A5056719439- Inflammatory Bowel Disease
- Gut microbiota and health
- Helicobacter pylori-related gastroenterology studies
- IL-33, ST2, and ILC Pathways
- Cancer Research and Treatments
- Diabetes and associated disorders
- Immune Cell Function and Interaction
- Viral gastroenteritis research and epidemiology
- Pancreatic function and diabetes
- Escherichia coli research studies
- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- Mycobacterium research and diagnosis
- Clostridium difficile and Clostridium perfringens research
- Colorectal Cancer Treatments and Studies
- Cancer Cells and Metastasis
- Immune Response and Inflammation
- Proteoglycans and glycosaminoglycans research
- Metabolism, Diabetes, and Cancer
- Respiratory viral infections research
- Pharmacological Effects of Natural Compounds
- Virus-based gene therapy research
- Cancer, Lipids, and Metabolism
- Digestive system and related health
- Immune responses and vaccinations
Université de Lille
2020-2024
Inserm
2001-2024
Laboratoire de Physiologie Cellulaire
2022-2024
Onco Lille
2023
Center for Infection and Immunity of Lille
2018-2022
Institut Pasteur de Lille
2020-2022
Centre National de la Recherche Scientifique
2020-2022
Centre Hospitalier Universitaire de Lille
2020-2022
John Radcliffe Hospital
2010-2013
University of Oxford
2009-2013
Patients with inflammatory bowel disease (IBD) have an increased risk of colon cancer. However, the immune cells and cytokines that mediate transition from intestinal inflammation to cancer are poorly understood. We show bacteria-induced is accompanied by differential accumulation IL-17(+)IL-22(+) colonic innate lymphoid (cILCs), which phenotypically distinct LTi NK-22 cells, their depletion in mice dysplastic blocks development invasive Analysis functional role Type 17 shows although...
Intratumoral bacteria flexibly contribute to cellular and molecular tumor heterogeneity for supporting cancer recurrence through poorly understood mechanisms. Using spatial metabolomic profiling technologies 16SrRNA sequencing, we herein report that right-sided colorectal tumors are predominantly populated with Colibactin-producing
Crohn's disease is linked to a decreased diversity in gut microbiota composition as potential consequence of an impaired anti-microbial response and altered polarization T helper cells. Here, we evaluated the immunomodulatory properties two probiotic strains, namely Bifidobacterium animalis spp. lactis Bl 5764 Lactobacillus reuteri Lr 5454 strains. Both strains improved colitis triggered by either 2,4,6-trinitrobenzenesulfonic acid (TNBS) or Citrobacter rodentium infection mice. Training...
Mutations in the nucleotide-binding oligomerization domain protein 12 (NLRP12) cause recurrent episodes of serosal inflammation. Here we show that NLRP12 efficiently sequesters HSP90 and promotes K48-linked ubiquitination degradation NOD2 response to bacterial muramyl dipeptide (MDP). This interaction is mediated by linker-region proximal NLRP12. Consequently, disease-causing R284X mutation fails repress MDP-induced NF-κB subsequent activity JAK/STAT signaling pathway. While deficiency...
Immune checkpoint inhibitors (ICI) have been positioned as a standard of care for patients with advanced non-small-cell lung carcinomas (NSCLC). A pilot clinical trial has reflected optimistic association between supplementation Clostridium butyricum MIYAIRI 588 (CBM588) and ICI efficacy in NSCLC. However, it remains to be established whether this biotherapeutic strain may sufficient heighten the immunogenicity tumor draining lymph nodes overcome resistance ICI. Herein, we report that CBM588...
Chronic inflammation of the intestine has been associated with an elevated risk developing colorectal cancer. Recent association studies have highlighted role genetic predisposition in etiology colitis and started to unravel its complexity. However, factors influencing progression from colon tumorigenesis are not known. We report identification a interval Hiccs that regulates Helicobacter hepaticus–induced cancer susceptibility 129.RAG−/− mouse model. The 1.7-Mb congenic on chromosome 3,...
Nutrition appears to be an important environmental factor involved in the onset of inflammatory bowel diseases (IBD) through yet poorly understood biological mechanisms. Most studies focused on fat content high caloric diets, while refined sugars represent up 40% intake within industrialized countries and contribute growing epidemics diseases. Herein we aim better understand impact a high-fat-high-sucrose diet intestinal homeostasis healthy conditions subsequent colitis risk. We investigated...
Background & AimsLoss-of-function variants in nucleotide-binding oligomerization domain-containing protein 2 (NOD2) impair the recognition of bacterial cell wall component muramyl-dipeptide and are associated with an increased risk for developing Crohn's disease. Likewise, exposure to antibiotics increases individual inflammatory bowel Here, we studied long-term impact NOD2 on ability gut fungal microbiota recover after antibiotic treatment.MethodsTwo cohorts 20-week-old 52-week-old...
The Idd5 locus for autoimmune diabetes in nonobese diabetic (NOD) mice has been mapped to the proximal half of chromosome 1 and appears include two loci, Idd5.1 Idd5.2, being a candidate homolog human IDDM12 locus. Using new recombinant congenic lines, we have reduced interval 5 cM at most, between D1Mit279 D1Mit19 (not included). This now excludes Casp8 Cflar (Flip) genes. It still retains Cd28 Ctla4 also includes Icos (inducible costimulator). previously reported differential expression...
Interactions between the intestinal microflora and host innate immune receptors play a critical role in homeostasis. Several studies have shown that TLR2 can modulate inflammatory responses gut. signals enhance tight junction formation fortify epithelial barrier, may crucial driving acute towards bacterial pathogens. In addition, agonists direct effects on both Th1 cells Treg. To define of induction regulation chronic inflammation we examined deletion several complementary models bowel...
Abstract The nonobese diabetic (NOD) mouse strain provides a good study model for Sjögren’s syndrome (SS). genetic control of SS was investigated in this using different matings, including (NOD × C57BL/6 (B6))F2 cross, NZW)F2 and ((NOD B6) NOD) backcross. Multiple loci were detected depending on parent combination sex. Despite significant complexity, two main features prominent. First, the middle region chromosome 1 (chr.1) all crosses. Its effect most visible B6)F2 cross dominated over...
The major histocompatibility complex (MHC) has long been associated with predisposition to several autoimmune diseases, including type 1 diabetes and thyroiditis. In diabetes, a primary role assigned class II genes, both in humans the nonobese diabetic (NOD) mouse model. However, an involvement of other tightly linked genes is strongly suspected. Here, through two independent sets experiments, we provide solid evidence for existence at least one such gene. First, using new recombinant...
Major histocompatibility complex (MHC) plays a largely predominant role in the genetic predisposition to type 1 diabetes, both humans and rodents. While class II loci have long been recognized as essential, they do not fully explain MHC-linked component of diabetes. In present study, using new NOD congenic strains harboring defined chromosomal segments from C57BL/6 mice, we circumscribed three distinct influencing murine diabetes tightly linked but separated region. Our findings might guide...
Objective Loss of the Crohn’s disease predisposing NOD2 gene results in an intestinal microenvironment conducive for colonisation by attaching-and-effacing enteropathogens. However, it remains elusive whether relies on intracellular recruitment serine-threonine kinase RIPK2 NOD2, a step that is required its activation transcription factor NF-κB. Design Colonisation resistance was evaluated wild type and mutant mice, as well ex-germ-free (ex-GF) mice which were colonised either with faeces...
ABSTRACT The rapid renewal of the epithelial gut lining is fuelled by stem cells that reside at base intestinal crypts. signal transduction pathways and morphogens regulate cell self-renewal differentiation have been extensively characterised. In contrast, although extracellular matrix (ECM) components form an integral part niche, their direct influence on cellular composition less well understood. We set out to systematically compare effect two ECM classes, interstitial basement membrane,...
It is believed that intestinal recruitment of monocytes from Crohn's Disease (CD) patients who carry NOD2 risk alleles may repeatedly give rise to pathogenic macrophages. We investigated an alternative possibility rather inhibit their differentiation intravasating monocytes.
Crohn's disease (CD) is a complex and poorly understood myeloid-mediated disorder. Genetic variants with loss of function in the NOD2 gene confer an increased susceptibility to ileal CD. While Nod2 myeloid cells may protection against T-cell mediated ileopathy, it remains unclear whether promote resolution inflamed colon. In this study, we evaluated model acute colitis colitis-associated colon cancer (CAC).To ablate specifically within compartment, generated LysMCre/+;Nod2fl/fl mice. The...
Over 90% of epidemic non-bacterial gastroenteritis are caused by human noroviruses (NoVs), which persist in a substantial subset people allowing their spread worldwide. This has led to significant number endemic cases and up 70,000 children deaths developing countries. NoVs primarily transmitted through the fecal-oral route. To date, studies have focused on influence gut microbiota enteric viral clearance mucosal immunity. In this study, use mouse norovirus S99 (MNoV_S99) CR6 (MNoV_CR6), two...
NOD2 has emerged as a critical player in the induction of both Th1 and Th2 responses for potentiation polarisation antigen-dependent immunity. Loss-of-function mutations NOD2-encoding gene deregulation its downstream signalling pathway have been linked to Crohn's disease. Although it is well documented that capable sensing bacterial muramyl dipeptide, remains counter-intuitive link development overt intestinal inflammation loss bacterial-induced inflammatory response. We hypothesised T...
ABSTRACT Intratumoral bacteria locally contribute to cellular and molecular tumor heterogeneity that support cancer stemness through poorly understood mechanisms. This study aims explore how Colibactin-producing Escherichia coli (CoPEC) flexibly alters the microenvironment in right-sided colorectal (CRC). Metabolomic transcriptomic spatial profiling uncovered CoPEC colonization establishes a high-glycerophospholipid within is conducive exhaustion of infiltrated CD8 + T cell has lowered...