Abstract Aphids use chemical cues to locate hosts and find mates. The vetch aphid Megoura viciae feeds exclusively on the Fabaceae, whereas currant-lettuce Nasonovia ribisnigri alternates between Grossulariaceae Asteraceae. Both species alarm pheromones warn of dangers. For N. this pheromone is a single component ( E )-β-farnesene but M. uses mixture )-β-farnesene, (−)-α-pinene, β-pinene, limonene. Odorant-binding proteins (OBP) are believed capture transport such semiochemicals their...

Abstract This review describes the main characteristics of odorant‐binding proteins ( OBP s) for homology modelling and presents a summary structure prediction studies on insect s, along with steps involved some limitations improvements. The technique involves computing approach to model protein structures is based comparison between target (unknown structure) one or more templates (experimentally determined structures). As targets prediction, s are considered play functional role...

During their lives, insects must cope with a plethora of chemicals, which few will have an impact at the behavioral level. To detect these use several protein families located in main olfactory organs, antennae. Inside antennae, odorant-binding proteins (OBPs), as most studied family, bind volatile chemicals to transport them. Pheromone-binding (PBPs) and general-odorant-binding (GOPBs) are two subclasses OBPs evolved moths putative role. Predictions for OBP-chemical interactions remained...

Acetylcholinesterase is an important target for control of neurodegenerative diseases causing cholinergic signaling deficit. Traditionally, galanthamine has been used as Amaryllidaceae-derived acetylcholinesterase inhibitor, although new Amaryllidaceae plants could serve source better inhibitors. Therefore, the objective this study was to characterize alkaloid composition from bulbs Rhodolirium andicola (Poepp.) Traub, a native Chilean specie, and assess their inhibitory activity on by in...

Abstract Migratory insects are capable of actively sustaining powered flight for several hours. This extraordinary phenomenon requires a highly efficient transport system to cope with the energetic demands placed on muscles. Here, we provide evidence that role hydrophobic ligand binding odorant proteins (OBPs) extends beyond their typical function in olfactory support insect activity via lipid interactions. Transcriptomic and candidate gene analyses show two phylogenetically clustered OBPs...

Hylamorpha elegans(Burmeister) is a native Chilean scarab beetle considered to be relevant agricultural pest pasture and cereal small fruit crops. Because of their cryptic habits, control with conventional methods difficult; therefore, alternative environmentally friendly strategies are highly desirable. The study proteins that participate in the recognition odorants, such as odorant-binding (OBPs), offers interesting opportunities identify new compounds potential modify behavior...

Abstract Currently, odorant‐binding proteins (OBPs) are considered the first filter for olfactory information insects and constitute an interesting target pest control. Thus, OBP (HeleOBP) from scarab beetle Hylamorpha elegans (Burmeister) was identified, ligand‐binding assays based on fluorescence in silico approaches were performed, followed by a simulated binding assay. Fluorescence showed slight most of ligands tested, including host‐plant volatiles. A high affinity obtained β‐ionone,...

It has been speculated that insect chemosensory proteins (CSPs) may have additional roles beyond olfaction. In this study, the phylogenetic and genomic analyses of CSPs cotton aphid, Aphis gossypii, revealed presence gene gain-and-loss among different aphid field populations. Differential expressions eight CSP genes were demonstrated after treatments with insecticides modes action. The expression AgosCSP5 was significantly upregulated by insecticide in a dose-dependent manner. Drosophila...

The European grapevine moth, Lobesia botrana (Denis & Schiffermüller), is a serious pest in vineyards North and South America. Mating disruption techniques have been used to control monitor L. on the basis of its sexual communication. This needs well-tuned olfactory system, which it believed that pheromone-binding proteins (PBPs) are key players transport pheromones antennae moths. In this study, selectivity PBP, named as LbotPBP1, was tested by fluorescence binding assays against 11 sex...

The greater wax moth, Galleria mellonella, is a global pest for beehives, doing damage in the larval stage. Although significant number of studies have reported on larvae and adults, to date no effective control has been implemented. In this study, we tested resistance alkaloids from Berberis microphylla, objective was identify enzymes that participate alkaloid detoxification through enzymatic assays, bioinformatics analysis qRT-PCR. Findings suggest glutathione-S-transferases (GSTs), an...

Red palm weevil (RPW), Rhynchophorus ferrugineus (Coleoptera: Curculionidae), is rapidly infesting trees (Arecaceae) in several countries, threatening coconut, date, and oil cultivations. The male-produced aggregation pheromone weevils has been reported to be secreted through the mouth rostrum, a snout-like projection key for emission dispersion. olfactory mechanisms that underlie peripheral odorant detection RPW have addressed at antennal level. However, rostrum remains unexplored. Through...

Olfaction and gustation processes play key roles in the life cycle of insects, such as finding accepting food sources, oviposition sites, mates, among other fundamental aspects insect development. In this context, chemosensory genes found sensory organs (e.g., antennae maxillary palps) are crucial for understanding behaviour, particularly phytophagous behaviour pests that attack economically important crops. An example is scarab beetle

In moths, sex pheromones play a key role in mate finding. These chemicals are transported the antennae by odorant-binding proteins (OBPs). Commonly, males encounter conspecific females; therefore, several OBPs male-biased. Less is known, however, about how olfactory system of moths has evolved toward inverse sexual communication, ie where females seek males. Therefore, objective this study was to identify profile and their expression patterns bee hive pest, Galleria mellonella, moth that...

The great wax moth, Galleria mellonella (L.), is a globally distributed pest for bee hives and, therefore, of significant importance apiculture. In this study, the antennal morphology G. was elucidated by light microscopy (LM) and scanning electron (SEM). Moreover, putative function sensilla studied in situ hybridization an odorant-binding protein (OBP)-encoding gene. Thus, OBP subclass called general (GOBP) used. Currently, GOBPs are related to transport sex pheromone volatiles insects....

Odorant-degrading enzymes (ODEs) are proposed to degrade/inactivate volatile organic compounds (VOCs) on a millisecond timescale. Thus, ODEs play an important role in the insect olfactory system as reset mechanism. The inhibition of these could incapacitate and, consequently, disrupt chemical communication, promoting and complementing integrated pest management strategies. Here, we report two novel aldehyde oxidases, AOX-encoding genes GmelAOX2 GmelAOX3, though transcriptomic analysis...