A5059257212- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Ubiquitin and proteasome pathways
- Chromosomal and Genetic Variations
- Pluripotent Stem Cells Research
- Genetics and Neurodevelopmental Disorders
- Insect Resistance and Genetics
- Wnt/β-catenin signaling in development and cancer
- Protein Degradation and Inhibitors
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Tissue Engineering and Regenerative Medicine
- Sex and Gender in Healthcare
- Cancer-related gene regulation
- Insect symbiosis and bacterial influences
- Cardiomyopathy and Myosin Studies
- Genetics, Aging, and Longevity in Model Organisms
- Protein Tyrosine Phosphatases
- Invertebrate Immune Response Mechanisms
- Cell Adhesion Molecules Research
- RNA Research and Splicing
- Angiogenesis and VEGF in Cancer
- RNA and protein synthesis mechanisms
- BRCA gene mutations in cancer
Johannes Gutenberg University Mainz
2023-2025
Institute of Molecular Biology
2021-2025
University Medical Center of the Johannes Gutenberg University Mainz
2023-2024
Max Planck Institute of Immunobiology and Epigenetics
2013-2023
University of Naples Federico II
2011
Abstract In mammals, X-chromosomal genes are expressed from a single copy since males (XY) possess X chromosome, while females (XX) undergo inactivation. To compensate for this reduction in dosage compared with two active copies of autosomes, it has been proposed that the chromosome exhibit compensation. However, existence and mechanisms X-to-autosome compensation still under debate. Here we show transcripts have fewer m 6 A modifications more stable than their autosomal counterparts. Acute...
Integrins are heterodimeric receptors that play a critical role in cell-cell and cell-matrix adhesion processes. Among them, αVβ3 integrin, recognizes the aminoacidic RGD triad, is reported to be involved angiogenesis, tissue repair tumor growth. We have recently synthesized new selective ligand of receptor, referred as RGDechiHCit, contains cyclic motif two echistatin moieties.The aim this study evaluate vitro vivo effects RGDechiHCit. Therefore, we assessed its properties cellular...
Abstract Haploinsufficiency and aneuploidy are two phenomena, where gene dosage alterations cause severe defects ultimately resulting in developmental failures disease. One remarkable exception is the X chromosome, copy number differences between sexes buffered by compensation systems. In Drosophila , Male-Specific Lethal complex (MSLc) mediates upregulation of single male chromosome. The evolutionary origin conservation this process orchestrated MSL2, only male-specific protein within fly...
The Anopheles mosquito is one of thousands species in which sex differences play a central part their biology, as only females need blood meal to produce eggs. Sex differentiation regulated by chromosomes, but presence creates dosage imbalance between males (XY) and (XX). Dosage compensation (DC) can re-equilibrate the expression chromosomal genes. However, because DC mechanisms have been fully characterized few model organisms, key questions about its evolutionary diversity functional...
Hintergrund: Mitochondriale Kardiomyopathien beruhen auf einem muskulären Energiemangel, der durch Atmungskettendefekte verursacht ist. Ursächliche Veränderungen können sowohl in nukleären als auch mitochondrialen Genen zu finden sein. Das nukleär kodierte MALSU1 Protein spielt eine Rolle bei Bildung und Aufrechterhaltung großen Untereinheit mitochondrialer Ribosomen, die für Proteinbiosynthese verantwortlich sind.
Sex chromosomes impact chromatin organization and histone modification dynamics differently between males females, particularly those involved in dosage compensation (DC). The stability of DC mechanisms may shape sex-specific phenotypes traits such as lifespan. However, the tissue- age-dependent variations well evolutionary diversity are incompletely understood. Here, we investigate occurrence H4 lysine 16 acetylation (H4K16ac), previously known for its role sex chromosome male-heterogametic...
Cells rely on a diverse repertoire of genes for maintaining homeostasis, but the transcriptional networks underlying their expression remain poorly understood. The MOF acetyltransferase-containing Non-Specific Lethal (NSL) complex is broad transcription regulator. It essential in Drosophila, and haploinsufficiency human KANSL1 subunit results Koolen-de Vries syndrome. Here, we perform genome-wide RNAi screen identify BET protein BRD4 as an evolutionary conserved co-factor NSL complex. Using...
Abstract In diploid organisms, biallelic gene expression enables the production of adequate levels mRNA 1,2 . This is essential for haploinsufficient genes, which require optimal function to prevent onset developmental disorders 1,3 Whether and how a or monoallelic state determined in cell-type-specific manner at individual loci remains unclear. MSL2 known dosage compensation male X chromosome flies. Here we identify role regulating allelic mammals. Allele-specific bulk single-cell analyses...
Sex chromosomes induce potentially deleterious gene expression imbalances that are frequently corrected by dosage compensation (DC). Three distinct molecular strategies to achieve DC have been previously described in nematodes, fruit flies, and mammals. Is this a consequence of genomes, functional or ecological constraints, random initial commitment an evolutionary trajectory? Here, we study the malaria mosquito Anopheles gambiae . The Drosophila X evolved independently but share high degree...
Cadherin switching from E-cadherin (E-cad) to N-cadherin (N-cad) is a key step of the epithelial-mesenchymal transition (EMT) processes that occurs during gastrulation and cancer progression. We investigate whether cadherins actively participate in progression EMT by crosstalk signaling pathways. apply ectopic cadherin before onset mouse gastrulation. Mutants with an induced E-cad N-cad switch (Ncadki) die around E8.5. Severe morphological changes including small epiblast, rounded shape,...