A5089984535- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- biodegradable polymer synthesis and properties
- Polyamine Metabolism and Applications
- Amino Acid Enzymes and Metabolism
- Clostridium difficile and Clostridium perfringens research
- Enzyme Production and Characterization
- Protein Hydrolysis and Bioactive Peptides
- Microbial Metabolites in Food Biotechnology
- Microbial Natural Products and Biosynthesis
- Biochemical Acid Research Studies
- Enzyme Structure and Function
- Cancer Research and Treatments
- Muscle metabolism and nutrition
- Food composition and properties
- Glycosylation and Glycoproteins Research
- Electrochemical sensors and biosensors
- Protein Structure and Dynamics
- Mass Spectrometry Techniques and Applications
- GABA and Rice Research
- Transgenic Plants and Applications
- Diet, Metabolism, and Disease
- Catalysis for Biomass Conversion
- Nuclear and radioactivity studies
University of Iowa
2025
University of Delaware
2024
University of Tennessee at Knoxville
2019-2023
Oak Ridge National Laboratory
2019-2023
Konkuk University
2013-2018
Konkuk University Medical Center
2015
Abstract Itaconate, a C 5 unsaturated dicarboxylic acid, is an important chemical building block that used in manufacturing high-value products, such as latex and superabsorbent polymers. Itaconate produced by fermentation of sugars the filamentous fungus Aspergillus terreus . However, A. involves long period formation various byproducts, resulting high production costs. E. coli has been developed alternative for producing itaconate. glucose gives low conversion yields productivity. Here, we...
Robust and efficient enzymes are essential modules for metabolic engineering synthetic biology strategies across biological systems to engineer whole-cell biocatalysts. By condensing an acyl-CoA alcohol, alcohol acyltransferases (AATs) can serve as interchangeable microbial biosynthesis of a diverse class ester molecules with broad applications flavors, fragrances, solvents, drop-in biofuels. However, the current lack robust AATs significantly limits their compatibility heterologous...
Consolidated bioprocessing (CBP) of lignocellulosic biomass uses cellulolytic microorganisms to enable enzyme production, saccharification, and fermentation produce biofuels, biochemicals, biomaterials in a single step. However, understanding redirecting metabolisms these compatible with CBP are limited. Here, thermophile Clostridium thermocellum was engineered demonstrated be integrated Co-solvent Enhanced Lignocellulosic Fractionation (CELF) pretreatment for conversion hardwood poplar into...
Exopolysaccharide from psychrotrophic Arctic glacier soil bacterium<italic>Flavobacterium</italic>sp. ASB 3-3.
This study aims to increase the 3-hydroxyvalerate (3HV) fraction in poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(HB-co-HV)] using succinyl-CoA synthase.Escherichia coli YH090, a polyhydroxyalkonate (PHA)-producing strain, was further engineered for overexpression of synthase genes (sucCD), and examined P(HB-co-HV) copolymer production presence various precursor molecules mixture analysis. Glycerol, succinate propionate were screened as important factors controlling intracellular PHA...
Esters are versatile chemicals and potential drop-in biofuels. To develop a sustainable production platform, microbial ester biosynthesis using alcohol acetyltransferases (AATs) has been studied for decades. Volatility of esters endows high-temperature fermentation with advantageous downstream product separation. However, due to the limited thermostability AATs known, largely relied on use mesophilic microbes. Therefore, developing thermostable is important directly from lignocellulosic...
Acetic acid is an abundant material that can be used as a carbon source by microorganisms. Despite its abundance, toxicity and low energy content make it hard to utilize sole for biochemical production. To increase acetate utilization isobutanol production with engineered Escherichia coli, the feasibility of utilizing metabolic engineering was investigated. The expression acs, pckA, maeB increased up 26%, addition TCA cycle intermediates indicated enhance For from acetate, uptake rates NADPH...
Medium-chain esters are versatile chemicals with broad applications as flavors, fragrances, solvents, and potential drop-in biofuels. Currently, these largely produced by the conventional chemical process that uses harsh operating conditions requires high energy input. Alternatively, microbial conversion route has recently emerged a promising platform for sustainable renewable ester production. The biosynthesis pathways can utilize either lipases or alcohol acyltransferase (AAT), but...
Short-chain esters are versatile chemicals that can be used as flavors, fragrances, solvents, and fuels. The de novo ester biosynthesis consists of diverging converging pathway submodules, which is challenging to engineer achieve optimal metabolic fluxes selective product synthesis. Compartmentalizing the submodules into specialist cells facilitate modularization labor division a promising solution. Here, we engineered synthetic Escherichia coli coculture with compartmentalized sugar...