A5043502227- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Photochromic and Fluorescence Chemistry
- Tea Polyphenols and Effects
- Retinoids in leukemia and cellular processes
- Neuroscience and Neuropharmacology Research
- Glaucoma and retinal disorders
- Muscle Physiology and Disorders
- Connexins and lens biology
- Neurological Disease Mechanisms and Treatments
- Retinal and Optic Conditions
- Circadian rhythm and melatonin
- Molecular Sensors and Ion Detection
- Drug-Induced Ocular Toxicity
- Neuroscience and Neural Engineering
- Adipose Tissue and Metabolism
- Escherichia coli research studies
- Photoreceptor and optogenetics research
- Phosphodiesterase function and regulation
- Tannin, Tannase and Anticancer Activities
- Antioxidant Activity and Oxidative Stress
- Pharmacological Effects of Natural Compounds
- Neurological Disorders and Treatments
- Gut microbiota and health
- Medicinal Plants and Neuroprotection
Inserm
2002-2023
Université Clermont Auvergne
2003-2023
Clermont Université
2011-2023
Laboratoire Epidémiologie et Analyses en Santé Publique : Risques, Maladies Chroniques et Handicaps
2011-2012
Sorbonne Université
2004-2005
Hôpital Saint-Antoine
2005
Laboratoire de Météorologie Physique
1999
The aetiology of Crohn's disease (CD) involves disorders in host genetic factors and intestinal microbiota. Adherent-invasive Escherichia coli (AIEC) are receiving increased attention because studies mucosa-associated microbiota, they more prevalent CD patients than healthy subjects. AIEC associated both with ileal colonic phenotypes. In this study, we reported a protease called Vat-AIEC from that favours the mucosa colonization. deletion Vat-AIEC-encoding gene resulted an adhesion-impaired...
To determine whether the green tea polyphenol epigallocatechin gallate (EGCG) could prevent H(2)O(2)-induced oxidative stress in primary rat retinal pigment epithelial cells.Primary cultures of epithelium (RPE) cells were established from Long-Evans newborn rats. RPE pretreated with various concentrations EGCG for 24 h before being exposed to hydrogen peroxide (H(2)O(2)) 2 induce stress. Cell metabolic activity was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide...
Abstract In different animal models, photoreceptor degeneration was correlated to an abnormal increase in cGMP concentration. The cGMP‐induced toxicity demonstrated by applying the phosphodiesterase inhibitor 3‐isobutyl‐1‐methylxanthine on retinal explants. To assess role of cGMP‐gated channels this toxicity, Ca 2+ channel blockers verapamil and l ‐ d ‐diltiazem, which block with efficacies, were applied vitro models degeneration. These included: (i) adult rat explants incubated zaprinast, a...
Recent reports on rod photoreceptor neuroprotection by Ca2+ channel blockers have pointed out the need to assess effect of these mammalian rods. However, in mammals, electrophysiological characterization has been hampered small size photoreceptors, which were instead extensively studied nonmammalian vertebrates. To further characterize ionic conductances and pharmacology channels rods, freshly dissociated pig photoreceptors recorded with whole cell patch-clamp technique. Rod cells expressed...
Among retinal macular diseases, the juvenile recessive Stargardt disease and age-related degenerative arise from carbonyl oxidative stresses (COS). Both originate an accumulation of all-trans-retinal (atRAL) are involved in bisretinoid formation by condensation atRAL with phosphatidylethanolamine (carbonyl stress) photoreceptor its transformation into lipofuscin bisretinoids (oxidative pigment epithelium (RPE). As contribute to RPE cell death, our goal is select powerful chemical inhibitors...
Abstract All‐ trans ‐retinal (a t RAL) is a highly reactive carbonyl specie, known for its reactivity on cellular phosphatidylethanolamine in photoreceptor. It generated by photoisomerization of 11‐ cis chromophore linked to opsin the Schiff's base reaction. In ABCA4‐associated autosomal recessive Stargardt macular dystrophy, RAL results and oxidative stress, which leads bisretinoid A2E, accumulation retinal pigment epithelium (RPE). This A2E‐accumulation presents as lipofuscin fluorescent...
Abstract Environmental light has deleterious effects on the outer retina in human retinopathies, such as ABCA4 -related Stargardt’s disease and dry age-related macular degeneration. These involve carbonyl oxidative stress, which contribute to retinal cell death vision loss. Here, we used an albino Abca4 −/− mouse model, of shows susceptibility acute photodamage, test protective efficacy a new polyunsaturated fatty acid lipophenol derivative. Anatomical functional analyses demonstrated that...
Abstract Oxidative stress plays an important role in the ageing of retina and pathogenesis retinal diseases such as age‐related macular degeneration (ARMD). Hydrogen peroxide is a reactive oxygen species generated by photo‐excited lipofuscin that accumulates during pigment epithelium (RPE), accumulation associated with ARMD. Iron also age RPE may contribute to ARMD source oxidative stress. The aim this work was investigate effects L‐Citrulline (CIT), naturally occurring amino acid known...
<b><i>Aim:</i></b> Most Duchenne muscular dystrophy patients and the mdx<sup>Cv3</sup> mouse strain, lacking expression of both dystrophins Dp260 Dp71, show a high attenuation dark-adapted electroretinogram (ERG) b-wave amplitude, whereas mice normal amplitude. Here, we completed our assessment whether sole absence Dp71 affects ERG. <b><i>Methods:</i></b> Ganzfeld ERGs were performed on Dp71-null littermates. Scotopic flash recorded...
To evaluate the in vitro effects of bevacizumab (Avastin®) on electroretinogram (ERG) rats using a model isolated perfused retina ERG recording.Retinas were from rat eyes and placed chamber continuously with nutrient solution. The was recorded every 3 min. Once b-wave amplitude at steady state, added concentrations 0.25 0.5 mg/ml to perfusion medium for h.We observed no effect amplitudes or kinetics when medium. In addition, we found significant differences survival curves PIII wave during...
Purpose To evaluate the effects of H2O2 as an oxidant on electroretinogram (ERG) in isolated rat retina.