A5051728611- Plant-Microbe Interactions and Immunity
- Soybean genetics and cultivation
- Ginseng Biological Effects and Applications
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Seed Germination and Physiology
- Plant Stress Responses and Tolerance
- Advanced Proteomics Techniques and Applications
- Plant Gene Expression Analysis
- Plant nutrient uptake and metabolism
- Genomics and Phylogenetic Studies
- Metabolomics and Mass Spectrometry Studies
- Phytoestrogen effects and research
- Fungal and yeast genetics research
- Plant Pathogenic Bacteria Studies
- Plant tissue culture and regeneration
- Legume Nitrogen Fixing Symbiosis
- GABA and Rice Research
- Polysaccharides and Plant Cell Walls
- Machine Learning in Bioinformatics
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Plant Reproductive Biology
- Plant Disease Resistance and Genetics
- Plant and Biological Electrophysiology Studies
Pusan National University
2015-2024
Convergence
2016-2021
Abstract Phytohormones are central to plant growth and development. Despite the advancement in our knowledge of hormone signaling, downstream targets, their interactions upon hormones action remain largely fragmented, especially at protein metabolite levels. With an aim get new insight into effects two hormones, ethylene (ET) abscisic acid (ABA), this study utilizes integrated proteomics metabolomics approach investigate individual combined (ABA+ET) signaling soybean leaves. Targeting...
Alternative splicing (AS) contributes to diversifying and regulating cellular responses environmental conditions developmental cues by differentially producing multiple mRNA protein isoforms from a single gene. Previous studies on AS in pathogenic fungi focused profiling under limited number of conditions. We analysed profiles the rice blast fungus Magnaporthe oryzae, global threat production, using high-quality transcriptome data representing its vegetative growth (mycelia) host infection...
Pollen tube growth is essential for successful double fertilization, which critical grain yield in crop plants. Rapid alkalinization factors (RALFs) function as ligands signal transduction during fertilization. However, functional studies on RALF monocot plants are lacking. Herein, we functionally characterized two pollen-specific RALFs rice (Oryza sativa) using multiple clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9-induced loss-of-function mutants,...
MSP1 is a Magnaporthe oryzae secreted protein that elicits defense responses in rice. However, the molecular mechanism of action largely elusive. Moreover, it yet to be established whether functions as pathogen-associated pattern (PAMP) or an effector. Here, we employed TMT-based quantitative proteomic analysis cytosolic well plasma membrane proteins decipher induced signaling This approach led identification 6691 proteins, which 3049 were identified (PM), while 3642 fraction. A parallel...
Soybean processing, e.g., by soaking, heating, and fermentation, typically results in diverse metabolic changes. Herein, multivariate analysis-based profiling was employed to investigate the effects of fermentation Aspergillusoryzae or Bacillus subtilis on soybean substrates extracted at 4, 25, 55 °C. As changes for both A.oryzae B.subtilis were most pronounced °C, this temperature selected compare two microbial strategies, which shown be markedly different. Specifically, increased levels...
Seed coat color is an important attribute determining consumption of soybean seeds. Soybean cultivar Mallikong (M) has yellow seed while its naturally mutated mutant (MM), brown colored coat. We used integrated proteomics and metabolomics approach to investigate the differences between coats M MM during different stages development (4, 5, 6 weeks after flowering). 2DE profiling total proteins from three showed 178 differentially expressed spots which 172 were identified by MALDI‐TOF/TOF. Of...
Panax ginseng roots are well known for their medicinal properties and have been used in Korean Chinese traditional medicines 1000s of years. However, the value P. fruits remain poorly characterized. In this study, we an integrated biochemical, proteomics, metabolomics approach to look into fruits. DPPH (1,1-diphenyl-2-picrylhydrazyl) ABTS [2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)] assays showed higher antioxidant activities than leaves or roots. Two-dimensional gel...
Soaking of soybean seeds is a prerequisite for the production soy foods, and it has been shown that extent water absorbed during different imbibition conditions directly affects quality subsequent seed products by yet unknown mechanisms. In order to elucidate molecular changes in soaking temperatures, we performed an integrated proteomics metabolomics analysis soaked at 4, 25, 55 °C. Proteomics revealed various enzymes related carbohydrate protein hydrolysis were activated Interestingly,...
Summary Whole‐genome annotation error that omits essential protein‐coding genes hinders further research. We developed Target Gene Family Finder ( TGFam‐Finder ), an alternative tool for the structural of containing target domain(s) interest in plant genomes. took considerably reduced run‐time and improved accuracy compared to conventional tools. Large‐scale re‐annotation 50 genomes identified average 150, 166 86 additional far‐red‐impaired response 1, nucleotide‐binding leucine‐rich‐repeat,...
Depletion of abundant proteins is one the effective ways to improve detection and identification low-abundance proteins. Our previous study showed that protamine sulfate precipitation (PSP) method can deplete ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from leaf suitable for their in-depth proteome investigation. In this study, we provide evidence PSP also be effectively used depletion seed-storage (SSPs) total seed diverse legume plants including soybean, broad bean, pea, wild...
Ginseng is one of the well-known medicinal plants, exhibiting diverse effects. Its roots possess anticancer and antiaging properties are being used in medical systems East Asian countries. It grown low-light low-temperature conditions, its growth strongly inhibited at temperatures above 25°C. However, molecular responses ginseng to heat stress currently poorly understood, especially protein level.We a shotgun proteomics approach investigate effect on leaves. We monitored their photosynthetic...
This study develops differential protein profiles of soybean (Glycine max) seeds (cv. Saedanbaek and Daewon) varying in (47.9 39.2%) oil (16.3 19.7%) content using protamine sulfate (PS) precipitation method coupled with a 2D gel electrophoresis (2DGE) approach. Of 71 detected spots between Daewon Saedanbaek, 48 were successfully identified by MALDI-TOF/TOF. Gene ontology analysis revealed that up-regulated proteins largely associated nutrient reservoir activity (42.6%), which included...
Despite the significant technical advancements in mass spectrometry-based proteomics and bioinformatics resources, dynamic resolution of soybean seed proteome is still limited because high abundance storage proteins (SSPs). These SSPs occupy a large proportion total protein hinder identification low-abundance proteins. Here, we report TMT-based quantitative analysis matured filling stages seeds high-protein (Saedanbaek) low-protein (Daewon) cultivars by application two-way pre-fractionation...
Abstract The rice blast fungus Magnaporthe oryzae devastates cultivated ( Oryza sativa L.), causing extensive crop losses. Despite being a major pathogen, information on this pathosystem, especially the proteins associated with fungal pathogenicity and host defense response, is relatively scarce. Utilizing liquid chromatography‐mass spectrometry (LC–MS/MS), we identified 123 in planta‐ secreted M. leaves screened for their cell death‐inducing activity. Biochemical characterization of these...
Tomato spotted wilt virus (TSWV), transmitted by small insects known as thrips, is one of the major threats to tomato productivity across globe. In addition tomato, this infects more than 1000 other plants belonging 85 families and a cause serious concern. Very little, however, about molecular mechanism TSWV induced signaling in plants. Here, we used tandem mass tags (TMT)-based quantitative proteome approach investigate protein profiles leaves two cultivars (cv 2621 2689; susceptible...
Abstract Necrotrophic pathogen Cochliobolus miyabeanus ( C. ) causes rice brown leaf spot disease and drastically affects the yield quality of grains. However, molecular mechanism rice- remains poorly understood due to limited research conducted on this pathosystem. To elucidate , a transcriptome analysis was from in vitro planta grown miyabeanus. This led identification total 24,060 genes which 426 57 expressed were predicted encode for secretory proteins. As these specifically be nature,...