A5052582677- melanin and skin pigmentation
- Glycosylation and Glycoproteins Research
- Biochemical Analysis and Sensing Techniques
- Biochemical and Structural Characterization
- Tea Polyphenols and Effects
- Photochromic and Fluorescence Chemistry
- RNA Interference and Gene Delivery
- Phytochemicals and Antioxidant Activities
- Antimicrobial Peptides and Activities
- Plant biochemistry and biosynthesis
- Enzyme Catalysis and Immobilization
- Phytoestrogen effects and research
- Radioactive contamination and transfer
- Enzyme-mediated dye degradation
- Polydiacetylene-based materials and applications
- Nanowire Synthesis and Applications
- Dermatologic Treatments and Research
- Lignin and Wood Chemistry
- Radioactivity and Radon Measurements
- Gold and Silver Nanoparticles Synthesis and Applications
- Diabetes and associated disorders
- vaccines and immunoinformatics approaches
- Enzyme function and inhibition
- Microbial Metabolism and Applications
- Bone and Joint Diseases
Seoul National University
2013-2025
Dana-Farber Cancer Institute
2024
Ulsan National Institute of Science and Technology
2018
Pancreatic β cell therapy for type 1 diabetes is limited by low survival rate owing to physical stress and aggressive host immune response. In this study, we demonstrate a multilayer hydrogel nanofilm caging strategy capable of protecting cells from high shear reducing response interfering cell-cell interaction. Hydrogel fabricated monophenol-modified glycol chitosan hyaluronic acid that cross-link each other form nanothin film on the surface via tyrosinase-mediated reactions. Furthermore,...
Tyrosinase-mediated melanin synthesis is an essential biological process that can protect skin from UV radiation and radical species. This work reports on in situ biosynthesis of artificial native using photoactivatable tyrosinase (PaTy). The I41Y mutant Streptomyces avermitilis (SaTy) shows enzymatic activity comparable to wild-type SaTy. Y41 replaced with photocleavable o-nitrobenzyl tyrosine (ONBY) the introduction amber codon ONBY-tRNA synthetase/tRNA pairs. ONBY efficiently blocks...
Tyrosinases (TYRs) catalyze two-step consecutive oxidation reactions of monophenolic compounds. Since known TYRs have optimal pH at neutral to somewhat basic pH, they limitations be used for production catechol derivatives. In this study, we identified TYR from Burkholderia thailandensis (BtTYR), which exhibited high tyrosinase activity low pH. We determined the crystal structure BtTYR and provided structural basis regulation its in response change. At is inactivated due tight binding CAP...
The moisture barrier properties of a co-sputtered Al2O3/ZrO2 single layer and bilayer ALD-SiO2 were examined as potential candidates for OLED encapsulation. In the case single-layer, water vapor transmission rate (WVTR) showed strong correlation with normalized film density. A dramatic decrease in WVTR was observed film. deposition sequence also influenced WVTR, that ALD-first lower than Al2O3/ZrO2-first layer. This attributed to superior coverage on particles pinholes.
Many radionuclides exist in normal environment and artificial also can be detected. The (131I) are widely used for labeling compounds radiation therapy. In Korea, the radionuclide is produced at Radioisotope Production Facility (RIPF) Korea Atomic Energy Research Institute Daejeon. residents around RIPF assume that 131I detected environmental samples from RIPF. To ensure safety of residents, radioactive concentration near was investigated by monitoring along Gap River. selected geographical...
Melanin nanoparticles (MNPs) used for biomedical applications are often synthesized via the chemical auto-oxidation of catecholic monomers such as dopamine and 3,4-dihydroxyphenylalanine (DOPA) under alkaline conditions.
Bio-based polyurethane (PU) has recently drawn our attention due to the increasing interest in sustainability and risks involved with petroleum depletion. Herein, bio-based self-healing PU a novel polyol, i.e., eugenol glycol dimer (EGD), was synthesized characterized for first time. EGD designed have pairs of primary, secondary, aromatic alcohols, which all are able be urethane bond formation show antioxidant effects. incorporated into mixture prepolymer polyol (tetramethylene ether glycol)...
Several regiospecific enantiomers of hydroxy-(S)-equol (HE) were enzymatically synthesized from daidzein and genistein using consecutive reduction (four daidzein-to-equol-converting reductases) oxidation (4-hydroxyphenylacetate 3-monooxygenase, HpaBC). Despite the natural occurrence several HEs, most them had not been studied owing to lack their preparation methods. Herein, one-pot synthesis pathway 6-hydroxyequol (6HE) was developed HpaBC (EcHpaB) Escherichia coli (S)-equol-producing E....
Tyrosinase is generally known as a melanin-forming enzyme, facilitating monooxygenation of phenols, oxidation catechols into quinones, and finally generating biological melanin. As homologous form tyrosinase in plants, plant polyphenol oxidases perform the same reactions specifically toward polyphenols. Recent studies reported synthetic strategies for large scale preparation hydroxylated polyphenols, using bacterial tyrosinases rather than oxidase or other monooxygenases, by leveraging its...
When the skin is exposed to ultraviolet radiation (UV), it leads degradation of extracellular matrix (ECM) and results in inflammation. Subsequently, melanocytes are triggered induce tyrosinase-mediated melanin synthesis, protecting skin. Here, we introduce a proactive approach protect from photodamage via topical delivery Streptomyces avermitilis-derived tyrosinase (SaTy) using single-walled carbon nanotube (SWNT). Utilizing reverse electrodialysis (RED) battery, facilitated SaTy-SWNT...
Artificial Melanosome for Transdermal Delivery In article number 2103503 by Uk-Jae Lee, Nathaniel S. Hwang, Byung-Gee Kim, and co-workers, light-triggered in situ biosynthesis of artificial melanin native skin is proposed demonstrated using photoactivatable tyrosinase. Photoactivatable tyrosinase synthesized with site-specific incorporation o-nitrobenzyl tyrosine then encapsulated into lipid nanoparticles as an melanosome transdermal delivery. This biomimetic system can recapitulate the...
T cells play a crucial role in antitumor immune responses and the clearance of infected cells. They identify their targets through binding T-cell receptors (TCRs) to peptide–major histocompatibility complex (pMHC) molecules present cancer cells, antigen-presenting This interaction is often weak, requiring multimeric pMHC enhance avidity for identifying antigen-specific Current exchangeable pMHC-I tetramerization methods may overlook TCRs recognizing less stable yet immunogenic peptides. In...