A5048013357- Neuroscience and Neuropharmacology Research
- Cellular transport and secretion
- Microtubule and mitosis dynamics
- Lipid Membrane Structure and Behavior
- Photoreceptor and optogenetics research
- Electronic Health Records Systems
- Ion channel regulation and function
- Cellular Mechanics and Interactions
- Protist diversity and phylogeny
- Erythrocyte Function and Pathophysiology
- Neural dynamics and brain function
- Barrier Structure and Function Studies
- Colorectal Cancer Surgical Treatments
- Marine Invertebrate Physiology and Ecology
- Advanced Electron Microscopy Techniques and Applications
- Healthcare Technology and Patient Monitoring
- Colorectal and Anal Carcinomas
- Colorectal Cancer Treatments and Studies
- Photosynthetic Processes and Mechanisms
- Neuroscience and Neural Engineering
- Retinal Development and Disorders
- Mitochondrial Function and Pathology
- Food Allergy and Anaphylaxis Research
- Planarian Biology and Electrostimulation
- American Constitutional Law and Politics
Vanderbilt University Medical Center
2021-2025
Drexel University
2024
Leidsche Rijn Julius Health Centers
2024
Center For Medicare Advocacy
2024
National Institute of Neurological Disorders and Stroke
2014-2023
National Institutes of Health
2014-2023
VA Tennessee Valley Healthcare System
2023
Vanderbilt University
2021-2022
University of Utah
2015-2021
Boston University
1990-2021
Horseradish peroxidase was administered to mice by intravenous injection, and its distribution in cerebral cortex studied with a recently available technique for localizing the electron microscope. Brains were fixed either immersion or vascular perfusion 10–60 min after administration of various doses peroxidase. Exogenous localized lumina blood vessels some micropinocytotic vesicles within endothelial cells; none found beyond endothelium. Micropinocytotic few number did not appear transport...
Certain junctions between ependymal cells, astrocytes, and some electrically coupled neurons have heretofore been regarded as tight, pentalaminar occlusions of the intercellular cleft. These are now redefined in terms their configuration after treatment brain tissue uranyl acetate before dehydration. Instead a median dense lamina, they bisected by gap 20–30 A wide which is continuous with rest interspace. The patency these "gap junctions" further demonstrated penetration horseradish...
When the nerves of isolated frog sartorius muscles were stimulated at 10 Hz, synaptic vesicles in motor nerve terminals became transiently depleted. This depletion apparently resulted from a redistribution rather than disappearance vesicle membrane, since total amount membrane comprising these remained constant during stimulation. At 1 min stimulation, 30% was nearly balanced by an increase plasma suggesting that rapidly moved to surface as it might if released their content transmitter...
We describe the design and operation of a machine that freezes biological tissues by contact with cold metal block, which incorporates timing circuit stimulates frog neuromuscular junctions in last few milliseconds before thay are frozen. show freeze-fracture replicas nerve terminals frozen during transmitter discharge, display synpatic vesicles caught act exocytosis. use 4-aminopyridine (4-AP) to increase number quanta discharged each impulse, exocytotic quick-freezing increases...
Abstract The meninges of various mammals were prepared for examination with the electronmicroscope by thin sectioning or freeze‐fracturing. Particular attention was given to distribution tight junctions in order determine basis meningeal barrier between blood circulating dural vessels and cerebrospinal fluid subarachnoid space. While some are fenestrated, those space not their component endothelial cells joined an extensive system junctions. An continuous also found a layer specialized at...
Vesicular organelles in axons of nerve cells are transported along microtubules either toward their plus ends (fast anterograde transport) or minus (retrograde transport). Two microtubule-based motors were previously identified by examining plastic beads induced to move cytosol fractions from the squid giant axon: (i) an motor, kinesin, and (ii) a retrograde which is characterized here. The cytosolic protein termed HMW1, was purified optic lobes extruded axoplasm nucleotide-dependent...
Olfactory epithelium from the frog was examined in living state by light microscopy and fixed electron microscopy. Particular attention paid to layer of cilia mucus which covers surface epithelium. The olfactory differed typical that they (a) arose bipolar neurons had centrioles near their basal bodies, (b) were up 200 microns length, greater part a distal segment containing an atypical array ciliary fibers, (c) often immotile, (d) segments arranged parallel rows mucus, (e) many vesicles...
Abstract Hyperosmotic solutions of 3 M urea, either infused into one internal carotid artery or applied topically to the pia mater rabbits, results in opening endothelial tight junctions through which horseradish peroxidase passes from blood extracellular fluid brain. The evidence for this blood‐brain barrier protein is entry pools between successive junctions. In animals not receiving interjunctional are inaccessible proteins. Having passed junctions, spreads along channels perivascular...
Much is known about the composition and function of postsynaptic density (PSD), but less its molecular organization. We use EM tomography to delineate organization PSDs at glutamatergic synapses in rat hippocampal cultures. The core PSD dominated by vertically oriented filaments, ImmunoGold labeling shows that PSD-95 a component these filaments. Vertical filaments contact two types transmembrane structures whose sizes positions match those glutamate receptors intermesh with horizontally...
PSD-95, a membrane-associated guanylate kinase, is the major scaffolding protein in excitatory postsynaptic density (PSD) and potent regulator of synaptic strength. Here we show that PSD-95 an extended configuration positioned into regular arrays vertical filaments contact both glutamate receptors orthogonal horizontal elements layered deep inside PSD rat hippocampal spine synapses. RNA interference knockdown leads to loss entire patches material, electron microscopy tomography shows patchy...
Significance The postsynaptic density (PSD) at the glutamatergic excitatory synapse is a macromolecular machine that underlies synaptic transmission and information storage. Membrane-associated guanylate kinases (MAGUKs), major scaffolding proteins PSD, are positively correlated with maturation strength, but how MAGUKs sustain strength of remains unclear. Here, we remove three MAGUK from neurons find significant reductions in by AMPARs NMDARs concomitant reduction PSD sizes core scaffold...
Importance The 21st Century Cures Act Final Rule mandates the immediate electronic availability of test results to patients, likely empowering them better manage their health. Concerns remain about unintended effects releasing abnormal patients. Objective To assess patient and caregiver attitudes preferences related receiving immediately released through an online portal. Design, Setting, Participants This large, multisite survey study was conducted at 4 geographically distributed academic...
Calcium/calmodulin-dependent protein kinase II (CaMKII) is a leading candidate for synaptic memory molecule because it persistently activated after long-term potentiation (LTP) induction and mutations that block this persistent activity prevent LTP learning. Previous work showed stimulation causes rapidly reversible translocation of CaMKII to the region. We have now measured green fluorescent (GFP)-CaMKIIα into spines during NMDA receptor-dependent chemical (cLTP) find under these...