A5091462015- Mitochondrial Function and Pathology
- Photosynthetic Processes and Mechanisms
- ATP Synthase and ATPases Research
- RNA and protein synthesis mechanisms
- Algal biology and biofuel production
- Origins and Evolution of Life
- Lipid metabolism and biosynthesis
- Metabolism and Genetic Disorders
- Endoplasmic Reticulum Stress and Disease
- Lipid Membrane Structure and Behavior
- RNA modifications and cancer
- Microbial metabolism and enzyme function
- Advanced biosensing and bioanalysis techniques
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Radioactive element chemistry and processing
- Biocrusts and Microbial Ecology
- Biotin and Related Studies
- Fluorine in Organic Chemistry
- Fungal and yeast genetics research
- Folate and B Vitamins Research
- Bacteriophages and microbial interactions
- 14-3-3 protein interactions
- Microtubule and mitosis dynamics
- RNA Interference and Gene Delivery
University of Freiburg
2023-2024
University of California, Davis
2017-2023
Abstract The presequence translocase of the mitochondrial inner membrane (TIM23) represents major route for import nuclear-encoded proteins into mitochondria 1,2 . About 60% more than 1,000 different are synthesized with amino-terminal targeting signals, termed presequences, which form positively charged amphiphilic α-helices 3,4 TIM23 sorts or matrix. Various views, including regulatory and coupling functions, have been reported on essential subunit Tim17 (refs. 5–7 ). Here we mapped...
Polycistronic gene expression, common in prokaryotes, was thought to be extremely rare eukaryotes. The development of long-read sequencing full-length transcript isomers (Iso-Seq) has facilitated a reexamination that dogma. Using Iso-Seq, we discovered hundreds examples polycistronic expression nuclear genes two divergent species green algae: Chlamydomonas reinhardtii and Chromochloris zofingiensis Here, employ range independent approaches validate multiple proteins are translated from for...
The majority of mitochondrial precursor proteins are imported through the Tom40 β-barrel channel translocase outer membrane (TOM). sorting and assembly machinery (SAM) is essential for protein insertion into thus required TOM complex. Here, we demonstrate that α-helical Mco6 co-assembles with distribution morphology Mdm10 as part SAM machinery. MCO6 MDM10 display a negative genetic interaction, mco6-mdm10 yeast double mutant displays reduced levels Cells lacking affect show defects Thus,...
The degree of residual structure retained by proteins while passing through biological membranes is a fundamental mechanistic question protein translocation. Proteins are generally thought to be unfolded transported canonical proteinaceous translocons, including the translocons outer and inner chloroplast envelope (TOC TIC). Here, we readdressed issue found that TOC/TIC accommodated tightly folded dihydrofolate reductase (DHFR) in complex with its stabilizing ligand, methotrexate (MTX). We...
Protein import into chloroplasts is carried out by the protein translocons at outer and inner envelope membranes (TOC TIC). Detailed structures for these are lacking, with only a low-resolution TOC complex structure available. Recently, we showed that TOC/TIC can folded proteins, rather unique feat coupled double membrane system. We also determined maximum functional pore size to be 30-35 Å. Here, discuss how such large pores could form compare structural dynamics of pore-forming Toc75...
Abstract The Tat pathway is essential for photosynthetic protein transport across plant thylakoid membranes and also ubiquitous throughout prokaryotes archaea. unique amongst translocation pathways as it specializes in transporting folded proteins driven by a proton motive force. Mechanistic details of the actual step (s) remain elusive. Here, we show that membrane thinning stimulates and, conversely, strengthening abolishes transport. We draw parallels from mechanism to cell penetrating...
Chloroplasts are the organelles in green plants responsible for carrying out numerous essential metabolic pathways, most notably photosynthesis. Within chloroplasts, thylakoid membrane system houses all photosynthetic pigments, reaction center complexes, and of electron carriers, is light-dependent ATP synthesis. Over 90% chloroplast proteins encoded nucleus, translated cytosol, subsequently imported into chloroplast. Further protein transport or across utilizes one four translocation...
ABSTRACT The degree of residual structure retained by proteins while passing through biological membranes is a fundamental mechanistic question protein translocation. Proteins are generally thought to be unfolded transported canonical proteinaceous translocons, which has historically been the for translocons outer and inner chloroplast envelope (TOC TIC). Here, we readdressed issue found that medium-sized tightly folded such as 22 kDa dihydrofolate reductase (DHFR) can tolerated TOC TIC....