A5028073910- Plant Reproductive Biology
- Plant Molecular Biology Research
- Microbial Metabolic Engineering and Bioproduction
- Chromosomal and Genetic Variations
- Plant Gene Expression Analysis
- biodegradable polymer synthesis and properties
- Microplastics and Plastic Pollution
- Genomics and Phylogenetic Studies
- Plant Disease Resistance and Genetics
- Photosynthetic Processes and Mechanisms
- Enzyme Catalysis and Immobilization
- GDF15 and Related Biomarkers
- Biochemical effects in animals
- Healthcare and Venom Research
- Biofuel production and bioconversion
- Analytical Chemistry and Chromatography
- Water Treatment and Disinfection
- Effects and risks of endocrine disrupting chemicals
- Cell death mechanisms and regulation
- Urological Disorders and Treatments
- PARP inhibition in cancer therapy
- Cancer Mechanisms and Therapy
- Lysosomal Storage Disorders Research
- Gene Regulatory Network Analysis
- RNA and protein synthesis mechanisms
Korea Research Institute of Bioscience and Biotechnology
2020-2025
University of Seoul
2007-2024
Korea Institute of Science and Technology
2023
University of Science and Technology
2019
Korea University of Science and Technology
2019
Andong National University
2010
Pusan National University
2007
Jeonbuk National University
2006
Isobutylene, a monomer for butyl rubber production, is traditionally obtained via the thermal cracking of natural gas. However, this route should be superseded by those that rely on chemical or biological valorization renewables to reduce our dependence fossil resources. Despite extensive research biobased rubber, its economically viable large-scale production from bioisobutylene remains underexplored. This study develops process microbial isobutanol and conversion isobutylene rubber. The...
Sebacic acid is an aliphatic ten-carbon dicarboxylic (1,10-decanedioic acid) with a variety of industrial applications. Here, we present its microbial production, purification, and polymerization.
Summary Whole‐genome annotation error that omits essential protein‐coding genes hinders further research. We developed Target Gene Family Finder ( TGFam‐Finder ), an alternative tool for the structural of containing target domain(s) interest in plant genomes. took considerably reduced run‐time and improved accuracy compared to conventional tools. Large‐scale re‐annotation 50 genomes identified average 150, 166 86 additional far‐red‐impaired response 1, nucleotide‐binding leucine‐rich‐repeat,...
The AP2/ERF gene family is a superfamily of transcription factors that are important in the response plants to abiotic stress and development. However, comprehensive research genes Solanaceae lacking.Here, we updated annotation genomes eight species, as well Arabidopsis thaliana Oryza sativa. We identified 2,195 genes, which 368 (17%) were newly identified. Based on phylogenetic analyses, observed expansion copy number these especially those belonging specific Ethylene-Responsive Factor...
The growing accumulation of plastic waste poses a pressing environmental challenge. Traditional disposal methods, such as incineration and landfilling, carry considerable health ecological risks. In response, our study presents an innovative method to repurpose mixed into valuable α,ω-diacids utilizing chemo-biological processes. We extracted pyrolysis oil, rich in hydrocarbons spanning broad range chain lengths (C7 C32), from household employed genetically engineered β-oxidation-blocked...
The plant homeodomain (PHD)-finger gene family that belongs to zinc-finger genes, plays an important role in epigenetics by regulating expression eukaryotes. However, inaccurate annotation of PHD-finger genes hinders further downstream comparative, evolutionary, and functional studies.We performed genome-wide re-annotation Arabidopsis thaliana (Arabidopsis), Oryza sativa (rice), Capsicum annuum (pepper), Solanum tuberosum (potato), lycopersicum (tomato) better understand the these species....
Transposable element (TE)-derived genes are increasingly recognized as major sources conferring essential traits in agriculturally important crops but underlying evolutionary mechanisms remain obscure. We updated previous annotations and constructed 18,744 FAR-RED IMPAIRED RESPONSE1 (FAR1) genes, a transcription factor family derived from Mutator-like elements (MULEs), 80 plant species, including 15,546 omitted annotations. In-depth sequence comparison of the gene repertoire revealed that...
Abstract Gene families divergently evolve and become adapted as different genes with specific structures functions in living organisms. We performed comprehensive structural functional analyses of Zinc-finger homeodomain (ZF-HDs), including Mini zinc-finger (MIFs) (ZHDs), displaying competitive each other. Intensive annotation updates for 90 plant genomes verified that most MIFs (MIF-Is) exhibited distinct motif compositions from ZHDs, although some (MIF-Zs) contained ZHD-specific motifs....
Introduciton: The α,ω-diamines (NH 2 -(CH ) n -NH and ω -amino fatty acids -COOH) have been widely used as building blocks in polymerindustries. Medium- to long-chain (C 8 C 18 acid monomers with amino residues are almost exclusively produced via chemical processes that generate hazardous waste induce severe environmental problems, such global warming pollution. Here, we present the construction platformstrains of Yarrowia lipolytica a cheese-ripening yeast, for direct biotransformation...
To genetically engineer the oleaginous yeast Yarrowia lipolytica for de novo production of tetraacetylphytosphingosine (TAPS), a precursor phytosphingosine, and optimization fermentation conditions high yield. We successfully constructed TAPS‐producing Y. CE3 strain by co‐expression Wickerhamomyces ciferrii‐derived acetyl transferases, Sli1p Atf2p. Next, we optimized several environmental factors including temperature, initial pH C/N ratio TAPS in shake culture. Deletion LCB4 increased...
Yarrowia lipolytica is a non-conventional, heterothallic, oleaginous yeast with wide range of industrial applications. Increasing ploidy can improve advantageous traits for applications including genetic stability, stress resistance, and productivity, but the construction knockout mutant strains from polyploid cells requires significant effort due to increased copy numbers target genes. The goal this study was evaluate effectiveness mating-type switching strategy by single-step...
파이토케미칼의 일종인 CAPE가 암세포 생장에 미치는 영향과 유전자 발현을 연구하기 위하여, 인간 대장암 세포주 HCT116에 CAPE를 처리하였다. CAPE의 처리는 농도 의존적으로 암 세포 생존율을 감소시키고, 세포사멸을 유도함을 확인하였다. CAPE에 의해 차별적으로 발현되는 유전자를 분석하기 oligo DNA microarray 실험을 수행하였다. 그 결과, <TEX>$20{\mu}M$</TEX> 24시간 동안 처리한 경우, 2배 이상 발현이 증가되는 266개, 감소되는 143개를 유전자중 3개(NAG-1, p21, GADD45A)를 선택하여, RT-PCR을 모든 유전자의 마이크로어레이 실험결과와 일치하였다. 또한, 후, NAG-1 유전자와 단백질의 확인한 mRNA 수준과 단백질 수준에서의 발현양상이 동일함을 게다가, 포함한 5개의 다른 종류의 파이토케미칼(resveratrol, genistein, daidzein, capsaicin)을 파이토케미칼에 증가됨을 이중...
Biofilms may appear as a patchy mass in some pipe sections and lead to deterioration of bacterial water quality, amplification corrosion, generation bad tastes odors. Characteristics biofilm formation were studied depending on materials, treatment processes temperature. From the results this study, whole, HPC (heterotrophic plate count) increased with lapse time decreased according decrease The highest showed CIP (cast iron pipe) material for all processes. lowest CIPP (cured place liner SSP...
본 연구에서는 대장암 세포주 모델에서 파이토케미칼 capsaicin에 의한 항 생장 활성과 유전체 수준에서의 유전자 발현 변화를 연구하였다. 그 결과, 처리한 capsaicin 농도 의존적으로 세포 생존율이 감소함을 확인하였고, capsaicin은 다양한 유전자의 유도하였다. DNA microarray 실험결과 <TEX>$100\;{\mu}M$</TEX> capsaicin의 처리에 의해 2배 이상 증가되는 103개가 확인된 반면, 발현이 감소되는 153개가 확인되었다. 중 4개(NAG-1, DDIT3, GADD45A 그리고 PCK2)를 선택하여 RT-PCR을 수행한 micorarray 실험과 일치함을 확인하였다. 또한 암 억제유전자인 p53의 증가됨을 RT-PCR과 real-time PCR 방법으로 게다가, NAG-1, DDIT3 유전자는 존재에 관계없이 PCK2 반드시 p53에 유도됨을 확인할 수 있었다. 이러한 연구는 세포주에서 항암 기전을 이해하는데 도움을 줄 것으로...
Abstract Background The plant homeodomain (PHD)-finger gene family that belongs to zinc-finger genes, plays important roles in epigenetics by regulating expression eukaryotes. However, inaccurate annotation of PHD-finger genes hinders further downstream comparative, evolutionary, and functional studies. Results We performed genome-wide re-annotation Arabidopsis , rice, pepper, potato, tomato better understand the role these species. Our investigation identified 875 which 225 (26% total) were...
The increasing amount of plastic waste has become a considerable environmental issue worldwide, but traditional methods disposal, such as by incineration or in landfills, have negative impacts on the environment and human health. Therefore, sustainable solutions are needed to address this problem. This study proposes an innovative approach upcycle mixed wastes into useful chemicals through chemo-biological processes. process involves pyrolysis polyvinyl chloride-containing produce crude oil...
Increasing plastic waste has become a considerable environmental issue worldwide. However, traditional methods of disposal, such as incineration or landfills, have negative impacts on the environment and human health, sustainable solutions are needed to address this problem. This study proposes an innovative approach upcycling mixed into useful chemicals through chemo-biological processes. For polyvinyl chloride-containing waste, was subjected conventional pyrolysis produce crude oil...