A5064178205- Physiological and biochemical adaptations
- Neurobiology and Insect Physiology Research
- Venomous Animal Envenomation and Studies
- Crustacean biology and ecology
- Fish biology, ecology, and behavior
- Cellular transport and secretion
- Photoreceptor and optogenetics research
- Zebrafish Biomedical Research Applications
- Medical and Biological Ozone Research
- Cellular Mechanics and Interactions
- Melanoma and MAPK Pathways
- Environmental Toxicology and Ecotoxicology
- Marine Toxins and Detection Methods
- Aquaculture disease management and microbiota
- Nitric Oxide and Endothelin Effects
- Marine Invertebrate Physiology and Ecology
- Cephalopods and Marine Biology
- Antimicrobial Peptides and Activities
- Ion channel regulation and function
- Animal testing and alternatives
- Fish Biology and Ecology Studies
- Biochemical and Structural Characterization
- Computational Drug Discovery Methods
- Lipid Membrane Structure and Behavior
- Yersinia bacterium, plague, ectoparasites research
Universidade Federal do Rio Grande
2010-2024
Universidade Federal do Rio Grande do Sul
2022-2024
Universidade de São Paulo
2005-2009
Chromatic adaptation in crustaceans results from the differential distribution of colored pigment granules within their chromatophores consequent to cell signaling by neurosecretory peptides. However, force transducing, mechanochemical protein motors responsible for granule translocation, and molecular mechanisms action, are not well understood. The present study uses immunocytochemical techniques a motility assay vitro demonstrate that kinesin myosin superfamilies stably associated with...
We provide ultrastructural and cytological evidence that the tentacles of sea anemone Bunodosoma cangicum does not contain cytotoxic venom. However, we show stimulated secretion an apparent mixture biomolecules containing polypeptides from columnar vesicles is apparently a potent inducer apoptosis in zebrafish cell line, ZF-L. Microscopic fluorescence, morphology flow cytometric assays confirm apoptotic activity. Crude vesicle venom was partially purified by size exclusion chromatography....
A model for intracellular transport of pigment granules in the red ovarian chromatophores freshwater shrimp Macrobrachium olfersi is proposed on basis shifts equilibrium resting forces acting an elastic matrix. The describes a pigment-transport mechanism which mechanochemical protein motors like kinesin and myosin alternately stretch compress structurally unified, Quantifiable properties spring-matrix obey Hooke's Law during rapid phases aggregation dispersion. spring-like response mass...
Resumo As Boas Práticas de Laboratório (BPL) são um sistema controle qualidade gerencial que abrange o processo organizacional e as condições sob quais os estudos não clínicos saúde meio ambiente desenvolvidos. Conforme a Organização Mundial da Saúde (OMS) BPL devem conter cinco tópicos: recursos, caracterização, regras, resultados qualidade. O objetivo deste trabalho foi apresentar uma revisão conforme padrão OMS para implementação das em biotério zebrafish. Considerando promoção única...
Abstract The purpose of this study was to verify the occurrence pigment dispersion in retinal cells exposed UVA and UVB radiation, investigate possible participation a nitric oxide (NO) pathway. Retinal from Neohelice granulata were obtained by cellular dissociation. Cells analyzed for 30 min dark (control) then 1.1 3.3 J cm −2 UVA, 0.07 0.9 UVB, 20 n m β‐PDH (pigment dispersing hormone) or 10 μ SIN‐1 (NO donor). Histological analyses performed UV effect vivo . Cultured 250 L‐NAME synthase...
The physiological variations during the crustacean molting cycle have intrigued researchers for many years. Maintaining osmotic homeostasis in face of hemolymph dilution and dealing with dynamic intracellular extracellular calcium fluctuations are challenges these animals continuously confront. It has recently been shown that water channels present cell membrane (aquaporins) essential uptake premolt postmolt. This study aims to investigate whether hypoosmotic shock can lead translocation...
The crustacean molting cycle is triggered by the elevation of ecdysteroid levels in hemolymph during late pre‐molt. It known that these animals absorb water through intestine and gills to promote bodily swelling rupture old exoskeleton. participation two membrane proteins responsible for most uptake pre‐molt has been shown gill gut cells freshwater shrimp Palaemon argentinus : Na + /K ‐ATPase (NKA), which generates an osmoionic gradient, Aquaporins (AQPs), channels, provide higher...
We have adapted an actin-myosin motility assay to examine the interactions in vitro between actin cables isolated from giant internodal cells of freshwater alga, Nitella, and pigment granules extracted red ovarian chromatophores palaemonid shrimp, Macrobrachium olfersi. The chromatophore mass consists large |$\left( {0.{\text{5}} - {\text{1}}.0 \mu {\text{m diameter}}} \right)$| membrane-bounded granules, small {{\text{14}}0 {\text{nm \right)$|, amembranous both structurally continuous with...
Abstract Good Laboratory Practice (GLP) is a management quality control system that encompasses the organizational process and conditions under which non-clinical health environmental studies are carried out. According to World Health Organization, GLP must contain five topics: resources, characterization, rules, results, control. This work aims address review according WHO standards of implementing Practices in zebrafish (Danio rerio) vivariums. Considering promotion one (animal, human,...
In this study we provide new evidence that the columnar vesicles of sea anemone Bunodosoma cangicum are toxic in vivo and contain at least two active polypeptides, a neurotoxic an apoptosis inducing polypeptide. Here show it is also effective inducer nematode Caenorhabditis elegans. addition, peptides rapidly paralyze C. elegans, set motion sequence events result complete dissolution internal organs adult animals within 60 min. Nematodes survive toxin treatment exhibit decreased reproductive...