A5020519102- Algal biology and biofuel production
- DNA Repair Mechanisms
- DNA and Nucleic Acid Chemistry
- RNA Interference and Gene Delivery
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- CRISPR and Genetic Engineering
- Biocrusts and Microbial Ecology
- Photosynthetic Processes and Mechanisms
- RNA modifications and cancer
- Marine and coastal ecosystems
- 3D Printing in Biomedical Research
- Epigenetics and DNA Methylation
- Aquatic Ecosystems and Phytoplankton Dynamics
- Legume Nitrogen Fixing Symbiosis
Czech Academy of Sciences, Institute of Microbiology
2015-2022
Czech Academy of Sciences
2014-2021
University of South Bohemia in České Budějovice
2014-2017
Czech Academy of Sciences, Global Change Research Institute
2015
Light is the essential energy source for autotrophically growing organisms, including microalgae. Both light intensity and quality affect cell growth biomass composition. Here we used three green algae—Chlamydomonas reinhardtii, Desmodesmus quadricauda, Parachlorella kessleri—to study effects of different intensities spectra on their growth. Cultures were grown at (100, 250, 500 µmol m−2 s−1) sources: fluorescent lamps, RGB LEDs, white LEDs. quadricauda kessleri saturated 250 s−1, further...
Multiple fission is a cell cycle variation leading to the production of more than two daughter cells. Here, we used synchronized cultures chlorococcal green alga Parachlorella kessleri study its growth and pattern division under varying light intensities. The time courses DNA replication, nuclear cellular division, size, total RNA, protein content, dry matter accumulation starch were observed at incident intensities 110, 250 500 µmol photons m−2s−1. Furthermore, studied effect deuterated...
Green algae are fast-growing microorganisms that considered promising for the production of starch and neutral lipids, chlorococcal green alga Parachlorella kessleri is a favorable model, as it can produce both lipids. P. commonly divides into more than two daughter cells by specific mechanism—multiple fission. Here, we used synchronized cultures to study effects supra-optimal temperature. Synchronized were grown at optimal (30 °C) (40 temperatures incident light intensities 110 500 μmol...
The aim of the study was to verify hypothesis that a potential cause phytotoxicity diclofenac (DCF, non-steroidal anti-inflammatory drug) is an effect cell cycle progression. This research conducted using synchronous cultures model organism, green alga Chlamydomonas reinhardtii. project examined DCF effects on selected parameters characterize progression, such as size, attainment commitment points, DNA replication, number nuclei formed during cells division and morphology in consecutive...
Type I restriction-modification enzymes are multifunctional heteromeric complexes with DNA cleavage and ATP-dependent translocation activities located on motor subunit HsdR. Functional coupling of is a hallmark the restriction systems that consistent their proposed role in horizontal gene transfer. occurs at nonspecific sites distant from cognate recognition sequence, apparently triggered by stalled translocation. The X-ray crystal structure complete HsdR E. coli plasmid R124 suggested...
Type I restriction-modification enzymes are multisubunit, multifunctional molecular machines that recognize specific DNA target sequences, and their multisubunit organization underlies multifunctionality. EcoR124I is the archetype of family IC composed three subunit types: HsdS, HsdM, HsdR. cleavage ATP-dependent translocation activities housed in distinct domains endonuclease/motor Because multiple functions integrated this large 1,038 residues, a number interdomain contacts might be...
Type I restriction-modification enzymes are multisubunit, multifunctional molecular machines that recognize specific DNA target sequences, and their multisubunit organization underlies multifunctionality. EcoR124I is the archetype of family IC composed three subunit types, HsdS, HsdM, HsdR. cleavage ATP-dependent translocation activities housed in distinct domains endonuclease/motor Because multiple functions integrated this large 1038 residues, a number interdomain contacts might be...
Type I restriction-modification enzymes are multisubunit, multifunctional molecular machines that recognize specific DNA target sequences, and their multisubunit organization underlies multifunctionality. EcoR124I is the archetype of family IC composed three subunit types, HsdS, HsdM, HsdR. cleavage ATP-dependent translocation activities housed in distinct domains endonuclease/motor Because multiple functions integrated this large 1038 residues, a number interdomain contacts might be...