A5080950280- Gut microbiota and health
- Helicobacter pylori-related gastroenterology studies
- Cancer Research and Treatments
- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Autophagy in Disease and Therapy
- Inflammatory Bowel Disease
- MicroRNA in disease regulation
- Drug Transport and Resistance Mechanisms
- Amino Acid Enzymes and Metabolism
- Clostridium difficile and Clostridium perfringens research
- RNA Interference and Gene Delivery
- Pharmacological Effects of Natural Compounds
- Epigenetics and DNA Methylation
- Probiotics and Fermented Foods
- Digestive system and related health
- Adenosine and Purinergic Signaling
- Pediatric Hepatobiliary Diseases and Treatments
- NF-κB Signaling Pathways
- Salmonella and Campylobacter epidemiology
- Immunotherapy and Immune Responses
- Microscopic Colitis
- Gastrointestinal motility and disorders
- Cancer Cells and Metastasis
- Cancer-related gene regulation
Inserm
2013-2024
Microbe, Intestine, Inflammation and Host Susceptibility
2015-2024
Université Clermont Auvergne
2012-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Bambino Gesù Children's Hospital
2017-2024
Centre de Recherche en Nutrition Humaine d'Auvergne
2021
Clermont Université
2012-2021
Centre Hospitalier Universitaire de Clermont-Ferrand
2016-2021
Istituti di Ricovero e Cura a Carattere Scientifico
2017
Instituts Universitaires de Technologie
2016
<h3>Background</h3> <i>Escherichia coli</i> strains harbouring the <i>pks</i> island (<i>pks</i>+ <i>E. coli</i>) are often seen in human colorectal tumours and have a carcinogenic effect independent of inflammation an AOM/IL-10<sup>−/−</sup> (azoxymethane/interleukin) mouse model. <h3>Objective</h3> To investigate mechanism sustaining <i>pks</i>+ coli</i>-induced carcinogenesis. <h3>Method</h3> Underlying cell processes were investigated <i>in vitro</i> vivo</i> (xenograft model) using...
Background Inflammatory bowel diseases (IBD), including mainly ulcerative colitis (UC) and Crohn's disease (CD), are inflammatory disorders of the gastrointestinal tract caused by an interplay genetic environmental factors. Murine model induced Dextran Sulfate Sodium (DSS) is animal IBD that commonly used to address pathogenesis as well test efficacy therapies. In this study we systematically analyzed clinical parameters, histological changes, intestinal barrier properties cytokine profile...
The gut microbiota is suspected to promote colorectal cancer (CRC). Escherichia coli are more frequently found in CCR biopsies than healthy mucosa; furthermore, the majority of mucosa-associated E. isolated from harbors pks genomic island (pks+ coli) that responsible for synthesis colibactin, a genotoxic compound. We have recently reported transient contact few malignant cells with colibactin-producing increases tumor growth xenograft mouse model. Growth sustained by cellular senescence...
Microbiota are known to modulate host gene expression, yet the underlying molecular mechanisms remain elusive. MicroRNAs (miRNAs) importantly implicated in many cellular functions by post-transcriptionally regulating expression via binding 3'-untranslated regions (3'-UTRs) of target mRNAs. However, a role for miRNAs microbiota-host interactions remains unknown. Here we investigated if involved microbiota-mediated regulation expression. Germ-free mice were colonized with microbiota from...
Expression of the transmembrane glycoprotein CD98 (encoded by SLC3A2) is increased in intestinal inflammatory conditions, such as bowel disease (IBD), and various carcinomas, yet its pathogenetic role remains unknown. By generating gain- loss-of-function mouse models with genetically manipulated expression specifically epithelial cells (IECs), we explored homeostasis, inflammation, colitis-associated tumorigenesis. IEC-specific overexpression induced gut homeostatic defects responses to...
Colibactin-producing Escherichia coli (CoPEC) colonize the colonic mucosa of a higher proportion patients with vs without colorectal cancer (CRC) and promote carcinogenesis in susceptible mouse models CRC. Autophagy degrades cytoplasmic contents, including intracellular pathogens, via lysosomes regulates intestinal homeostasis. We investigated whether inhibiting autophagy affects mice infected CoPEC.Human epithelial cells (IECs) (HCT-116) were strain CoPEC (11G5 strain) isolated from patient...
Intratumoral bacteria flexibly contribute to cellular and molecular tumor heterogeneity for supporting cancer recurrence through poorly understood mechanisms. Using spatial metabolomic profiling technologies 16SrRNA sequencing, we herein report that right-sided colorectal tumors are predominantly populated with Colibactin-producing
Human colorectal cancers (CRCs) are readily colonized by colibactin-producing E. coli (CoPEC). CoPEC induces DNA double-strand breaks, mutations, genomic instability, and cellular senescence. Infected cells produce a senescence-associated secretory phenotype (SASP), which is involved in the increase tumorigenesis observed CRC mouse models infected with CoPEC. This study investigated whether CoPEC, SASP derived from CoPEC-infected cells, impacted chemotherapeutic resistance. intestinal...
Enterohemorrhagic Escherichia coli (EHEC) infections are associated with the modification of tight-junction permeability and synthesis proinflammatory cytokine interleukin-8 (IL-8). In a previous study, it was demonstrated that EHEC-induced IL-8 secretion is due to involvement mitogen-activated protein kinase (MAPK), AP-1, NF-kappaB pathways. this we investigated effect yeast Saccharomyces boulardii on EHEC infection in T84 cells. For purpose, cells were (i) incubated bacteria at same time...
The transmembrane glycoprotein CD98 regulates multiple cellular functions, including extracellular signaling, epithelial cell adhesion/polarity, amino acid transport, and cell-cell interactions. MicroRNAs post-transcriptionally regulate gene expression, thereby functioning as modulators of numerous processes, such differentiation, proliferation, apoptosis. Here, we investigated if microRNAs expression during intestinal differentiation inflammation. We found that microRNA-7 repressed in...
Salmonella pathogenesis engages host cells in two-way biochemical interactions: phagocytosis of bacteria by recruitment cellular small GTP-binding proteins induced the bacteria, and triggering a pro-inflammatory response through activation MAPKs nuclear translocation NF-kappaB. Worldwide interest use functional foods containing probiotic for health promotion disease prevention has increased significantly. Saccharomyces boulardii is non-pathogenic yeast used as infectious diarrhea.In this...
Colorectal cancers (CRCs) are frequently colonised by colibactin toxin-producing Escherichia coli bacteria that induce DNA damage in host cells and exhibit protumoural activities. Our objective was to identify small molecules inhibiting the toxic effects induced these colibactin-producing bacteria.A structural approach adopted for identification of a putative ligand ClbP enzyme involved synthesis colibactin. Intestinal epithelial CRC mouse model were used assess activity selected compounds...
The aetiology of Crohn's disease (CD) involves disorders in host genetic factors and intestinal microbiota. Adherent-invasive Escherichia coli (AIEC) are receiving increased attention because studies mucosa-associated microbiota, they more prevalent CD patients than healthy subjects. AIEC associated both with ileal colonic phenotypes. In this study, we reported a protease called Vat-AIEC from that favours the mucosa colonization. deletion Vat-AIEC-encoding gene resulted an adhesion-impaired...