Industry and Aquatic Biology
- Legume Nitrogen Fixing Symbiosis
Sichuan University of Science and Engineering
2012-2024
Ocean University of China
2017-2019
Nanjing Agricultural University
2017
National Quarantine Station
2014
Guangxi Medicinal Botanical Garden
2011
Zhejiang Ocean University
2011
Southwest University
2010
South China University of Technology
2007
Alginate lyases play an important role in preparation of alginate oligosaccharides. Although a large number have been characterized, reports on directional oligosaccharides by are still rather less. Here, gene alyM encoding new lyase AlyM was cloned from Microbulbifer sp. Q7 and expressed Escherichia coli. exhibited the maximumactivity at pH 7.0 55°C showed special preference to poly-guluronic acid (polyG). Glycine promoted extracellular secretion 3.6 times. PBS glycerol significantly...
Alginate lyase degrades alginate by the β-elimination mechanism to produce oligosaccharides with special bioactivities. The low thermal stability of limits its industrial application. In this study, introducing disulfide bonds while using rational design methodology enhanced cAlyM from Microbulbifer sp. Q7. Enzyme catalytic sites, secondary structure, spatial configuration, and molecular dynamic simulation were comprehensively analyzed. When compared cAlyM, mutants D102C-A300C G103C-T113C...
Abstract Rapeseed meal (RSM) is an important source of protein, but its value limited due to the poor digestibility and presence many antinutritional factors. In this study, a two‐step biological method was developed for detoxifying RSM increasing protein value. first stage, various enzymes proteases were produced by Aspergillus niger during solid‐state fermentation (SSF). second coordinated enzymatic hydrolysis employed further degrade factors macromolecular proteins in fermented RSM....
Abstract Production of chitosan and its derivatives by traditional methods involves the excessive use a reaction solution comprised sodium hydroxide hydrochloric acid. Waste water resulting from this process has limited application as fertilizer causes serious environmental pollution. Specifically, waste contains high levels dissolved nitrogen minerals shrimp shells. In study, an eco-friendly method was established to produce chitooligosaccharides (COS) with different degrees deacetylation...
κ-Carrageenase belongs to glycoside hydrolase family 16 and cleaves the β-(1→4) linkages of κ-carrageenan. In this study, genes encoding full-length (cgkZ), Por secretion tail-truncated (cgkZΔPst) carbohydrate binding domain-truncated (cgkZΔCBM) κ-carrageenase proteins were expressed in Pichia pastoris. The copy numbers gene cgkZ, cgkZΔPst cgkZΔCBM 7, 7 6, respectively. enzymatic activities recombinant enzymes reached 4.68, 5.70, 3.02 U/mL, respectively, after 120 h shake flask fermentation...
Chondroitinase (ChSase), a type of glycosaminoglycan (GAG) lyase, can degrade chondroitin sulfate (CS) to unsaturate oligosaccharides, with various functional activities. In this study, ChSase AC II from newly isolated marine bacterium Arthrobacter sp. CS01 was cloned, expressed in Pichia pastoris X33, purified, and characterized. II, molecular weight approximately 100 kDa specific activity 18.7 U/mg, showed the highest at 37 °C pH 6.5 maintained stability broad range (5⁻7.5) temperature...
In this study, with the aid of a constitutive promoter, and co-expression chaperone transcription factor (TF) genes, expression enzymatic activity recombinant κ-carrageenase in Pichia pastoris containing truncated gene cgkZΔPst (GS115/pPIC9K-cgkZΔPst) was enhanced. The P. strain glyceraldehyde-3-phosphate dehydrogenase (GAP) promoter enabled without methanol induction, 2.73 U/mL after 96 h shake flask fermentation at 22 °C. increased to 7.96 under induction during growth, showing 1.4-fold...
This study was designed to characterize in vitro digested products of proteins from four commercial meat products, including dry-cured ham, cooked emulsion-type sausage and sausage. The samples were homogenized incubated with pepsin trypsin. digestibility particle sizes measured. Nano LC-MS/MS applied peptides. results showed the highest lowest size ham (P<0.05), while opposite for (P<0.05). analysis revealed that had greatest number 750-3500 Da Mw peptides pepsin-digested products. In...
Anchovy (Engraulis japonicus), a small, low-valued, marine fish, and soybean meal (Glycine max), by-product of oil extraction, are both abundant protein resources (approximately 65 46 g per 100 dry weight, respectively). In this study, water-soluble powder from anchovy was obtained by two processes, endogenous enzymatic hydrolysis (stage 1), solid-state fermentation residue 2). The parameters stage were optimized with single factor experiments using central composite design validated....
In order to evaluate the high-value application of squid by-products yielded hydrolysate, process preparation and purification technology, chemical composition in vitro antioxidant activity hydrolysate were investigated. The optimal conditions papain hydrolysis obtained by single-factor experiments orthogonal test with DPPH• scavenging ratio as index, amino acid was analysed automatic analyzer, isolated a Sephadex G-25 column. Based on experiments, being 53.96 % gained under condition...
Brewer’s spent grain (BSG) protein extracted from BSG was hydrolyzed using Alcalase to produce hydrolysate. hydrolysate fractionated by ultrafiltration obtain brown color peptides. Antioxidant activity of peptides analyzeded and compared with reduced glutathione (GSH). exhibited 50% scavenging activities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl superoxide radical concentrations less than 0.8 mg/mL, 0.6 mg/mL respectively. The reducing power 0.70 at the concentration 2.00...