A5004612211- Lipid Membrane Structure and Behavior
- Pain Mechanisms and Treatments
- Erythrocyte Function and Pathophysiology
- Glycosylation and Glycoproteins Research
- Microfluidic and Capillary Electrophoresis Applications
- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- Blood properties and coagulation
- Pharmaceutical Economics and Policy
- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Pharmaceutical studies and practices
- Blood groups and transfusion
- Toxin Mechanisms and Immunotoxins
- Bacteriophages and microbial interactions
- Platelet Disorders and Treatments
- Health Systems, Economic Evaluations, Quality of Life
- Complement system in diseases
- Protein Interaction Studies and Fluorescence Analysis
- Botulinum Toxin and Related Neurological Disorders
- Microfluidic and Bio-sensing Technologies
- Pancreatic function and diabetes
- Advanced biosensing and bioanalysis techniques
- Statistical Methods in Clinical Trials
- Cholinesterase and Neurodegenerative Diseases
University of Copenhagen
2013-2023
Rigshospitalet
1970-2023
Copenhagen University Hospital
2023
Odense University Hospital
2015
Gaertner (United States)
2013
Pharmac
2011
Schools of Visual Arts, The Royal Danish Academy of Fine Arts
2002
Novo Nordisk (Denmark)
1991-2000
Novo Nordisk (United Kingdom)
1995
Pharmaceutical Biotechnology (Czechia)
1995
Crossed immunoelectrophoresis of Triton X-100 solubilized proteins from normal and abnormal platelets was performed with rabbit antibodies raised against platelets. In Bernard-Soulier protein 13 not detected, neither the amphiphilic (probably GP Ib) nor hydrophilic (glycocalicin) glycocalicin-related were seen when monospecific antiglycocalicin antiserum used. The most prominent precipitate, 16, platelet fibrinogen, 24 detected in two patients type I thrombasthenia, whereas one patient II...
Abstract In eight out of 22 patients with mammary carcinoma, extract tumour tissue induced inhibition autologous leukocyte migration in vitro, demonstrating a state cellular (delayed type) hypersensitivity. Non‐tumorous did not inhibit migration. The effect the carcinoma extracts was due to non‐specific toxicity, since leukocytes from matched controls affected by extracts. nine cases benign lesions, induce Preliminary results follow‐up studies some are reported.
Lectins were included in the first dimension gel crossed immunoelectrophoresis of human serum proteins. Con A resulted a retardation most glycoproteins during electrophoresis and heterogeneous forms individual could be detected. Ulex europeus lectin gave proteins higher migration velocity. Pokeweed mitogen did not interact with glycoproteins, but it lipoproteins. The method allows close study interacting components, determination binding specificities, detection minor including molecular forms.
In patients with non-insulin-dependent diabetes mellitus (NIDDM) and matched control subjects we examined the interrelationships between in vivo nonoxidative glucose metabolism oxidation muscle activities, as well immunoreactive protein mRNA levels of rate-limiting enzymes glycogen synthesis glycolysis, synthase (GS) phosphofructokinase (PFK), respectively. Analysis biopsies quadriceps from 19 NIDDM showed basal state a 30% decrease (P < 0.005) total GS activity 38% 0.001) mRNA/microgram DNA...
Whole human platelets and platelet membranes have been solubilized in 1% Triton X‐100, the proteins examined by crossed immunoelectrophoresis using rabbit antibodies raised against either whole or isolated membranes. 90% of were this extraction. About twenty immunoprecipitates observed extracts obtained from platelets, whereas normally eight seen with Albumin, factor VIII fibrinogen identified monospecific antibodies. Correlation patterns for was addition experiments, crossed‐line a mixture...
The C5b‐9 complex derived from human serum and assembled on target sheep erythrocyte membranes is a thin‐walled cylinder rimmed by an annulus at one end. total height of the 150 Å, towards which contributes 30 Å. has apparently uniform internal diameter 100 external 200 classical complement ‘rings’ visualized after lysis represent such cylinders perpendicularly oriented membranes. anchored in membrane maim located exterior glycocalyx. At sites attachment complexes, continuity bilayer...
Peripheral neuropathic pain is a severe chronic condition which may result from trauma to sensory nerves in the peripheral nervous system. The spared nerve injury (SNI) model induces symptoms of such as mechanical allodynia i.e. due tactile stimuli that do not normally provoke painful response [1]. SNI mouse involves ligation two three branches sciatic (the tibial and common peroneal nerve), while sural left intact [2]. lesion results marked hypersensitivity lateral area paw, innervated by...
Blocking of sortilin ligand binding prevents neuropathic pain by inhibiting BDNF-induced spinal KCC2 down-regulation.
The terminal, membrane-derived C5b-9 complex of human complement (C) is an apparently hollow, cylindrical macromolecule vertically oriented on the target membrane. In present study, antiserum to has been used probe its immunobiochemical properties. "Neoantigenic" determinants characteristic have detected, which are absent native C5-C9 molecules. Evidence that amphiphilic molecule possesses apolar, detergent-binding surfaces obtained by using charge-shift crossed immunoelectrophoresis, and...
Many antibody preparations exhibit proteolytic activity due to the presence of plasmin. In crossed immunoelectrophoresis at pH 8.6 this enzyme can degrade certain proteins during electrophoresis in antibody‐containing gel, resulting artefacts form extra precipitation arcs congruent shape. The degradation behaviour spectrin, a major protein human erythrocyte membranes, was investigated. artefact could be completely abolished by addition protease inhibitors, e.g. aprotinin and soya bean...
Abstract. The case history and investigations of a 32‐year‐old man with pernicious anaemia selective absence IgA in serum saliva are presented discussed. On two occasions, the patient, who had not previously been exposed to foreign human serum, developed severe anaphylactic shock after blood transfusion few millilitres. A precipitating antibody was identified his serum. It class‐specific type reacting only donors' sera, but also other normal sera. patient no apparent chromosome abnormality,...
Apart from causing the formation of y-glutamyl-elysine cross-linked polymers, exposure human erythrocytes to Ca2+ and ionophore A23181 leads a breakdown two major transmembrane proteins, ie. anion-transporting band 3 glycophorin.This apparently proteolytic phenomenon was examined by crossed immunoelectrophoretic techniques.The main product cleavage had chain weight about 55,000 showed good precipitation with antibody raised against intact protein.The degradation glycophorin more rapid and,...