A5100669355- Plant Physiology and Cultivation Studies
- Plant Molecular Biology Research
- Glycosylation and Glycoproteins Research
- Organ Donation and Transplantation
- Plant responses to water stress
- Photosynthetic Processes and Mechanisms
- Organ and Tissue Transplantation Research
- Biochemical and Molecular Research
- Autophagy in Disease and Therapy
- Organ Transplantation Techniques and Outcomes
- Tendon Structure and Treatment
- Enzyme Structure and Function
- Cancer Research and Treatments
- Medicinal Plants and Bioactive Compounds
- Bacteriophages and microbial interactions
- Protist diversity and phylogeny
- Amino Acid Enzymes and Metabolism
- Plant pathogens and resistance mechanisms
- Plant Stress Responses and Tolerance
- Anesthesia and Neurotoxicity Research
- Calcium signaling and nucleotide metabolism
- Essential Oils and Antimicrobial Activity
- Bacterial Infections and Vaccines
- Polysaccharides and Plant Cell Walls
- Knee injuries and reconstruction techniques
Anhui Agricultural University
2021-2024
University of Chinese Academy of Sciences
2023
Chinese Academy of Sciences
2020-2023
Institute of Hydrobiology
2023
State Key Laboratory of Freshwater Ecology and Biotechnology
2023
Fujian Normal University
2022
State Key Laboratory of Microbial Resources
2020
Microbiology Institute of Shaanxi
2020
Institute of Microbiology
2020
Harbin Medical University
2014-2017
Abstract The transformation and gene editing of the woody species kiwifruit are difficult time-consuming. fast marker-free genetic modification system for has not been developed yet. Here, we establish a rapid efficient mediated by Agrobacterium rhizogenes kiwifruit. Moreover, removing-root-tip method was to significantly increase regeneration efficiency transgenic hairy roots. Through A. -mediated CRISPR/Cas9 editing, efficiencies CEN4 AeCBL3 achieved 55 50%, respectively. And several...
The chloroplast is the main organelle for stress-induced production of reactive oxygen species (ROS). However, how chloroplastic ROS homeostasis maintained under salt stress largely unknown. We show that EGY3, a gene encoding chloroplast-localized protein, induced by and oxidative stresses. loss EGY3 function causes hypersensitivity while overexpression increases tolerance to both interacts with Cu/Zn-SOD2 (CSD2) promotes CSD2 stability conditions. In egy3-1 mutant plants, degradation limits...
Fruit ripening is associated with the degreening process (loss of chlorophyll) that occurs in most fruit species. Kiwifruit one special species whose fruits may maintain green flesh by accumulating a large amount chlorophyll even after ripening. However, little known about genetic variations related to process. Here, graph-based kiwifruit pangenome analyzing 14 chromosome-scale haplotype-resolved genome assemblies from seven representative cultivars or lines Actinidia chinensis built. A...
Clathrin-mediated vesicular formation and trafficking are responsible for molecular cargo transport signal transduction among organelles. Our previous study shows that CHLOROPLAST VESICULATION (CV)-containing vesicles (CVVs) generated from chloroplasts chloroplast degradation under abiotic stress. Here, we show CV interacts with the clathrin heavy chain (CHC) induces vesicle budding toward cytosol inner envelope membrane. In defective mutants of CHC2 dynamin-encoding DRP1A, CVV releasing...
Abstract Objectives To investigate the protective effect of catalpol on cerebral ischaemia/reperfusion (CI/R) injury in gerbils and further explore underlying mechanism. Methods A gerbil model CI/R was prepared by bilateral common carotid occlusion for 10 min followed 6 h reperfusion. Catalpol (5, or 20 mg/kg per day) injected intraperitoneally 3 days before occlusion. Stroke index measured during The contents endogenous neuropeptides, endothelin-1 (ET-1) calcitonin gene-related peptide...
Summary Aims The mouse model of allo‐head and body reconstruction ( AHBR ) has recently been established to further the clinical development this strategy for patients who are suffering from mortal bodily trauma or disease, yet whose mind remains healthy. Animal studies indispensable developing such novel surgical practices. goal work was establish head transplant model, then next step through feasible biological investigate immune rejection brain function in step, thereby promoting...
Abstract The mitochondrion is an important signaling hub that governs diverse cellular functions, including metabolism, energy production, and immunity. Among the hundreds of effectors translocated into host cells by Dot/Icm system Legionella pneumophila , several are targeted to mitochondria but function most them remains elusive. Our recent study found effector Ceg3 inhibits activity ADP/ATP translocases (ANTs) ADP‐ribosylation (ADPR). Here, we show effect antagonized Larg1, encoded...
Summary Aims There is still no effective way to save a surviving healthy mind when there critical organ failure in the body. The next frontier CTA allo‐head and body reconstruction ( AHBR ), just as animal models were key development of , they will be crucial establishing procedures for clinical translation. Methods results Our approach, pioneered mice, involves retaining donor brain stem transplanting recipient head. preliminary data mice support that this allows retention breathing...
Kiwifruit (Actinidia chinensis) is commonly covered by fruit hairs (trichomes) that affect kiwifruit popularity in the commercial market. However, it remains largely unknown which gene mediates trichome development kiwifruit. In this study, we analyzed two species, A. eriantha (Ae) with long, straight, and bushy trichomes latifolia (Al) short, distorted, spare trichomes, second- third-generation RNA sequencing. Transcriptomic analysis indicated expression of NAP1 gene, a positive regulator...
Growth differentiation factor (GDF)‑5 serves a role in tissue development and tenomodulin an important the of tendons. The effects GDF‑5 on mesenchymal stem cells (MSCs), particularly with regards to tendon bioengineering, are poorly understood. present study aimed investigate cell viability expression MSCs from murine compact bone. were isolated bones confirmed by flow cytometric analysis. In addition, adipogenic, osteoblastic chondrocyte capabilities determined. treated MSC mRNA protein...
Abstract Only two de novo biosynthetic routes to nicotinamide adenine dinucleotide (NAD + ) have been described, both of which start from a proteinogenic amino acid and are tightly controlled. Here we establish C3N pathway starting chorismate in Escherichia coli as third NAD biosynthesis pathway. Significantly, the yielded extremely high cellular concentrations NAD(H) E. . Its utility cofactor engineering was demonstrated by introducing four-gene module cell factories achieve higher...
Objective To optimize MDCK-siat7e cell culture conditions by response surface methodology (RSM). Methods Initial ammonia concentration, lactic acid osmotic pressure and viable density (VCD) of were used as factors. Cell specific growth rate, viability, accumulation ratios at 72 h set values. The full factorial design RSM to study the effect these factors on culture. Results During cells culture, initial VCD was a significant factor effecting rate (t=3.43, P<0.05); concentrations...