A5044768087- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Viral Infectious Diseases and Gene Expression in Insects
- biodegradable polymer synthesis and properties
- Fungal and yeast genetics research
- Protein purification and stability
- Amino Acid Enzymes and Metabolism
- Microplastics and Plastic Pollution
- GABA and Rice Research
- Polyamine Metabolism and Applications
- Aquaculture disease management and microbiota
- Probiotics and Fermented Foods
- Microbial infections and disease research
- Analytical Chemistry and Chromatography
- Biochemical and biochemical processes
- Plant biochemistry and biosynthesis
- Lipid metabolism and biosynthesis
- Microbial Natural Products and Biosynthesis
- Fibroblast Growth Factor Research
- Animal Genetics and Reproduction
- Algal biology and biofuel production
- Enzyme Production and Characterization
- 3D Printing in Biomedical Research
- Microbial Metabolism and Applications
Korea University of Science and Technology
2016-2025
Korea Research Institute of Bioscience and Biotechnology
2016-2025
Cheongju University
2019-2024
Korea Advanced Institute of Science and Technology
2020
Daejeon University
2019
Government of the Republic of Korea
2019
Dankook University
2011-2012
National University of Singapore
2011
Biotechnology Research Center
2009
Korean College of Helicobacter and Upper Gastrointestinal Research
2007
A noninvasive and selective therapy, photodynamic therapy (PDT) is widely researched in clinical fields; however, the lower efficiency of PDT can induce unexpected side effects. Mitochondria are extensively as target sites to maximize effects because they play crucial roles metabolism be used cancer markers due their high transmembrane potential. Here, a mitochondria targeting therapeutic agent (MitDt) developed. This photosensitizer synthesized from heptamethine cyanine dyes, which...
Abstract Background Pichia pastoris has been recognized as an effective host for recombinant protein production. A number of studies have reported improving this expression system. However, its physiology and cellular metabolism still remained largely uncharacterized. Thus, it is highly desirable to establish a systems biotechnological framework, in which comprehensive silico model P. can be employed together with high throughput experimental data analysis, better understanding the...
Recently, the bio-production of α,ω-dicarboxylic acids (DCAs) has gained significant attention, which potentially leads to replacement conventional petroleum-based products. In this regard, lipid accumulating yeast Candida tropicalis, been recognized as a promising microbial host for DCA biosynthesis: it possess unique ω-oxidation pathway where terminal carbon α-fatty is oxidized form DCAs with varying chain lengths. However, despite such industrial importance, its cellular physiology and...
Abstract Background Growth factors (GFs) are signaling proteins that affect cellular processes such as growth, proliferation, and differentiation. GFs used cosmeceuticals, exerting anti-wrinkle, anti-aging, whitening effects, also pharmaceuticals to treat wounds, growth failure, oral mucositis. However, in mammalian bacterial cells, low productivity expression inclusion bodies, respectively, of does not satisfy the consumer demand. Here, we aimed develop a system produces high yields soluble...
Isobutylene, a monomer for butyl rubber production, is traditionally obtained via the thermal cracking of natural gas. However, this route should be superseded by those that rely on chemical or biological valorization renewables to reduce our dependence fossil resources. Despite extensive research biobased rubber, its economically viable large-scale production from bioisobutylene remains underexplored. This study develops process microbial isobutanol and conversion isobutylene rubber. The...
As a biodegradable plastic, polyhydroxybutyrate (PHB) has relatively poor mechanical properties, preventing its wider use. Various plasticizers have been studied to improve the properties of PHB; however, due slow degradation speed in soil environment and lack evaluation methods, studies on PHB with are rarely reported. In this study, by applying Microbulbifer sp. SOL66, which is able degrade very quickly, benign plasticizer was evaluated good degradability, not inhibiting microbial...
As a sustainable industrial process, the production of dicarboxylic acids (DCAs), used as precursors polyamides, polyesters, perfumes, plasticizers, lubricants, and adhesives, from vegetable oil has continuously garnered interest. Although yeast Candida tropicalis been host for DCA production, additional strains are continually investigated to meet productivity thresholds needs. In this regard, Wickerhamiella sorbophila, potential candidate strain, screened. However, lack genetic...
To develop a functional phosphate-regulated promoter in Pichia pastoris, phosphate-responsive gene, PHO89, which encodes putative sodium (Na(+))-coupled phosphate symporter, was isolated. Sequencing analyses revealed 1,731-bp open reading frame encoding 576-amino-acid polypeptide with 12 transmembrane domains. The properties of the PHO89 (P(PHO89)) were investigated using bacterial lipase gene as reporter 5-liter jar fermentation experiments. P(PHO89) tightly regulated by and highly...
We have developed a gamma-aminobutyric acid (GABA) production technique using his-tag mediated immobilization of Escherichia coli-derived glutamate decarboxylase (GAD), an enzyme that catalyzes the conversion to GABA. The GAD was obtained at 1.43 g/L from GAD-overexpressed E. coli fermentation and consisted 59.7% monomer, 29.2% dimer 2.3% tetramer with 97.6% soluble form total GAD. harvested immobilized metal affinity gel yield 92%. Based on investigation specific activity reaction...
Acetic acid is an abundant material that can be used as a carbon source by microorganisms. Despite its abundance, toxicity and low energy content make it hard to utilize sole for biochemical production. To increase acetate utilization isobutanol production with engineered Escherichia coli, the feasibility of utilizing metabolic engineering was investigated. The expression acs, pckA, maeB increased up 26%, addition TCA cycle intermediates indicated enhance For from acetate, uptake rates NADPH...
Sebacic acid is an aliphatic ten-carbon dicarboxylic (1,10-decanedioic acid) with a variety of industrial applications. Here, we present its microbial production, purification, and polymerization.