A5031231278- Cardiac Valve Diseases and Treatments
- Cardiovascular Function and Risk Factors
- Infective Endocarditis Diagnosis and Management
- Enzyme Catalysis and Immobilization
- Advanced biosensing and bioanalysis techniques
- Polymer Surface Interaction Studies
- Enzyme Production and Characterization
- Cardiac Ischemia and Reperfusion
- Biofuel production and bioconversion
- Micro and Nano Robotics
- Electrochemical sensors and biosensors
- Laser Material Processing Techniques
- Molecular Junctions and Nanostructures
- Laser Design and Applications
- Advanced Materials and Mechanics
- Pickering emulsions and particle stabilization
- Modular Robots and Swarm Intelligence
- Solid State Laser Technologies
- Nanoparticle-Based Drug Delivery
- Coronary Interventions and Diagnostics
- Advanced Glycation End Products research
- Glass properties and applications
- Cardiac Fibrosis and Remodeling
- Diet and metabolism studies
- Graphene and Nanomaterials Applications
Children's Hospital of Philadelphia
2020-2022
Valve (United States)
2022
University of Georgia
2014-2021
Clarkson University
2013-2014
Scientific and Production Association. S.A. Lavochkin
2007
Lomonosov Moscow State University
1991
Detection of specific mRNA in living cells has attracted significant attention the past decade. Probes that can be easily delivered into and activated at desired time contribute to understanding translation, trafficking degradation mRNA. Here we report a new strategy termed magnetic field-activated binary deoxyribozyme (MaBiDZ) sensor enables both efficient delivery temporal control sensing by field. MaBiDZ uses two species beads conjugated with different components multicomponent (DZ)...
Abstract The enzymogel nanoparticle made of a magnetic core and polymer brush shell demonstrates novel type remote controlled phase‐boundary biocatalysis that involves remotely directed binding to engulfing insoluble substrates, high mobility, stability the catalytic centers. mobile enzymes reside in scaffold shuttle between interior surface engulfed substrate bioconversion process. Biocatalytic activity is preserved while brush‐like architecture favors efficient interfacial interaction when...
Biocatalytic reactions operating in parallel and resulting reduction of NAD(+) or oxidation NADH were used to mimic 3-input majority minority logic gates, respectively. The substrates corresponding the enzyme as input signals. When signals applied at their high concentrations, defined 1 values, biocatalytic activated, changes concentration output signal. dependent on number activated by absence substrates, meaning 0 kept mute with no concentration. In system mimicking function,...
A method for the generation of remotely reconfigurable anisotropic coatings is developed. To form these coatings, locking magnetic nanoparticles (LMNPs) made a superparamagnetic core and two‐component polymer shell are employed. Two different polymers phase‐separated coaxial shells. The outer provides repulsive interactions between LMNPs while inner exerts attractive forces particles. Applying non‐uniform field, one gathers particles together, pushing them to come in contact when internal...
Abstract An array of four independently wired indium tin oxide (ITO) electrodes was used for electrochemically stimulated DNA release and activation DNA‐based Identity, AND XOR logic gates. Single‐stranded molecules were loaded on the mixed poly( N , ‐dimethylaminoethyl methacrylate) (PDMAEMA)/poly(methacrylic acid) (PMAA) brush covalently attached to ITO electrodes. The deposition performed at pH 5.0 when polymer is positively charged due protonation tertiary amino groups in PDMAEMA, thus...
Theranostics of cancer using smart biocompatible materials can enable early diagnostics and treatment. Here, we report on a DNA-nanoparticle functional material, which simultaneously the presence an mRNA biomarker trigger its degradation in cultured cells. The nanodevice consists two species magnetic beads, each is conjugated with different components multicomponent deoxyribozyme (DZ) sensor. system activated only under conditions: (i) specific target (ii) when field applied. We demonstrate...
Valvular heart diseases are associated with significant cardiovascular morbidity and mortality, often require surgical and/or percutaneous repair or replacement. Valve replacement is limited to mechanical biological prostheses, the latter of which circumvent need for lifelong anticoagulation but subject structural valve degeneration (SVD) failure. Although calcification heavily studied, noncalcific SVD, represent roughly 30% BHV failures, relatively underinvestigated. This original work...
A new biomimetic nanoreactor design, MaBiDz, is presented based on a copolymer brush in combination with superparamagnetic nanoparticles. This cellular features two species of magnetic particles, each functionalized components binary deoxyribozyme system. In the presence target mRNA analyte and field, assembled to form biocompartment enclosed by polymeric that enables catalytic function enhanced kinetics. MaBiDz was demonstrated here as sensor for rapid detection imaging biomarker metastatic...
Magnetic nanoparticles (MNPs) functionalized with various enzymes (amyloglucosidase, glucose oxidase and horseradish peroxidase) were used to perform biocatalytic cascades in two different states, solute suspension or aggregated, produced the absence presence of an external magnetic field. The reactions proceeded through bulk solution diffusion intermediate substrates substrate channeling, when systems dispersed respectively. both pathways have shown very similar kinetics, unless was...
Significance Heart valve disease affects millions, and since there is no effective medical therapy, surgical repair when possible—or more commonly, replacement—are the only treatments available. Bioprosthetic heart valves (BHV), most frequently used replacements, are composed of heterograft tissue, typically fixed in glutaraldehyde, prepared for human use either as surgically implantable devices or transcatheter delivery. Protein-glycation limits durability BHV, contributing to device...
Bioprosthetic heart valves (BHV) fabricated from heterograft tissue, such as glutaraldehyde pretreated bovine pericardium (BP), are the most frequently used valve replacements. BHV durability is limited by structural degeneration (SVD), mechanistically associated with calcification, advanced glycation end products (AGE), and serum protein infiltration. We investigated hypothesis that anti-AGE agents, Aminoguanidine, Pyridoxamine [PYR], N-Acetylcysteine could mitigate AGE-serum SVD mechanisms...
This work reports on the development of biomimetic polymeric cellulosomes—enzyme–polymer conjugates (EPCs) generated by covalent binding cellulase enzymes onto a molecular brush polymer scaffold synthesized radical copolymerization poly(ethylene glycol) methyl ether methacrylate (PEGMA) and glycidyl (GMA). In contrast to natural cellulosomes, EPC is in random process conjugation different cellulases from their mixture solution. The catalytic activity EPCs systematically studied using...
Abstract The enzymogel nanoparticle made of a magnetic core and polymer brush shell demonstrates novel type remote controlled phase‐boundary biocatalysis that involves remotely directed binding to engulfing insoluble substrates, high mobility, stability the catalytic centers. mobile enzymes reside in scaffold shuttle between interior surface engulfed substrate bioconversion process. Biocatalytic activity is preserved while brush‐like architecture favors efficient interfacial interaction when...
Bioprosthetic heart valves (BHV), made from glutaraldehyde-fixed xenografts, are widely used for surgical and transcatheter valve interventions but suffer limited durability due to structural degeneration (SVD). We focused on metabolic syndrome (MetS), a risk factor SVD highly prevalent phenotype in patients affected by valvular disease with well-recognized cluster of comorbidities. Multicenter patient data (N = 251) revealed that MetS were at significantly higher accelerated required BHV...
This paper discusses various methods of increasing the efficiency controllable thermal cleavage insulating materials (oxide glasses, quartz, sapphire, etc.) by means radiation CO2 lasers: noncontact production microdefects for setting up a microcrack in material being processed, control motion laser to make it possible increase both speed and thickness high-accuracy cutting an cleavage, mutually intersecting directions obtain miniature items. © 2004 Optical Society America
Abstract Background Bioprosthetic heart valves (BHV) are widely used to treat valve disease but fundamentally limited by structural degeneration (SVD). Non-calcific mechanisms of SVD entirely account for approximately 30% cases and contribute calcific remain understudied. Glycation have not been previously associated with SVD, despite being established as degenerative in collagenous native tissues. Objectives To determine whether blood component infiltration-based glycation concomitant human...
The procedure for action in cases of a threat or an accident at oil pipeline transport facilities is defined the relevant plans emergency spillage prevention and response – ESPR. purpose article to analyze requirements legislation field development approval specifics ESRP trunk Russia are noted, US experience combating products presented. problematic issues ESRP, domestic system main transport, have been considered. It was established that Federal Law No. 207-FL, adopted July 2020,...
Introduction: Bioprosthetic heart valves (BHV) fabricated from glutaraldehyde treated bovine pericardium (BP) are widely used to treat valve disease. BHV dysfunction structural degeneration (SVD) develops over time, often necessitating device replacement. SVD is associated with both calcification and the accumulation of advanced glycation end products (AGE) serum proteins within leaflets. Here, we introduce a new strategy for mitigating AGE-associated by modifying BP...